SRR1527031_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527031_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44581
Sequences flagged as poor quality	0
Sequence length	56
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	570	1.2785715887934321	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	490	1.0991229447522488	No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	459	1.0295865951862901	No Hit
CTCGCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	334	0.749198088871941	No Hit
CTCGCAGGGCAGTAAAGCCAAAACTACATGTCTGCAGATCTTAAGTAGCCTGATAG	183	0.4104887732442072	No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	172	0.38581458468854446	No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA	140	0.31403512707207104	No Hit
CTCGCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	137	0.30730580292052667	No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	132	0.29609026266795274	No Hit
CTCGCAGGGAAGTGTGGTGTTGCAGACGGAGAGGGTTTTCTGAGTATCTGGCAAGT	129	0.2893609385164084	No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC	129	0.2893609385164084	No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	123	0.2759022902133196	No Hit
CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	121	0.27141607411228996	No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	121	0.27141607411228996	No Hit
CTCGCAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	104	0.2332832372535385	No Hit
CTCGCAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTA	104	0.2332832372535385	No Hit
CTCGCAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	89	0.1996366164958166	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT	87	0.195150400394787	No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTG	85	0.19066418429375742	No Hit
CTCGCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	84	0.18842107624324264	No Hit
CTCGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	78	0.17496242794015388	No Hit
CTCGCAGGGGACTTAACAGAGTTTTCTGACTGCGTTCTGGGCAGTGAAAATAAAGG	78	0.17496242794015388	No Hit
CTCGCAGGGACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGC	78	0.17496242794015388	No Hit
CTCGCAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG	77	0.17271931988963907	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	71	0.15926067158655033	No Hit
CTCGCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC	70	0.15701756353603552	No Hit
CTCGCAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC	69	0.15477445548552074	No Hit
CTCGCAGGGGGCGCTCGCTGCGTCGACGTGCTGACGACAGGACGCCCAGGCCGGGG	68	0.15253134743500593	No Hit
CTCGCAGGGATCCCCAAGGTCTTAGCCATGTCTGGAGACCCTAACTACCTGCACCG	68	0.15253134743500593	No Hit
CTCGCAGGGACCTCAGGTGTCACCAGCACTTTTGACCAAGTCCTGTTACTGTGGCC	66	0.14804513133397637	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTCTTC	65	0.14580202328346156	No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	64	0.14355891523294678	No Hit
CTCGCAGGGTATGTTCATGATGTACATGGGCATGTGGATGTTTGGTTTCAAATGAA	61	0.13682959108140239	No Hit
CTCGCAGGGCACCTCGCTGGGCGTTGAGCAAACTGGGAGGTATAAGCCCACCTGCT	61	0.13682959108140239	No Hit
CTCGCAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAG	61	0.13682959108140239	No Hit
CTCGCAGGGGGCCTCAGAATAGCATGGGCCATGGTAGTGGAGTAAAGAAAGGTCAG	59	0.1323433749803728	No Hit
CTCGCAGGGGCTCCTCCTCCTCGCCCGGCCCCAGCTGCCAGCACCATGTCCGCAGG	58	0.13010026692985802	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC	57	0.12785715887934324	No Hit
CTCGCAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	57	0.12785715887934324	No Hit
CTCGCAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGA	56	0.12561405082882843	No Hit
CTCGCAGGGGAGGTCCTGGGGGTGTTGTTCAGCTGCAGTGCACTCTTAATTCAGGC	55	0.12337094277831363	No Hit
CTCGCAGGGGGACTATGTGCCGTCAGCAGGCACCATCTCACCCACCTCTTCCCTGG	55	0.12337094277831363	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTAC	52	0.11664161862676925	No Hit
CTCGCAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGT	52	0.11664161862676925	No Hit
CTCGCAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC	51	0.11439851057625444	No Hit
CTCGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.11215540252573968	No Hit
CTCGCAGGGGTCCCTCCTCCAGTAACTGCACTTGCCCCTCACAACCCACGGCAGCT	50	0.11215540252573968	No Hit
CTCGCAGGGAGTAAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.11215540252573968	No Hit
CTCGCAGGGGGCTCAGTTTGATACGGGAAGAAACCCCAAAGTATTTGGATTAGAAA	50	0.11215540252573968	No Hit
CTCGCAGGGATCTTCGCCTGCGTGGTCACCCACCTGACCCTTCTTTCTCAAGGAAG	50	0.11215540252573968	No Hit
CTCGCAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	49	0.10991229447522488	No Hit
CTCGCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	48	0.10766918642471009	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC	47	0.10542607837419529	No Hit
CTCGCAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGC	47	0.10542607837419529	No Hit
CTCGCAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	47	0.10542607837419529	No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCT	46	0.10318297032368048	No Hit
CTCGCAGGGGTGCAGTGCTCTCGCAGAAGTGAAGGCCCTCTGATGGACTTAAGTGT	46	0.10318297032368048	No Hit
CTCGCAGGGATAGCATCATGGTTCTGGCCTTTTCCACATTCAATGGAAGCTTCATG	45	0.10093986227316569	No Hit
CTCGCAGGGAGTCAAGAGTTTAGACTGCTCCAGCTGGGACATCCAGTCCTCGCTGC	45	0.10093986227316569	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTTT	15	0.008072087	50.47219	48
CCTGATA	25	1.9435221E-5	50.47219	49
CTGATAG	25	1.9435221E-5	50.47219	50
GCCTGAT	25	1.9435221E-5	50.47219	48
CAAGCGC	40	2.3756002E-9	50.47219	50
GTCGGAG	20	3.9522685E-4	50.47219	48
AAGTATT	15	0.008329015	50.074326	39
GTTGAGC	20	4.1100135E-4	50.074326	41
AAGTAGC	25	2.0369383E-5	50.074326	43
TAGTGAT	15	0.008329015	50.074326	42
GCTAGTG	15	0.008329015	50.074326	40
GAAACGG	15	0.008329015	50.074326	46
ACTGAGT	15	0.008329015	50.074326	43
TTCGTCT	15	0.008329015	50.074326	36
AGGATTT	20	4.1100135E-4	50.074326	35
TAAGTAG	25	2.0369383E-5	50.074326	42
GGATTTG	15	0.008329015	50.074326	36
TGATTAC	15	0.008329015	50.074326	45
TGCTGAA	15	0.008329015	50.074326	41
CGTAGTG	15	0.008329015	50.074326	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content