FastQCFastQC Report
Fri 17 Jun 2016
SRR1527032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49554
Sequences flagged as poor quality0
Sequence length64
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC6021.214836340154175No Hit
CTCGCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4150.8374702344916657No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3770.7607862130201396No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2930.5912741655567664No Hit
CTCGCAGGGCAGTAAAGCCAAAACTACATGTCTGCAGATCTTAAGTAGCCTGATAGTCTGGGGC2030.4096541147031521No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1780.3592041005771482No Hit
CTCGCAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTAGGGGTACG1470.2966460830609033No Hit
CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1410.2845380796706623No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC1400.28252007910562216No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCGAAGGTCC1400.28252007910562216No Hit
CTCGCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1300.2623400734552206No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT1210.24417806836985914No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.23812406667473868No Hit
CTCGCAGGGAAGTGTGGTGTTGCAGACGGAGAGGGTTTTCTGAGTATCTGGCAAGTTAACCAAA1170.23610606610969853No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1140.23005206441457804No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1100.22198006215441737No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1010.20381805706905595No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT900.18162005085361424No Hit
CTCGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA880.17758404972353392No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCAT770.15538604350809218No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTCTTCCGAAGGTC740.14933204181297172No Hit
CTCGCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.1452960406828914No Hit
CTCGCAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGG710.14327804011785122No Hit
CTCGCAGGGATCTTCGCCTGCGTGGTCACCCACCTGACCCTTCTTTCTCAAGGAAGCAACTTCC700.14126003955281108No Hit
CTCGCAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT700.14126003955281108No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC700.14126003955281108No Hit
CTCGCAGGGACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTT690.13924203898777093No Hit
CTCGCAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT690.13924203898777093No Hit
CTCGCAGGGGACTTAACAGAGTTTTCTGACTGCGTTCTGGGCAGTGAAAATAAAGGCTTAATTC670.1352060378576906No Hit
CTCGCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG640.12915203616257012No Hit
CTCGCAGGGGGCTCAGTTTGATACGGGAAGAAACCCCAAAGTATTTGGATTAGAAACAAGTCGT630.12713403559752998No Hit
CTCGCAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT620.1251160350324898No Hit
CTCGCAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGACTTGGTCC610.12309803446744964No Hit
CTCGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.12309803446744964No Hit
CTCGCAGGGGGACTATGTGCCGTCAGCAGGCACCATCTCACCCACCTCTTCCCTGGAAGAAGAC600.1210800339024095No Hit
CTCGCAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC600.1210800339024095No Hit
CTCGCAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG600.1210800339024095No Hit
CTCGCAGGGGTCCCTCCTCCAGTAACTGCACTTGCCCCTCACAACCCACGGCAGCTTGGCTTCC590.11906203333736934No Hit
CTCGCAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCA590.11906203333736934No Hit
CTCGCAGGGGCTGAAGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCGAGAGACTCAT570.11502603220728902No Hit
CTCGCAGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT560.11300803164224886No Hit
CTCGCAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCAACAT560.11300803164224886No Hit
CTCGCAGGGATAGCATCATGGTTCTGGCCTTTTCCACATTCAATGGAAGCTTCATGGTTGTTCT550.11099003107720869No Hit
CTCGCAGGGACCTCAGGTGTCACCAGCACTTTTGACCAAGTCCTGTTACTGTGGCCCCTGTCTC550.11099003107720869No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCAT530.10695402994712838No Hit
CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCGAAGGTC520.10493602938208822No Hit
CTCGCAGGGGTGCTCTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC520.10493602938208822No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA510.10291802881704808No Hit
CTCGCAGGGATCCCCAAGGTCTTAGCCATGTCTGGAGACCCTAACTACCTGCACCGAATGACTA510.10291802881704808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATC150.00452385858.4377520
AAACTAC351.5061232E-858.4377521
CAGCATT201.9186949E-458.4377526
GATACGG258.174367E-658.4377520
TTGTACC150.00452385858.4377521
TACGGGA258.174367E-658.4377522
CCCACAC150.00452385858.4377520
ATACGGG258.174367E-658.4377521
AACTACA351.5061232E-858.4377522
GCAGACG201.9186949E-458.4377522
ACGGGAA258.174367E-658.4377523
AGTTTTC201.9186949E-458.4377520
AGATCCC150.00452385858.4377522
GTGTAAA201.9284122E-458.3781939
AGTATCT201.9284122E-458.3781943
TATCTGG201.9284122E-458.3781945
ACAAGCC201.9284122E-458.3781941
AAGCCCG201.9284122E-458.3781943
GTATTTG258.224057E-658.37818541
TTAAGTA351.5183105E-858.37818541
Produced by FastQC (version 0.11.5)