FastQCFastQC Report
Fri 17 Jun 2016
SRR1527035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48052
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3920.8157829018563224No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2760.5743777574294514No Hit
CAACAAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCAT2570.5348372596353951No Hit
CAACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2310.4807292100224757No Hit
CAACAAGGGCAGTCACTCTCCTGGTCACAAGAGGAAGGAGACACCTAGTCCCCGTT2160.4495130275534837No Hit
CAACAAGGGGGGCTGGAGAGATGGCTCCGTGGTTAAGAGCACTGACTGCTCTTCCG1930.4016482144343627No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1910.39748605677183046No Hit
CAACAAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC1790.37251311079663696No Hit
CAACAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1650.3433780071589112No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1620.3371347706651128No Hit
CAACAAGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC1540.32048614001498377No Hit
CAACAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1520.3163239823524515No Hit
CAACAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTCC1520.3163239823524515No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1440.2996753517023225No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.2996753517023225No Hit
CAACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1400.29135103637725795No Hit
CAACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1350.2809456422209273No Hit
CAACAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1340.2788645633896612No Hit
CAACAAGGGAAGAAACACTGCTATCAAGATTTTTAAAGCTCAAACCAAGTGACACT1330.2767834845583951No Hit
CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.2705402480645967No Hit
CAACAAGGGGATCATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATG1290.2684591692333306No Hit
CAACAAGGGCAGTGCTCAACAAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGA1250.260134853908266No Hit
CAACAAGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCTTCCA1160.2414051444268709No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1060.2205943561142096No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1040.21643219845167735No Hit
CAACAAGGGCGATGTAGGACCGTATAGGTAGATCCAGACCGTGAGATGTTTTAGGG910.18937817364521767No Hit
CAACAAGGGGGGAGTTTCAGGACAGTGGGACAGTGTCCTCCTGTCTGGGGTGATGT890.18521601598268542No Hit
CAACAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC860.17897277948888704No Hit
CAACAAGGGGGACAAATAAGATTCCTGAGGAATGACTCTCTTAATTGCGCCGCGGT830.17272954299508866No Hit
CAACAAGGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTC810.1685673853325564No Hit
CAACAAGGGATGTCATTCTGTGCATCTGGGGTTATCTGGGTCTTCAGTACTCTGGG800.1664863065012903No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA780.162324148838758No Hit
CAACAAGGGACCTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC770.16024307000749188No Hit
CAACAAGGGGATTTAGCACCCGTCTAAGTTAATATAAGTAGATAAAGACTGGGACA750.15608091234495963No Hit
CAACAAGGGGCCAAAGTGCATTGCACAGAGGCCACATCTCGTGTACCTGTGGGTCG740.1539998335136935No Hit
CAACAAGGGCAAGCTAGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTG720.14983767585116126No Hit
CAACAAGGGGAAGCAGAGGCATGGGCGGAAGTGAGGGTGAAGCTGCCTCCTTGTAC710.1477565970198951No Hit
CAACAAGGGATGGCCTCTCTGGTTTCTCCTTAAGGACAAATATTTGGGATGGAGAA690.14359443935736285No Hit
CAACAAGGGCAAAATGGCTAACATAGATTTTAGAATCAAACAGTATGTGTCTTTGT660.13735120286356448No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC660.13735120286356448No Hit
CAACAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG650.13527012403229835No Hit
CAACAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.13527012403229835No Hit
CAACAAGGGCGAGCTCATGGGAAGGCTGTGCATAGGGTACTCTGAGCGCTGCCTTT630.13110796636976607No Hit
CAACAAGGGATTTCTGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCT620.12902688753849995No Hit
CAACAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.12902688753849995No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC600.1248647298759677No Hit
CAACAAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC600.1248647298759677No Hit
CAACAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG590.12278365104470157No Hit
CAACAAGGGAGGGGTTCAGCCTAGGACTCTGATGGGCTCCACTGGGGTATGGGAAA580.12070257221343544No Hit
CAACAAGGGGCAGTGCTCAACAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCT580.12070257221343544No Hit
CAACAAGGGGGGCTTTGGCTCCCCTCTTTCTCCAGGGACACGGATTGGACCACGGA570.11862149338216932No Hit
CAACAAGGGAACTTGTGTCAGTTCTCTCCTAAGGTAGCTGTCAGAGTTGTTAGTAA560.11654041455090318No Hit
CAACAAGGGCACTGAGGAAGGTTTATTTCTTGGTTGGGGTATCCTGAATGGGGCTT560.11654041455090318No Hit
CAACAAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATTCTC550.11445933571963705No Hit
CAACAAGGGGGACGCCCAGGCCGGTGTGTGTCGGTCTGTATGTGTGTGTGTGTGTG540.11237825688837093No Hit
CAACAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.11237825688837093No Hit
CAACAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.1102971780571048No Hit
CAACAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGC530.1102971780571048No Hit
CAACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTCTT510.10613502039457255No Hit
CAACAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGAAAAC510.10613502039457255No Hit
CAACAAGGGGCACTGAGGAAGGTTTATTTCTTGGTTGGGGTATCCTGAATGGGGCT500.10405394156330643No Hit
CAACAAGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGGCCCA500.10405394156330643No Hit
CAACAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA500.10405394156330643No Hit
CAACAAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGC500.10405394156330643No Hit
CAACAAGGGGGAATTTATCCCAAAGTCCCATGTTTCAACTGACCATTCCTTGACTG490.1019728627320403No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTCTTC490.1019728627320403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAC150.00800881450.5786749
ACGCACC150.00800881450.5786750
GTCCCCG309.4822644E-750.5786748
GATCTTC150.00800881450.5786749
AGGCTTC150.00800881450.5786749
CGAGCGA251.9216533E-550.5786749
CCGAGCG251.9216533E-550.5786748
TCCCCGT309.4822644E-750.5786749
TAAACGC150.00828001650.15497247
ACCATTC150.00828001650.15497242
TGACCAT150.00828001650.15497240
CCGCTTG150.00828001650.15497240
AAATATC204.0806344E-450.15497242
GTATCCT301.005048E-650.15497239
CTAAGGC355.023685E-850.15497242
GAATGGG301.005048E-650.15497246
TAGTCCC301.005048E-650.15497246
GACACCT301.005048E-650.15497240
CTGAATG301.005048E-650.15497244
TCAGTAC252.0199608E-550.15497244
Produced by FastQC (version 0.11.5)