##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527031_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44581 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.77786500975752 39.0 39.0 40.0 37.0 40.0 2 38.57001861779682 39.0 38.0 40.0 36.0 40.0 3 38.58921962270922 39.0 38.0 40.0 36.0 40.0 4 38.23496556829142 39.0 38.0 40.0 35.0 40.0 5 38.29878199232857 39.0 38.0 40.0 35.0 40.0 6 37.99398847042462 39.0 38.0 40.0 35.0 40.0 7 38.78333819340077 39.0 39.0 40.0 38.0 40.0 8 38.77485924496983 39.0 39.0 40.0 38.0 40.0 9 38.760256611560976 39.0 39.0 40.0 38.0 40.0 10 38.02456203315314 39.0 38.0 40.0 35.0 40.0 11 38.00634799578296 39.0 38.0 40.0 35.0 40.0 12 37.84823130930217 39.0 38.0 40.0 35.0 40.0 13 37.62513178259797 39.0 38.0 40.0 34.0 40.0 14 37.860231937372426 39.0 38.0 40.0 34.0 40.0 15 37.90504923622171 39.0 38.0 40.0 35.0 40.0 16 37.75581525762096 39.0 38.0 40.0 34.0 40.0 17 37.525291043269554 39.0 38.0 40.0 33.0 40.0 18 37.33238375092528 39.0 37.0 40.0 33.0 40.0 19 37.28052309279738 39.0 37.0 40.0 33.0 40.0 20 37.233754289944144 39.0 37.0 40.0 33.0 40.0 21 37.08878221663937 39.0 36.0 40.0 33.0 40.0 22 36.94818420403311 39.0 36.0 40.0 33.0 40.0 23 36.778336062448126 39.0 36.0 40.0 32.0 40.0 24 36.74338843902111 39.0 36.0 40.0 32.0 40.0 25 36.613871380184385 38.0 36.0 40.0 31.0 40.0 26 36.20039927323299 38.0 36.0 40.0 31.0 40.0 27 35.97014423184765 38.0 35.0 40.0 30.0 40.0 28 35.80388506314349 38.0 35.0 40.0 30.0 40.0 29 35.60817388573608 38.0 35.0 39.0 29.0 40.0 30 35.351248289630114 38.0 35.0 39.0 29.0 40.0 31 34.54556874004621 38.0 33.0 39.0 27.0 40.0 32 34.516789663758104 38.0 33.0 39.0 27.0 40.0 33 34.38294340638389 38.0 33.0 39.0 26.0 40.0 34 34.1450842287073 37.0 33.0 39.0 25.0 40.0 35 33.910432695542944 37.0 33.0 39.0 25.0 40.0 36 33.82501514097934 37.0 33.0 39.0 23.0 40.0 37 33.68509006078823 37.0 33.0 39.0 23.0 40.0 38 33.30288688006101 37.0 33.0 39.0 22.0 40.0 39 33.20701644198201 36.0 33.0 39.0 21.0 40.0 40 32.96238307799287 36.0 32.0 39.0 19.0 40.0 41 32.56840358000045 36.0 32.0 39.0 10.0 40.0 42 32.35981696238308 36.0 32.0 39.0 9.0 40.0 43 32.12801417644288 36.0 31.0 39.0 4.0 40.0 44 32.00773872277428 36.0 31.0 39.0 4.0 40.0 45 31.572844933940466 36.0 31.0 39.0 4.0 40.0 46 30.765954106009286 35.0 30.0 39.0 4.0 40.0 47 30.510643547699694 35.0 30.0 39.0 4.0 40.0 48 30.30353738139566 35.0 29.0 38.0 4.0 40.0 49 30.033399878872164 35.0 29.0 38.0 4.0 40.0 50 29.645633790179673 35.0 29.0 38.0 4.0 40.0 51 28.588883156501648 34.0 27.0 38.0 4.0 40.0 52 28.283506426504566 34.0 25.0 38.0 4.0 39.0 53 27.990130324577734 34.0 24.0 38.0 4.0 39.0 54 27.56723716381418 33.0 22.0 38.0 4.0 39.0 55 27.165900271416074 33.0 16.0 38.0 4.0 39.0 56 16.032614791054485 20.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.1887335646091728 1 2 0.20688308237256336 1 3 0.4393635914251277 1 4 0.4340330135290742 1 5 -0.22349754408447353 1 6 -0.5709972072948872 1 7 -0.07609855838045121 1 8 -0.8023154001002055 1 9 -0.6758982925215449 1 10 -0.02319648165904198 1 11 -0.5965054656015809 1 12 -1.3937451943600578 1 13 -0.6296034519853109 1 14 -0.5971186671850148 1 15 -0.2286762148623609 1 16 0.3493379806276309 1 17 0.13239654682628554 1 18 -0.1017233268929445 1 19 0.3097660894752323 1 20 0.5098771035139862 1 21 0.7657027350458634 1 22 -0.13263330835575005 1 23 0.272308968802804 1 24 -0.17858257758221896 1 25 -0.35966879563610377 1 26 1.0101408807414742 1 27 0.4449292538442009 1 28 -0.014136565433631176 1 29 0.7627786840324475 1 30 -0.44692770036130725 1 31 0.2403395682886611 1 32 0.4093166142845135 1 33 -0.01896725470874827 1 34 -2.3983606020973838 1 35 -1.8751773907624667 1 36 -1.1618850023814886 1 37 -0.20017919234459924 1 38 -0.7654520580260211 1 39 -1.1252971021526932 1 40 -0.36755117361195033 1 41 0.003800733570656689 1 42 0.06866996688768268 1 43 -0.4345117109739789 1 44 -1.010790578457346 1 45 0.3471291548710269 1 46 1.0194930663654382 1 47 0.6435150323322603 1 48 0.8354058756416762 1 49 1.3119412571228786 1 50 1.8767775733719212 1 51 2.7133736965155997 1 52 2.8483536650056536 1 53 3.2615623734590784 1 54 4.206365037312562 1 55 2.3388438372295752 1 56 3.7572166993381657 2 1 -0.4089716598472677 2 2 -1.3050216795321958 2 3 -1.0606364085748723 2 4 -1.6088241293280703 2 5 -0.4223070678939962 2 6 -0.4769495882472654 2 7 -0.49157474885664243 2 8 -0.3808868286716347 2 9 -0.4973268639501143 2 10 -0.6755774340399938 2 11 -1.203648322744435 2 12 -0.24969757531243886 2 13 0.5430155956337401 2 14 -0.4090234290897712 2 15 -0.9965333577195068 2 16 -0.1697096384199881 2 17 0.21453940396914106 2 18 0.032800482630868544 2 19 0.49190894661808926 2 20 0.29916281779970433 2 21 -0.07120202685889865 2 22 -1.170728546450988 2 23 -0.9276910311971989 2 24 -0.1047730537726963 2 25 -0.8251449861122921 2 26 -1.356525785925193 2 27 -0.48721360329865604 2 28 -0.5748508511479145 2 29 0.0794453506991104 2 30 0.17092944249584008 2 31 -0.04775566980657686 2 32 0.5807451857130843 2 33 0.6715089357674415 2 34 0.5314013026645199 2 35 0.3450607044756282 2 36 0.7714483309518485 2 37 1.3950589028934957 2 38 1.5809765134025469 2 39 1.0735124216568295 2 40 0.981258350197578 2 41 1.8788007335706567 2 42 0.26152710974482574 2 43 0.8464406699783993 2 44 1.0570665643997934 2 45 1.0161767739186445 2 46 0.28020735207972436 2 47 0.44827693709416394 2 48 0.7830249232607223 2 49 0.29765554283716256 2 50 -0.1327462361518883 2 51 1.6693260774679786 2 52 1.9412108078627952 2 53 0.8175147544114587 2 54 0.8611269420744669 2 55 2.1507485991343387 2 56 1.3048357469572132 3 1 -0.09349546937107789 3 2 -0.24549787000838563 3 3 -0.393969741908208 3 4 -0.1850146055185462 3 5 -0.8187356393225684 3 6 -0.11861625491393113 3 7 0.09056810828621309 3 8 0.031017933233123074 3 9 0.06219694557369593 3 10 -0.02319648165904198 3 11 -0.7393626084587197 3 12 -0.46517376578862724 3 13 0.10849178610992993 3 14 -0.5971186671850148 3 15 0.1522761660900187 3 16 0.4445760758657258 3 17 -0.7247463103165757 3 18 -0.006485231654849599 3 19 2.4227995142211967E-4 3 20 0.8194009130377964 3 21 0.694274163617294 3 22 0.9149857392632939 3 23 0.058023254517088674 3 24 0.9642745652749269 3 25 0.14033120436389623 3 26 0.34347421407480994 3 27 -0.0788802699653175 3 28 0.5572920059949382 3 29 0.19135011260387103 3 30 -0.44692770036130725 3 31 0.026053854002945798 3 32 -0.9240167190488222 3 33 0.9334136976722007 3 34 0.8873536836169009 3 35 0.4819654663803874 3 36 -0.09045643095291211 3 37 1.8712493790839702 3 38 1.4488336562596942 3 39 0.9699409930854017 3 40 1.4419726359118599 3 41 1.5276102573801822 3 42 1.830574728792449 3 43 1.4702501937879262 3 44 1.2273046596378911 3 45 2.0376053453472167 3 46 1.0671121139844857 3 47 1.0958959847132128 3 48 1.2877868280226288 3 49 0.5500364952181158 3 50 0.7339204305147788 3 51 1.1895641727060742 3 52 1.0150203316723214 3 53 -0.857485245588542 3 54 0.8968412277887516 3 55 0.7912247896105278 3 56 -0.00468806256659704 4 1 -0.23635261222821669 4 2 -0.5074026319131448 4 3 -0.5130173609558213 4 4 -0.5421574626614074 4 5 -0.24730706789399903 4 6 -0.42814006443774133 4 7 -0.1713366536185461 4 8 -0.5642201620049718 4 9 -0.3663744829977347 4 10 -0.7374821959447502 4 11 0.046351677255564994 4 12 0.32054051992565746 4 13 -0.27246059484244967 4 14 -0.8352139052802485 4 15 0.2713237851376391 4 16 -0.12685249556284361 4 17 -0.03427011984038586 4 18 0.0649433397737198 4 19 -1.2854720057628626 4 20 -1.1329800393431526 4 21 -0.9485829792398448 4 22 -0.34691902264146535 4 23 -0.4895957931019552 4 24 -1.7976301966298394 4 25 -0.14538308135038847 4 26 0.29585516645575893 4 27 0.5639768728918213 4 28 -0.10937466067172608 4 29 0.3103977316514914 4 30 0.5054532520196489 4 31 0.335577663526756 4 32 0.028364233332133892 4 33 -0.18563392137541257 4 34 -0.5174082211450042 4 35 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37 1.087366595201189 120 38 0.4213611287871615 120 39 1.9497944729388834 120 40 1.5152327091719329 120 41 -0.11158388181395651 120 42 0.3525527507704709 120 43 0.6387483622860941 120 44 0.542322974656205 120 45 0.6456639534058297 120 46 1.5872586341310075 120 47 0.00981539863262526 120 48 0.7548197950555959 120 49 1.2771427223243421 120 50 2.387766584360932 120 51 2.2353517184936216 120 52 1.7860826027345915 120 53 1.7835403954371003 120 54 2.8162551472026713 120 55 -0.46335396496822767 120 56 -0.18234374022227406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 12.0 5 2.0 6 9.0 7 15.0 8 16.0 9 13.0 10 21.0 11 40.0 12 55.0 13 69.0 14 97.0 15 154.0 16 167.0 17 193.0 18 299.0 19 324.0 20 320.0 21 397.0 22 394.0 23 423.0 24 598.0 25 615.0 26 624.0 27 713.0 28 852.0 29 932.0 30 1057.0 31 1294.0 32 1586.0 33 2058.0 34 2752.0 35 3689.0 36 5284.0 37 7027.0 38 8053.0 39 4427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.62540095556403 0.24449877750611246 0.024674188555662724 0.10542607837419529 8 99.75991742954587 0.1795009872554299 0.03590019745108598 0.024681385747621614 9 99.20145353401674 0.5293734999214912 0.07402256566698819 0.195150400394787 10 65.58399318095152 21.95778470648931 4.203584486664723 8.25463762589444 11 40.956909894349614 20.53341109441242 16.006819048473563 22.502859962764408 12 42.14575716112245 12.222695767255107 25.1250532738162 20.50649379780624 13 28.99890087705525 14.232520580516365 26.251093515174627 30.51748502725376 14 23.045692110988984 16.670779031425944 35.95926515780265 24.32426369978242 15 26.25333662322514 19.48139341872098 32.69329983625312 21.571970121800767 16 34.234315066956775 18.992395863708754 25.394226239877977 21.379062829456494 17 35.77084408155941 18.586393306565576 21.033624189677216 24.609138422197795 18 32.069715798210005 20.48181960925058 24.92093044121935 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29.959723691019956 24.51454672276849 32 35.377899781742904 17.37506468960241 27.043628917939834 20.203406610714847 33 35.411134614519106 16.29911337143886 28.98870336198749 19.301048652054547 34 27.603205330212038 19.168955116373297 36.10723450231846 17.120605051096206 35 22.394613583138174 26.34435236894253 34.98468744370384 16.276346604215455 36 23.647339730594226 29.54903815830968 28.472316078749383 18.331306032346713 37 28.54568390701027 27.133267255361325 25.85375743377185 18.46729140385655 38 27.335827286248705 27.741470230315052 27.117230810835174 17.805471672601072 39 29.87171104547607 23.565485987419116 26.91587942191058 19.646923545194237 40 27.479865544701877 20.804475827373835 26.35188485573127 25.36377377219302 41 24.08059203104553 22.26885068363341 28.972068047470785 24.678489237850275 42 20.42561834266113 23.28263224408738 29.204278750677016 27.08747066257447 43 20.62588904694168 20.745557587662848 30.145182776761725 28.483370588633743 44 24.902917005328277 17.854240043348685 30.109274812607246 27.1335681387158 45 25.662797524953707 19.201933065353867 28.835644279842825 26.299625129849602 46 25.406577520780626 22.27367184676545 29.644018792916516 22.675731839537406 47 22.33020680302859 20.239575093230876 32.41270200022601 25.01751610351452 48 25.14584181250848 19.93623660290327 31.452087007642564 23.46583457694569 49 26.36873276368733 18.124689181246893 30.290700302907002 25.21587775215878 50 22.758839097880426 17.587712353247223 32.19173434071527 27.46171420815708 51 21.554570536885432 19.075941924103308 34.08114342575422 25.288344113257043 52 20.912977582735763 20.171013640373697 33.487909154658766 25.428099622231777 53 20.413886690037316 20.108560443288475 35.46307814090241 24.014474725771798 54 20.58950919825837 19.43830214056124 34.686666514692135 25.285522146488248 55 21.489510964267126 20.742718776166015 29.914684650026025 27.853085609540834 56 22.087208381417874 21.526033534722693 26.31638494784242 30.070373136017015 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 3.0 7 6.0 8 3.0 9 0.0 10 0.5 11 1.0 12 3.5 13 11.0 14 16.0 15 14.5 16 13.0 17 45.0 18 77.0 19 101.5 20 126.0 21 157.5 22 210.0 23 231.0 24 264.0 25 297.0 26 355.0 27 413.0 28 590.0 29 802.5 30 838.0 31 825.0 32 812.0 33 941.0 34 1070.0 35 897.5 36 725.0 37 737.0 38 686.5 39 624.0 40 639.5 41 655.0 42 752.5 43 850.0 44 850.0 45 850.0 46 998.5 47 1172.5 48 1198.0 49 1192.5 50 1187.0 51 1263.5 52 1340.0 53 1557.0 54 1889.5 55 2005.0 56 2045.5 57 2086.0 58 2278.5 59 2471.0 60 3040.5 61 3610.0 62 3803.0 63 4205.0 64 4414.0 65 3917.0 66 3420.0 67 2673.0 68 1926.0 69 1659.0 70 1392.0 71 1222.5 72 808.5 73 564.0 74 560.0 75 556.0 76 506.5 77 457.0 78 373.0 79 241.5 80 194.0 81 142.5 82 91.0 83 69.5 84 48.0 85 37.5 86 27.0 87 20.5 88 9.0 89 4.0 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.029160404656692313 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00672932415154438 25 0.017944864404118348 26 0.06505013346492901 27 0.05607770126286984 28 0.10318297032368048 29 0.15926067158655033 30 0.25122810165765685 31 0.3095489109710415 32 0.3095489109710415 33 0.32076445122361547 34 0.34768174782979294 35 0.38805769273905927 36 0.4194612054462664 37 0.4239474215472959 38 0.46432336645656225 39 0.5114286355173729 40 0.5697494448307575 41 0.5809649850833315 42 0.6033960655884794 43 0.6549875507503197 44 0.6482582265987752 45 0.6706893071039232 46 0.6931203876090711 47 0.7536843049729706 48 0.7963033579327516 49 0.7693860613265742 50 0.8389224108925328 51 0.81424822233687 52 0.8389224108925328 53 0.8209775464884144 54 1.6015791480675625 55 0.879298355801799 56 0.8703259235997398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 44581.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.435656445570984 #Duplication Level Percentage of deduplicated Percentage of total 1 67.44674642544499 25.923599739799467 2 12.407353370294718 9.5376954307889 3 6.034432448205427 6.958121172696889 4 3.61248905748468 5.553935533074628 5 2.5620075868106214 4.9236221708799714 6 1.7216224102713742 3.9703012494111842 7 1.2022176831047564 3.2345618088423316 8 0.8462211847096587 2.60200533859716 9 0.5777648088707324 1.9986092730086806 >10 3.3265246571345197 23.229626971131196 >50 0.16924423694193172 4.33368475359458 >100 0.08754012255617158 6.455664969381575 >500 0.005836008170411439 1.2785715887934321 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 570 1.2785715887934321 No Hit CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 490 1.0991229447522488 No Hit CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 459 1.0295865951862901 No Hit CTCGCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 334 0.749198088871941 No Hit CTCGCAGGGCAGTAAAGCCAAAACTACATGTCTGCAGATCTTAAGTAGCCTGATAG 183 0.4104887732442072 No Hit CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 172 0.38581458468854446 No Hit CTCGCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA 140 0.31403512707207104 No Hit CTCGCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 137 0.30730580292052667 No Hit CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 132 0.29609026266795274 No Hit CTCGCAGGGAAGTGTGGTGTTGCAGACGGAGAGGGTTTTCTGAGTATCTGGCAAGT 129 0.2893609385164084 No Hit CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC 129 0.2893609385164084 No Hit CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 123 0.2759022902133196 No Hit CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 121 0.27141607411228996 No Hit CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 121 0.27141607411228996 No Hit CTCGCAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 104 0.2332832372535385 No Hit CTCGCAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTA 104 0.2332832372535385 No Hit CTCGCAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 89 0.1996366164958166 No Hit CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT 87 0.195150400394787 No Hit CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTG 85 0.19066418429375742 No Hit CTCGCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.18842107624324264 No Hit CTCGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 78 0.17496242794015388 No Hit CTCGCAGGGGACTTAACAGAGTTTTCTGACTGCGTTCTGGGCAGTGAAAATAAAGG 78 0.17496242794015388 No Hit CTCGCAGGGACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGC 78 0.17496242794015388 No Hit CTCGCAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 77 0.17271931988963907 No Hit CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 71 0.15926067158655033 No Hit CTCGCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC 70 0.15701756353603552 No Hit CTCGCAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC 69 0.15477445548552074 No Hit CTCGCAGGGGGCGCTCGCTGCGTCGACGTGCTGACGACAGGACGCCCAGGCCGGGG 68 0.15253134743500593 No Hit CTCGCAGGGATCCCCAAGGTCTTAGCCATGTCTGGAGACCCTAACTACCTGCACCG 68 0.15253134743500593 No Hit CTCGCAGGGACCTCAGGTGTCACCAGCACTTTTGACCAAGTCCTGTTACTGTGGCC 66 0.14804513133397637 No Hit CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTCTTC 65 0.14580202328346156 No Hit CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 64 0.14355891523294678 No Hit CTCGCAGGGTATGTTCATGATGTACATGGGCATGTGGATGTTTGGTTTCAAATGAA 61 0.13682959108140239 No Hit CTCGCAGGGCACCTCGCTGGGCGTTGAGCAAACTGGGAGGTATAAGCCCACCTGCT 61 0.13682959108140239 No Hit CTCGCAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAG 61 0.13682959108140239 No Hit CTCGCAGGGGGCCTCAGAATAGCATGGGCCATGGTAGTGGAGTAAAGAAAGGTCAG 59 0.1323433749803728 No Hit CTCGCAGGGGCTCCTCCTCCTCGCCCGGCCCCAGCTGCCAGCACCATGTCCGCAGG 58 0.13010026692985802 No Hit CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC 57 0.12785715887934324 No Hit CTCGCAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 57 0.12785715887934324 No Hit CTCGCAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGA 56 0.12561405082882843 No Hit CTCGCAGGGGAGGTCCTGGGGGTGTTGTTCAGCTGCAGTGCACTCTTAATTCAGGC 55 0.12337094277831363 No Hit CTCGCAGGGGGACTATGTGCCGTCAGCAGGCACCATCTCACCCACCTCTTCCCTGG 55 0.12337094277831363 No Hit CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTAC 52 0.11664161862676925 No Hit CTCGCAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGT 52 0.11664161862676925 No Hit CTCGCAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC 51 0.11439851057625444 No Hit CTCGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 0.11215540252573968 No Hit CTCGCAGGGGTCCCTCCTCCAGTAACTGCACTTGCCCCTCACAACCCACGGCAGCT 50 0.11215540252573968 No Hit CTCGCAGGGAGTAAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 0.11215540252573968 No Hit CTCGCAGGGGGCTCAGTTTGATACGGGAAGAAACCCCAAAGTATTTGGATTAGAAA 50 0.11215540252573968 No Hit CTCGCAGGGATCTTCGCCTGCGTGGTCACCCACCTGACCCTTCTTTCTCAAGGAAG 50 0.11215540252573968 No Hit CTCGCAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 49 0.10991229447522488 No Hit CTCGCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 48 0.10766918642471009 No Hit CTCGCAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC 47 0.10542607837419529 No Hit CTCGCAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGC 47 0.10542607837419529 No Hit CTCGCAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 47 0.10542607837419529 No Hit CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCT 46 0.10318297032368048 No Hit CTCGCAGGGGTGCAGTGCTCTCGCAGAAGTGAAGGCCCTCTGATGGACTTAAGTGT 46 0.10318297032368048 No Hit CTCGCAGGGATAGCATCATGGTTCTGGCCTTTTCCACATTCAATGGAAGCTTCATG 45 0.10093986227316569 No Hit CTCGCAGGGAGTCAAGAGTTTAGACTGCTCCAGCTGGGACATCCAGTCCTCGCTGC 45 0.10093986227316569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0022431080505147935 0.0 0.0 0.0 0.0 7 0.0022431080505147935 0.0 0.0 0.0 0.0 8 0.0022431080505147935 0.0 0.0 0.0 0.0 9 0.0022431080505147935 0.0 0.0 0.0 0.0 10 0.004486216101029587 0.0 0.0 0.0 0.0 11 0.004486216101029587 0.0 0.0 0.0 0.0 12 0.004486216101029587 0.0 0.0 0.0 0.0 13 0.0067293241515443795 0.0 0.0 0.0 0.0 14 0.0067293241515443795 0.0 0.0 0.0 0.0 15 0.0067293241515443795 0.0 0.0 0.0 0.0 16 0.008972432202059174 0.0 0.0 0.0 0.0 17 0.008972432202059174 0.0 0.0 0.0 0.0 18 0.008972432202059174 0.0 0.0 0.0 0.0 19 0.015701756353603553 0.0 0.0 0.0 0.0 20 0.015701756353603553 0.0 0.0 0.0 0.0 21 0.017944864404118348 0.0 0.0 0.0 0.0 22 0.022431080505147933 0.0 0.0 0.0 0.0 23 0.022431080505147933 0.0 0.0 0.0 0.0 24 0.022431080505147933 0.0 0.0 0.0 0.0 25 0.024674188555662727 0.0 0.0 0.0 0.0 26 0.024674188555662727 0.0 0.0 0.0 0.0 27 0.03140351270720711 0.0 0.0 0.0 0.0 28 0.03813283685875148 0.0 0.0 0.0 0.0 29 0.044862161010295866 0.0 0.0 0.0 0.0 30 0.04710526906081066 0.0 0.0 0.0 0.0 31 0.049348377111325455 0.0 0.0 0.0 0.0 32 0.05159148516184025 0.0 0.0 0.0 0.0 33 0.053834593212355036 0.0 0.0 0.0 0.0 34 0.053834593212355036 0.0 0.0 0.0 0.0 35 0.05607770126286983 0.0 0.0 0.0 0.0 36 0.05607770126286983 0.0 0.0 0.0 0.0 37 0.05607770126286983 0.0 0.0 0.0 0.0 38 0.06056391736389942 0.0 0.0 0.0 0.0 39 0.06280702541441421 0.0 0.0 0.0 0.0 40 0.06505013346492901 0.0 0.0 0.0 0.0 41 0.06505013346492901 0.0 0.0 0.0 0.0 42 0.06505013346492901 0.0 0.0 0.0 0.0 43 0.0672932415154438 0.0 0.0 0.0 0.0 44 0.07402256566698819 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACTTT 15 0.008072087 50.47219 48 CCTGATA 25 1.9435221E-5 50.47219 49 CTGATAG 25 1.9435221E-5 50.47219 50 GCCTGAT 25 1.9435221E-5 50.47219 48 CAAGCGC 40 2.3756002E-9 50.47219 50 GTCGGAG 20 3.9522685E-4 50.47219 48 AAGTATT 15 0.008329015 50.074326 39 GTTGAGC 20 4.1100135E-4 50.074326 41 AAGTAGC 25 2.0369383E-5 50.074326 43 TAGTGAT 15 0.008329015 50.074326 42 GCTAGTG 15 0.008329015 50.074326 40 GAAACGG 15 0.008329015 50.074326 46 ACTGAGT 15 0.008329015 50.074326 43 TTCGTCT 15 0.008329015 50.074326 36 AGGATTT 20 4.1100135E-4 50.074326 35 TAAGTAG 25 2.0369383E-5 50.074326 42 GGATTTG 15 0.008329015 50.074326 36 TGATTAC 15 0.008329015 50.074326 45 TGCTGAA 15 0.008329015 50.074326 41 CGTAGTG 15 0.008329015 50.074326 39 >>END_MODULE