FastQCFastQC Report
Fri 17 Jun 2016
SRR1527034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37249
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1340.35974120110606994No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1020.27383285457327716No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC960.2577250395983785No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.21745550216113185No Hit
CTCGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA800.21477086633198209No Hit
CTCGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.1879245080404843No Hit
CTCGCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG670.17987060055303497No Hit
CTCGCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1664474214072861No Hit
CTCGCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG530.14228569894493812No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC510.13691642728663858No Hit
CTCGCAGGGATAGCATCATGGTTCTGGCCTTTTCCACATTCAATGGAAGCTTCATGGTTGTTCT490.13154715562833902No Hit
CTCGCAGGGGACTTAACAGAGTTTTCTGACTGCGTTCTGGGCAGTGAAAATAAAGGCTTAATTC460.12349324814088969No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT440.11812397648259014No Hit
CTCGCAGGGCAGTAAAGCCAAAACTACATGTCTGCAGATCTTAAGTAGCCTGATAGTCTGGGGC420.11275470482429058No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCAT420.11275470482429058No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC420.11275470482429058No Hit
CTCGCAGGGGACTCTTGATTCATTGGCATTAGATTCTACTTCTTTGGGATTCTGACATATCCTG400.10738543316599104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAT258.019959E-658.57687458
CGAGGTC150.00447255658.5768756
CAATAAT150.00447255658.5768756
AGGGGAA258.485029E-658.024266
GCAGGGC2750.058.024264
AGGGATT501.8189894E-1258.024266
AGGGATA258.485029E-658.024266
CAGGGCT452.910383E-1158.024265
GGGCATC150.00464393858.0242587
GGGCACA201.980907E-458.0242587
GCAAGGC150.00464393858.02425855
AGGGCTC150.00464393858.0242586
AGGGTGG600.058.0242586
CATCAAT150.00464393858.02425853
TCGTGAT201.980907E-458.02425837
CGCTACT150.00464393858.02425852
AGGGAGA550.058.0242586
TATCTCA201.980907E-458.02425848
AATGGTC150.00464393858.02425845
CGCAGGA303.6522033E-758.0242583
Produced by FastQC (version 0.11.5)