FastQCFastQC Report
Fri 17 Jun 2016
SRR1527033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49382
Sequences flagged as poor quality0
Sequence length64
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5971.20894252966668No Hit
CTCGCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4120.834312097525414No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3460.7006601595723138No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3450.6986351302093881No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1850.37463043214126607No Hit
CTCGCAGGGCAGTAAAGCCAAAACTACATGTCTGCAGATCTTAAGTAGCCTGATAGTCTGGGGC1810.36653031468956304No Hit
CTCGCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1580.3199546393422705No Hit
CTCGCAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTAGGGGTACG1560.3159045806164189No Hit
CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1440.29160422826130983No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCGAAGGTCC1410.2855291401725325No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.281479081446681No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC1340.27135393463205215No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1210.24502855291401726No Hit
CTCGCAGGGAAGTGTGGTGTTGCAGACGGAGAGGGTTTTCTGAGTATCTGGCAAGTTAACCAAA1200.2430035235510915No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1190.24097849418816572No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT1170.2369284354623142No Hit
CTCGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1100.22275322992183388No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1070.2166781418330566No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1050.21262808310720505No Hit
CTCGCAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT910.18427767202624437No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTCTTCCGAAGGTC850.1721274958486898No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC850.1721274958486898No Hit
CTCGCAGGGGACTTAACAGAGTTTTCTGACTGCGTTCTGGGCAGTGAAAATAAAGGCTTAATTC840.17010246648576405No Hit
CTCGCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.16402737839698675No Hit
CTCGCAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT780.15795229030820945No Hit
CTCGCAGGGACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTT780.15795229030820945No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCAT770.15592726094528372No Hit
CTCGCAGGGATCCCCAAGGTCTTAGCCATGTCTGGAGACCCTAACTACCTGCACCGAATGACTA730.14782714349358064No Hit
CTCGCAGGGCACCTCGCTGGGCGTTGAGCAAACTGGGAGGTATAAGCCCACCTGCTGACCTGAT720.14580211413065491No Hit
CTCGCAGGGACCTCAGGTGTCACCAGCACTTTTGACCAAGTCCTGTTACTGTGGCCCCTGTCTC720.14580211413065491No Hit
CTCGCAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC700.14175205540480337No Hit
CTCGCAGGGATCTTCGCCTGCGTGGTCACCCACCTGACCCTTCTTTCTCAAGGAAGCAACTTCC690.13972702604187762No Hit
CTCGCAGGGGGACTATGTGCCGTCAGCAGGCACCATCTCACCCACCTCTTCCCTGGAAGAAGAC690.13972702604187762No Hit
CTCGCAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGACTTGGTCC680.13770199667895183No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCAT680.13770199667895183No Hit
CTCGCAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG680.13770199667895183No Hit
CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCGAAGGTC640.1296018792272488No Hit
CTCGCAGGGGTCCCTCCTCCAGTAACTGCACTTGCCCCTCACAACCCACGGCAGCTTGGCTTCC640.1296018792272488No Hit
CTCGCAGGGGTTACCATTAATTCTTCAAACTGGCTTCCTCCTTAAAGTAAACCTGGCCCAAGTG620.12555182050139727No Hit
CTCGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.12555182050139727No Hit
CTCGCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG620.12555182050139727No Hit
CTCGCAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTCTACTG620.12555182050139727No Hit
CTCGCAGGGGGCCTCAGAATAGCATGGGCCATGGTAGTGGAGTAAAGAAAGGTCAGGCAAAAGG610.12352679113847151No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA600.12150176177554575No Hit
CTCGCAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGG590.11947673241261998No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC590.11947673241261998No Hit
CTCGCAGGGATAGCATCATGGTTCTGGCCTTTTCCACATTCAATGGAAGCTTCATGGTTGTTCT590.11947673241261998No Hit
CTCGCAGGGGATCAAGGAGAAATGGATGTCTATGTCAATAATGAATGGGCAACCAGTGTTGGGG570.11542667368676847No Hit
CTCGCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG570.11542667368676847No Hit
CTCGCAGGGGTGCTCTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC560.1134016443238427No Hit
CTCGCAGGGGCTGAAGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCGAGAGACTCAT550.11137661496091694No Hit
CTCGCAGGGGACTCTTGATTCATTGGCATTAGATTCTACTTCTTTGGGATTCTGACATATCCTG540.10935158559799117No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTTTGCTCTTCCAGAGG530.1073265562350654No Hit
CTCGCAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCAACAT530.1073265562350654No Hit
CTCGCAGGGGGCTCAGTTTGATACGGGAAGAAACCCCAAAGTATTTGGATTAGAAACAAGTCGT530.1073265562350654No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC530.1073265562350654No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA520.10530152687213964No Hit
CTCGCAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT520.10530152687213964No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTTCCGAAGGTCC510.10327649750921387No Hit
CTCGCAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCA510.10327649750921387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTC258.531748E-658.01825730
TGGTCCT150.00465491658.01825744
AAGTAGC258.531748E-658.01825743
ACAGATG150.00465491658.01825756
CCTGTTG150.00465491658.01825739
CCAAATT150.00465491658.01825730
GGGTATC150.00465491658.0182577
TAAGCAT150.00465491658.01825746
TAAGCAC150.00465491658.01825734
TAAGTAG258.531748E-658.01825742
TTCTGTC150.00465491658.01825757
AGGGTAT150.00465491658.0182576
CGTAGTG150.00465491658.01825739
ACACCAA150.00465491658.01825739
ACACTAC150.00465491658.01825744
ACCAAAT150.00465491658.01825729
ACCCGAT150.00465491658.01825742
CCCACGG150.00465491658.01825745
CTGATAG258.531748E-658.01825750
CCGCACT150.00465491658.01825751
Produced by FastQC (version 0.11.5)