##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527035_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48052 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.60705069508033 39.0 38.0 40.0 36.0 40.0 2 38.551652376592024 39.0 38.0 40.0 36.0 40.0 3 38.649483892449844 40.0 39.0 40.0 36.0 40.0 4 38.386060933988176 39.0 38.0 40.0 35.0 40.0 5 38.285940231415964 39.0 38.0 40.0 35.0 40.0 6 38.51843835844502 39.0 38.0 40.0 36.0 40.0 7 38.74336135852826 39.0 39.0 40.0 37.0 40.0 8 38.77878132023641 39.0 39.0 40.0 38.0 40.0 9 38.76866727711646 39.0 39.0 40.0 37.0 40.0 10 37.91180387913094 39.0 38.0 40.0 35.0 40.0 11 38.00418296845084 39.0 38.0 40.0 35.0 40.0 12 37.9635186880879 39.0 38.0 40.0 35.0 40.0 13 37.74107217181387 39.0 38.0 40.0 34.0 40.0 14 37.96867976358944 39.0 38.0 40.0 34.0 40.0 15 37.979896778489966 39.0 38.0 40.0 35.0 40.0 16 37.8274161325231 39.0 38.0 40.0 34.0 40.0 17 37.699679513859984 39.0 38.0 40.0 33.0 40.0 18 37.581078831266126 39.0 37.0 40.0 33.0 40.0 19 37.46191625738783 39.0 37.0 40.0 33.0 40.0 20 37.44004411887122 39.0 37.0 40.0 33.0 40.0 21 37.326521268625655 39.0 37.0 40.0 33.0 40.0 22 37.2067759926746 39.0 36.0 40.0 33.0 40.0 23 37.1015774577541 39.0 36.0 40.0 33.0 40.0 24 37.03949887621743 39.0 36.0 40.0 33.0 40.0 25 36.830059102638806 39.0 36.0 40.0 32.0 40.0 26 36.442811953716806 39.0 36.0 40.0 31.0 40.0 27 36.21418463331391 38.0 36.0 40.0 30.0 40.0 28 36.09810205610589 38.0 35.0 40.0 30.0 40.0 29 35.8999001082161 38.0 35.0 40.0 30.0 40.0 30 35.778094564222094 38.0 35.0 40.0 30.0 40.0 31 35.13918255223508 38.0 35.0 40.0 28.0 40.0 32 34.95398734704071 38.0 34.0 40.0 27.0 40.0 33 34.85657204694914 38.0 34.0 39.0 27.0 40.0 34 34.85166070090735 38.0 34.0 40.0 27.0 40.0 35 34.53541996170815 38.0 33.0 40.0 26.0 40.0 36 34.418026304836424 38.0 34.0 40.0 26.0 40.0 37 34.125280945642224 38.0 33.0 40.0 24.0 40.0 38 33.919108465828685 38.0 33.0 39.0 23.0 40.0 39 33.75360026637809 38.0 33.0 39.0 23.0 40.0 40 33.649962540581036 38.0 33.0 39.0 22.0 40.0 41 33.15591442603846 37.0 33.0 39.0 16.0 40.0 42 32.972883542828605 37.0 33.0 39.0 14.0 40.0 43 32.8131191209523 37.0 32.0 39.0 12.0 40.0 44 32.68767168900358 37.0 32.0 39.0 9.0 40.0 45 32.146612003662696 36.0 32.0 39.0 4.0 40.0 46 31.62669607924748 36.0 31.0 39.0 4.0 40.0 47 31.41767252143511 36.0 31.0 39.0 4.0 40.0 48 31.26494214600849 36.0 31.0 39.0 4.0 40.0 49 30.834346125031217 36.0 30.0 39.0 4.0 40.0 50 30.466557063181554 35.0 30.0 39.0 4.0 40.0 51 29.67836926662782 35.0 29.0 39.0 4.0 40.0 52 29.446495463248148 35.0 29.0 39.0 4.0 40.0 53 29.08413801714809 35.0 28.0 39.0 4.0 40.0 54 28.6239282444019 35.0 26.0 39.0 4.0 40.0 55 28.193477898942813 35.0 24.0 38.0 4.0 40.0 56 16.40780820777491 20.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.7632309602966387 1 2 -1.3499164538741297 1 3 -1.277024227384615 1 4 -1.3149109369867276 1 5 -1.4997526053879895 1 6 -0.8608586528179174 1 7 -0.9332646969447254 1 8 -1.0730642885887747 1 9 -0.9921466590429944 1 10 -1.0929531669743469 1 11 -0.8437598237216903 1 12 -0.9596870011833687 1 13 -0.9798805713069783 1 14 -1.153544412221784 1 15 -1.0012902580817382 1 16 -1.2797105902921402 1 17 -0.8673385447908402 1 18 -1.0140818366042623 1 19 -0.7785493687821372 1 20 -0.7624882686832564 1 21 -0.3077646393497844 1 22 -1.2137051515795676 1 23 -0.7182883853976776 1 24 -0.4745951832762998 1 25 -2.4971914456243383 1 26 -0.30738416228887644 1 27 -2.5719573803513356 1 28 -0.26334839364349705 1 29 0.21744870524091908 1 30 0.09594375571195002 1 31 0.2462350272112488 1 32 -0.40146788664151245 1 33 0.05140016732062236 1 34 -1.74628762708371 1 35 -0.3655973161971957 1 36 0.5042179168071783 1 37 0.8747822751694017 1 38 0.9596017264609813 1 39 0.37256664516378635 1 40 1.2943897263287667 1 41 1.5868339476195956 1 42 1.8577401540830962 1 43 0.8530867773501924 1 44 2.2870003845977678 1 45 1.2758340032099937 1 46 0.5116858881410415 1 47 1.2796451007349887 1 48 1.6170107574862378 1 49 2.030236776090902 1 50 2.3579895156855137 1 51 1.4923587632499569 1 52 2.2505743012978137 1 53 2.5546434004912406 1 54 2.4516232148940986 1 55 0.7966903130366134 1 56 2.2788736926092454 2 1 -0.44378651585218876 2 2 -0.42769423165191256 2 3 -0.40619089405127795 2 4 -0.9024109369867332 2 5 -1.4247526053879866 2 6 -0.7164142083734717 2 7 -0.3374313636113868 2 8 -0.32445317747766467 2 9 -0.33242443682077294 2 10 -1.1012865003076797 2 11 -0.23125982372169318 2 12 -0.563853667850033 2 13 -0.2562694601958668 2 14 -0.334099967777334 2 15 -0.4360124803039582 2 16 -0.4227661458476959 2 17 -0.9006718781241716 2 18 -0.593248503270928 2 19 -0.5535493687821358 2 20 -1.1791549353499207 2 21 -0.8702646393497844 2 22 -0.6512051515795676 2 23 -1.2016217187310119 2 24 -0.8884840721651841 2 25 -0.888858112291004 2 26 -0.7296063845111007 2 27 -0.421957380351337 2 28 -0.9189039491990556 2 29 -1.2686624058701952 2 30 -0.2735006887324971 2 31 -1.1787649727887555 2 32 -0.9361901088637339 2 33 -1.0708220549015977 2 34 -1.2393431826392671 2 35 -0.37531953841941856 2 36 -0.4291154165261517 2 37 -0.06688439149726833 2 38 0.3762683931276456 2 39 0.6322888673860092 2 40 -0.4944991625601247 2 41 0.28683394761959846 2 42 0.1591290429719905 2 43 -0.06219100042758896 2 44 -0.5102218376244565 2 45 -0.08388821901223054 2 46 0.3936303325854844 2 47 0.5657562118461001 2 48 1.1447885352640164 2 49 0.2955145538686814 2 50 0.14271173790773517 2 51 -0.077085681194486 2 52 0.5811298568533694 2 53 -0.043967710619870815 2 54 0.9071787704496543 2 55 0.2647458685921684 2 56 0.9260959148314676 3 1 0.17396710733621745 3 2 -0.3117522026664048 3 3 0.12732359870233978 3 4 0.009726744172688484 3 5 -0.1693178227792913 3 6 -0.22366058518506549 3 7 -0.14630817520558992 3 8 -0.10977926443418795 3 9 -0.11775052377729622 3 10 -0.17556186262651607 3 11 -0.30462938893908387 3 12 0.06205212925141268 3 13 0.003694307920078188 3 14 0.04452322062846292 3 15 -0.17423711798512187 3 16 0.4241541440073817 3 17 0.09208174506423461 3 18 0.14678772861312694 3 19 0.37942164571061454 3 20 -0.01611145708905326 3 21 0.4531049258676063 3 22 0.6123818049421743 3 23 0.1643203102544959 3 24 0.09883476841452676 3 25 0.9317940616220426 3 26 0.5385095575178838 3 27 0.0671730544312723 3 28 0.37095112326471735 3 29 1.0411202028254536 3 30 0.40222394894866653 3 31 0.25782923010979886 3 32 0.43428090562901644 3 33 0.33497504654767596 3 34 0.444533628954936 3 35 0.3484123456385504 3 36 1.0708845834738483 3 37 1.2428982171983805 3 38 0.8509060742870673 3 39 1.5208758239077511 3 40 0.38231243164277373 3 41 1.5042252519674264 3 42 1.7741652748560455 3 43 1.0129901589927002 3 44 1.241589756578442 3 45 1.5275248244660276 3 46 1.506854970266648 3 47 1.9371330234403032 3 48 2.619426216423438 3 49 3.0790290466223027 3 50 3.2541247813859933 3 51 4.1203780869214555 3 52 4.278593624969311 3 53 4.085561274887375 3 54 4.994135292188787 3 55 4.691376303374778 3 56 3.2431249003387137 4 1 -0.6521198491855245 4 2 -1.1813174200577095 4 3 -1.0683285752106997 4 4 -1.403316734088179 4 5 -1.3649699966923379 4 6 -1.5062692808372447 4 7 -1.14630817520559 4 8 -0.8271705687820159 4 9 -0.8786200889946869 4 10 -1.2407792539308673 4 11 -1.8481076498086466 4 12 -1.3292522185746733 4 13 -1.3876100399060078 4 14 -1.6293898228498023 4 15 -0.7829327701590358 4 16 -0.7280197690361021 4 17 -1.103570428848812 4 18 -0.4836470539955684 4 19 -0.7727522673328622 4 20 -1.4291549353499207 4 21 -2.4816776828280425 4 22 -1.8224008037534816 4 23 -1.3791579506150669 4 24 -1.6402956663680825 4 25 -1.981249416638832 4 26 -0.896273051177765 4 27 -0.9328269455687277 4 28 -1.2377445289091966 4 29 -1.5893145797832418 4 30 -0.29342822496438004 4 31 -2.176953378585857 4 32 -1.1091973552405463 4 33 -2.2302423447566753 4 34 -1.6206837623494081 4 35 -1.455935480448403 4 36 -2.385637155656589 4 37 -1.844058304540745 4 38 -1.4751808822346675 4 39 -1.1312980891357327 4 40 -1.3133397422702657 4 41 -2.69142692194562 4 42 -2.3780086381874312 4 43 -2.3348359279638196 4 44 -2.54101893907373 4 45 -2.9507360450991875 4 46 -1.101840681907266 4 47 -1.7367800200379548 4 48 -1.271878131402648 4 49 -0.3992318229429124 4 50 -0.5719621751357415 4 51 -0.07527408699158755 4 52 0.4525066684475725 4 53 -0.153569159895234 4 54 -0.7449951425938224 4 55 -1.569493261842613 4 56 -0.4960055344438956 5 1 -0.15211984918552446 5 2 -0.3769695939707489 5 3 -0.2857198795585276 5 4 0.09668326591182108 5 5 -0.10410043147494008 5 6 -0.6149649330111586 5 7 0.0928222595770194 5 8 0.020655518174507392 5 9 -0.3134026976903357 5 10 0.21574248519956996 5 11 0.10841408932178354 5 12 -0.3509913490094547 5 13 0.28630300357225025 5 14 -0.17286808371936502 5 15 -3.2407450685667527E-4 5 16 -0.4888893342534928 5 17 0.3312121798468439 5 18 -0.4836470539955684 5 19 -0.2510131368980808 5 20 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0.5571979065506838 6 5 0.1900433129793626 6 6 0.0012728664564605197 6 7 0.22591897652466741 6 8 0.36049580211417265 6 9 0.3525245427710644 6 10 0.06623050649504592 6 11 0.22154629872728293 6 12 -0.44948291955071795 6 13 0.7339516282395095 6 14 0.3045054743995337 6 15 -0.018070303433212587 6 16 0.3837814731999245 6 17 0.32721927833841136 6 18 0.4675508164569635 6 19 0.24321933870085388 6 20 -0.1026243231050259 6 21 1.1003986259563376 6 22 0.9495601545428798 6 23 1.0449769207247712 6 24 1.046039737358626 6 25 0.5626724999538908 6 26 1.5425024590263163 6 27 1.5219201706690697 6 28 0.9960620371955073 6 29 1.2083784104563335 6 30 1.4909550935804319 6 31 1.3509969319731496 6 32 0.8184867618845644 6 33 1.1570691015609853 6 34 1.6233268853879466 6 35 1.601296107839083 6 36 1.7341498895962957 6 37 2.2570952003394638 6 38 1.612662950950778 6 39 1.6437684592227413 6 40 0.9102627422017804 6 41 2.0521400700685746 6 42 1.7479895871896787 6 43 1.9025198839261535 6 44 1.3205604753007165 6 45 1.9148362707836881 6 46 1.2894636659188201 6 47 0.4628650553835172 6 48 1.095468807372864 6 49 1.4787628531884138 6 50 2.314905615458759 6 51 2.279206835812314 6 52 3.110891761615271 6 53 2.629926847203258 6 54 2.5704440765721017 6 55 2.646123419612575 6 56 1.1038170032668404 7 1 -0.09892835982382309 7 2 0.1715965484899371 7 3 0.19531619814730306 7 4 -0.08370526322786276 7 5 -0.38485898836670884 7 6 0.3225928838251093 7 7 0.3943948775233608 7 8 0.3657063969904186 7 9 0.44284152062603255 7 10 0.4687489606852324 7 11 -0.19269599393445702 7 12 -0.5341550862897506 7 13 0.09435110718002448 7 14 -0.10626308834471132 7 15 0.021877590618025522 7 16 -0.029592386982443486 7 17 0.031952235351006664 7 18 0.13059901445956257 7 19 0.6157768723526118 7 20 -0.19511238215843463 7 21 1.0326608925651115 7 22 0.42459272076085597 7 23 -0.03318200241895397 7 24 0.45637408386318157 7 25 0.6616738026026141 7 26 0.029258863715853067 7 27 0.25357453454228107 7 28 0.5101740649853426 7 29 0.8722950409383188 7 30 1.8018539211965816 7 31 1.8221215520339413 7 32 1.6599978343986734 7 33 0.7766956755948513 7 34 1.4570220655876867 7 35 1.7341662771834194 7 36 0.8687569238993831 7 37 1.552796459566558 7 38 1.5766230030567243 7 39 1.5777675907902662 7 40 0.40266395800725263 7 41 1.5102382029387442 7 42 1.5877637947450367 7 43 1.0809827584376634 7 44 0.9640689425173932 7 45 0.7685053980090473 7 46 0.6724423893230771 7 47 0.4602597579453942 7 48 -0.03783557821115835 7 49 1.2746812205353493 7 50 1.0908500357800754 7 51 1.4238008436282072 7 52 0.4118036157186147 7 53 1.2321847716496315 7 54 1.9816468555560363 7 55 0.7182033154006788 7 56 1.5493228651860775 8 1 -0.6937865158521888 8 2 -1.0318608983185769 8 3 -0.6145242273846137 8 4 -0.5065776036533975 8 5 -0.028919272054650946 8 6 0.1169191249598569 8 7 -0.14993136361138681 8 8 -0.09528651081100037 8 9 -0.24909110348743724 8 10 -0.16378650030767972 8 11 0.12290684294497112 8 12 -0.3555203345167044 8 13 0.13956387313746887 8 14 0.14506669888933033 8 15 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498.0 25 748.0 26 637.0 27 671.0 28 787.0 29 877.0 30 1065.0 31 1307.0 32 1470.0 33 1946.0 34 2614.0 35 3515.0 36 4985.0 37 7604.0 38 9284.0 39 7057.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.76275701323566 0.1748106218263548 0.016648630650129028 0.04578373428785482 8 99.7314402298372 0.20194029229295918 0.01457301078402798 0.052046467085814216 9 99.26121701490052 0.35378340131524183 0.21851327728294348 0.1664863065012903 10 54.38483309747774 26.710646799300758 6.384749854324482 12.519770248897027 11 31.52001997835678 25.02705402480646 17.709980854074754 25.742945142762007 12 36.31274452676267 16.41971197868975 27.9801048863731 19.28743860817448 13 24.254973778406725 14.802713726795972 31.39723632731208 29.545076167485224 14 21.22076084242071 19.605843669358194 36.25863647714975 22.91475901107134 15 24.444351952051942 20.06576209106801 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22.025247896008665 28.516373635530375 26.291559036746936 29 25.791525105779854 22.562894720386854 28.828396940200513 22.817183233632782 30 24.62979184916364 20.94648145830726 31.708588829099405 22.71513786342969 31 25.59469159502546 18.683749269676987 33.256823303563976 22.464735831733577 32 26.47163157565261 20.038394925192495 34.13809652984997 19.351876969304925 33 27.445578444263564 18.068165216120885 36.20729238411287 18.278963955502682 34 24.40913646233506 19.886838149323534 39.36028060798397 16.34374478035744 35 21.23250470022979 24.357635262168372 39.598913724670986 14.810946312930854 36 23.99657341050124 26.836046050019853 33.68922504753348 15.478155491945426 37 25.416396731520763 23.759169087375394 34.43188230131032 16.392551879793526 38 24.486766734958398 24.70209474432412 34.682443450265495 16.12869507045198 39 25.066398979442457 22.730409686931427 34.10502540937323 18.098165924252882 40 23.370725377757314 22.92704365660709 33.399606546398225 20.302624419237368 41 20.941575432793954 23.64405183060853 35.169872935462934 20.24449980113458 42 19.351799510081026 22.345748801373446 36.83604463706216 21.466407051483365 43 19.80521520578071 20.276468740182217 38.596711697559954 21.321604356477117 44 24.40352751419175 17.93711640377888 36.5466390163179 21.11271706571147 45 21.090345289976533 21.496815286624205 35.54307743881998 21.869761984579284 46 22.72641529207101 21.927857307539142 34.792815074091926 20.552912326297918 47 21.622358269037235 19.762663535726265 37.216957396846695 21.3980207983898 48 23.772073319072188 20.06627238790319 34.92932343441969 21.232330858604925 49 22.192165744002683 21.01157523905385 36.50603925515853 20.290219761784936 50 18.70109710306056 20.557571689286988 38.72165467474985 22.019676532902604 51 19.12751677852349 20.39219798657718 38.53397651006711 21.946308724832214 52 19.941262848751837 21.722257184812253 37.39039228026012 20.94608768617579 53 19.70838141193748 20.91261932235393 38.31112975978181 21.06786950592678 54 20.696144980862357 21.596988728879865 36.17331726194252 21.533549028315253 55 19.549156242129122 21.956594744353957 35.074720846276556 23.419528167240365 56 18.402938090241346 22.782791185729277 34.33158447009444 24.482686253934943 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 2.0 4 2.0 5 0.0 6 0.5 7 1.0 8 10.5 9 20.0 10 44.0 11 68.0 12 79.5 13 116.0 14 141.0 15 232.5 16 324.0 17 393.5 18 463.0 19 497.0 20 531.0 21 608.5 22 792.5 23 899.0 24 1118.0 25 1337.0 26 1479.0 27 1621.0 28 1689.5 29 1565.0 30 1372.0 31 1239.0 32 1106.0 33 1188.5 34 1271.0 35 1087.0 36 903.0 37 937.5 38 944.0 39 916.0 40 864.0 41 812.0 42 1006.5 43 1201.0 44 1347.5 45 1494.0 46 1521.0 47 1589.5 48 1631.0 49 1726.0 50 1821.0 51 1893.5 52 1966.0 53 1988.0 54 2269.5 55 2529.0 56 3272.0 57 4015.0 58 3974.5 59 3934.0 60 3625.0 61 3316.0 62 2921.5 63 1947.5 64 1368.0 65 1316.5 66 1265.0 67 949.0 68 633.0 69 539.5 70 446.0 71 457.5 72 349.0 73 229.0 74 168.0 75 107.0 76 104.5 77 102.0 78 74.5 79 46.0 80 45.0 81 41.0 82 37.0 83 23.5 84 10.0 85 8.0 86 6.0 87 3.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.037459418962790314 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0020810788312661285 21 0.0 22 0.0 23 0.0 24 0.004162157662532257 25 0.029135103637725795 26 0.07491883792558063 27 0.06867560143178224 28 0.09989178390077415 29 0.15608091234495963 30 0.2205943561142096 31 0.26637809040206445 32 0.2684591692333306 33 0.28926995754599183 34 0.324648297677516 35 0.37875634729043534 36 0.39748605677183046 37 0.41829684508449183 38 0.45367518521601596 39 0.4890535253475402 40 0.5618912844418547 41 0.5847831515857821 42 0.6014317822359111 43 0.6388912011987014 44 0.6513776741862981 45 0.6742695413302257 46 0.7096478814617497 47 0.7408640639307417 48 0.7699991675684674 49 0.7575126945808707 50 0.7928910347123949 51 0.7741613252309997 52 0.7949721135436609 53 0.8053775076999916 54 1.587863148256056 55 0.8490801631565804 56 0.8365936901689837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 48052.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.35220178140348 #Duplication Level Percentage of deduplicated Percentage of total 1 66.7100765098486 25.584783151585782 2 13.131477562537306 10.072421543328062 3 6.4300830213250855 7.398235245151086 4 3.6789842096695424 5.64388579039374 5 2.349557762222584 4.5055356696911675 6 1.51391828097021 3.483725963539499 7 1.166639535514678 3.132023641055523 8 0.8139345596614032 2.497294597519354 9 0.6565738781268653 2.2662948472488136 >10 3.2231808562591566 21.74311162906851 >50 0.18991806392099408 4.807292100224757 >100 0.1356557599435672 8.865395821193706 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 392 0.8157829018563224 No Hit CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 276 0.5743777574294514 No Hit CAACAAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCAT 257 0.5348372596353951 No Hit CAACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 231 0.4807292100224757 No Hit CAACAAGGGCAGTCACTCTCCTGGTCACAAGAGGAAGGAGACACCTAGTCCCCGTT 216 0.4495130275534837 No Hit CAACAAGGGGGGCTGGAGAGATGGCTCCGTGGTTAAGAGCACTGACTGCTCTTCCG 193 0.4016482144343627 No Hit CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 191 0.39748605677183046 No Hit CAACAAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC 179 0.37251311079663696 No Hit CAACAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 165 0.3433780071589112 No Hit CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 162 0.3371347706651128 No Hit CAACAAGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC 154 0.32048614001498377 No Hit CAACAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 152 0.3163239823524515 No Hit CAACAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTCC 152 0.3163239823524515 No Hit CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 144 0.2996753517023225 No Hit CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 144 0.2996753517023225 No Hit CAACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 140 0.29135103637725795 No Hit CAACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 135 0.2809456422209273 No Hit CAACAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 134 0.2788645633896612 No Hit CAACAAGGGAAGAAACACTGCTATCAAGATTTTTAAAGCTCAAACCAAGTGACACT 133 0.2767834845583951 No Hit CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.2705402480645967 No Hit CAACAAGGGGATCATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATG 129 0.2684591692333306 No Hit CAACAAGGGCAGTGCTCAACAAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGA 125 0.260134853908266 No Hit CAACAAGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCTTCCA 116 0.2414051444268709 No Hit CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 106 0.2205943561142096 No Hit CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 104 0.21643219845167735 No Hit CAACAAGGGCGATGTAGGACCGTATAGGTAGATCCAGACCGTGAGATGTTTTAGGG 91 0.18937817364521767 No Hit CAACAAGGGGGGAGTTTCAGGACAGTGGGACAGTGTCCTCCTGTCTGGGGTGATGT 89 0.18521601598268542 No Hit CAACAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC 86 0.17897277948888704 No Hit CAACAAGGGGGACAAATAAGATTCCTGAGGAATGACTCTCTTAATTGCGCCGCGGT 83 0.17272954299508866 No Hit CAACAAGGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTC 81 0.1685673853325564 No Hit CAACAAGGGATGTCATTCTGTGCATCTGGGGTTATCTGGGTCTTCAGTACTCTGGG 80 0.1664863065012903 No Hit CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 78 0.162324148838758 No Hit CAACAAGGGACCTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC 77 0.16024307000749188 No Hit CAACAAGGGGATTTAGCACCCGTCTAAGTTAATATAAGTAGATAAAGACTGGGACA 75 0.15608091234495963 No Hit CAACAAGGGGCCAAAGTGCATTGCACAGAGGCCACATCTCGTGTACCTGTGGGTCG 74 0.1539998335136935 No Hit CAACAAGGGCAAGCTAGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTG 72 0.14983767585116126 No Hit CAACAAGGGGAAGCAGAGGCATGGGCGGAAGTGAGGGTGAAGCTGCCTCCTTGTAC 71 0.1477565970198951 No Hit CAACAAGGGATGGCCTCTCTGGTTTCTCCTTAAGGACAAATATTTGGGATGGAGAA 69 0.14359443935736285 No Hit CAACAAGGGCAAAATGGCTAACATAGATTTTAGAATCAAACAGTATGTGTCTTTGT 66 0.13735120286356448 No Hit CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 66 0.13735120286356448 No Hit CAACAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 65 0.13527012403229835 No Hit CAACAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65 0.13527012403229835 No Hit CAACAAGGGCGAGCTCATGGGAAGGCTGTGCATAGGGTACTCTGAGCGCTGCCTTT 63 0.13110796636976607 No Hit CAACAAGGGATTTCTGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCT 62 0.12902688753849995 No Hit CAACAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.12902688753849995 No Hit CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 60 0.1248647298759677 No Hit CAACAAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 60 0.1248647298759677 No Hit CAACAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG 59 0.12278365104470157 No Hit CAACAAGGGAGGGGTTCAGCCTAGGACTCTGATGGGCTCCACTGGGGTATGGGAAA 58 0.12070257221343544 No Hit CAACAAGGGGCAGTGCTCAACAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCT 58 0.12070257221343544 No Hit CAACAAGGGGGGCTTTGGCTCCCCTCTTTCTCCAGGGACACGGATTGGACCACGGA 57 0.11862149338216932 No Hit CAACAAGGGAACTTGTGTCAGTTCTCTCCTAAGGTAGCTGTCAGAGTTGTTAGTAA 56 0.11654041455090318 No Hit CAACAAGGGCACTGAGGAAGGTTTATTTCTTGGTTGGGGTATCCTGAATGGGGCTT 56 0.11654041455090318 No Hit CAACAAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATTCTC 55 0.11445933571963705 No Hit CAACAAGGGGGACGCCCAGGCCGGTGTGTGTCGGTCTGTATGTGTGTGTGTGTGTG 54 0.11237825688837093 No Hit CAACAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.11237825688837093 No Hit CAACAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.1102971780571048 No Hit CAACAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGC 53 0.1102971780571048 No Hit CAACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTCTT 51 0.10613502039457255 No Hit CAACAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGAAAAC 51 0.10613502039457255 No Hit CAACAAGGGGCACTGAGGAAGGTTTATTTCTTGGTTGGGGTATCCTGAATGGGGCT 50 0.10405394156330643 No Hit CAACAAGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGGCCCA 50 0.10405394156330643 No Hit CAACAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 50 0.10405394156330643 No Hit CAACAAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGC 50 0.10405394156330643 No Hit CAACAAGGGGGAATTTATCCCAAAGTCCCATGTTTCAACTGACCATTCCTTGACTG 49 0.1019728627320403 No Hit CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTCTTC 49 0.1019728627320403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0020810788312661285 0.0 0.0 0.0 0.0 11 0.010405394156330641 0.0 0.0 0.0 0.0 12 0.010405394156330641 0.0 0.0 0.0 0.0 13 0.010405394156330641 0.0 0.0 0.0 0.0 14 0.014567551818862899 0.0 0.0 0.0 0.0 15 0.016648630650129028 0.0 0.0 0.0 0.0 16 0.018729709481395154 0.0 0.0 0.0 0.0 17 0.018729709481395154 0.0 0.0 0.0 0.0 18 0.018729709481395154 0.0 0.0 0.0 0.0 19 0.02289186714392741 0.0 0.0 0.0 0.0 20 0.02289186714392741 0.0 0.0 0.0 0.0 21 0.02289186714392741 0.0 0.0 0.0 0.0 22 0.02497294597519354 0.0 0.0 0.0 0.0 23 0.02497294597519354 0.0 0.0 0.0 0.0 24 0.02705402480645967 0.0 0.0 0.0 0.0 25 0.029135103637725798 0.0 0.0 0.0 0.0 26 0.03537834013152418 0.0 0.0 0.0 0.0 27 0.03537834013152418 0.0 0.0 0.0 0.0 28 0.03537834013152418 0.0 0.0 0.0 0.0 29 0.03745941896279031 0.0 0.0 0.0 0.0 30 0.041621576625322565 0.0 0.0 0.0 0.0 31 0.04578373428785482 0.0 0.0 0.0 0.0 32 0.047864813119120955 0.0 0.0 0.0 0.0 33 0.04994589195038708 0.0 0.0 0.0 0.0 34 0.05410804961291934 0.0 0.0 0.0 0.0 35 0.05410804961291934 0.0 0.0 0.0 0.0 36 0.06035128610671772 0.0 0.0 0.0 0.0 37 0.06243236493798385 0.0 0.0 0.0 0.0 38 0.06451344376924997 0.0 0.0 0.0 0.0 39 0.07075668026304836 0.0 0.0 0.0 0.0 40 0.07283775909431449 0.0 0.0 0.0 0.0 41 0.07908099558811288 0.0 0.0 0.0 0.0 42 0.081162074419379 0.0 0.0 0.0 0.0 43 0.081162074419379 0.0 0.0 0.0 0.0 44 0.08532423208191127 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCAC 15 0.008008814 50.57867 49 ACGCACC 15 0.008008814 50.57867 50 GTCCCCG 30 9.4822644E-7 50.57867 48 GATCTTC 15 0.008008814 50.57867 49 AGGCTTC 15 0.008008814 50.57867 49 CGAGCGA 25 1.9216533E-5 50.57867 49 CCGAGCG 25 1.9216533E-5 50.57867 48 TCCCCGT 30 9.4822644E-7 50.57867 49 TAAACGC 15 0.008280016 50.154972 47 ACCATTC 15 0.008280016 50.154972 42 TGACCAT 15 0.008280016 50.154972 40 CCGCTTG 15 0.008280016 50.154972 40 AAATATC 20 4.0806344E-4 50.154972 42 GTATCCT 30 1.005048E-6 50.154972 39 CTAAGGC 35 5.023685E-8 50.154972 42 GAATGGG 30 1.005048E-6 50.154972 46 TAGTCCC 30 1.005048E-6 50.154972 46 GACACCT 30 1.005048E-6 50.154972 40 CTGAATG 30 1.005048E-6 50.154972 44 TCAGTAC 25 2.0199608E-5 50.154972 44 >>END_MODULE