FastQCFastQC Report
Fri 17 Jun 2016
SRR1527030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19236
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3691.9182782283218964No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3171.647951757122063No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.8473695154917863No Hit
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.7122062798918695No Hit
ACGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.5458515283842794No Hit
ACGTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.45227698066126015No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.37429819089207733No Hit
ACGTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.32231233104595547No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.25992929923060926No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.2495321272613849No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG400.2079434393844874No Hit
ACGTTGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.19234768143065087No Hit
ACGTTGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.18714909544603867No Hit
ACGTTGGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.15075899355375338No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.140361821584529No Hit
ACGTTGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.12996464961530463No Hit
ACGTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.11956747764608026No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG230.11956747764608026No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACGTTGGGGAAGCAGTGGTATCAA220.11436889166146808No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.11436889166146808No Hit
ACGTTGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.10917030567685589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAT201.8717833E-458.5435337
GGGAGAC150.004449171558.5435337
TGGGGTT201.8717833E-458.5435335
TGGGGTC257.897554E-658.5435335
TTGGGAT201.8717833E-458.5435334
GGGGGAT150.004449171558.5435336
GGGACGT150.004449171558.5435337
GGGGCTC150.004449171558.5435336
CGTTGAG201.8717833E-458.5435332
GGGGAGC406.039045E-1058.5435336
GGAGATG150.004590810758.083778
GGAGACG150.004590810758.083778
GGCATGC150.004590810758.083778
GGACTGT303.5329685E-758.083778
GGAAGAT150.004590810758.083778
GGAACAT150.004590810758.083778
AGTGGGT150.004638761757.93211728
ATGGTGA150.004638761757.93211716
CCATGGT150.004638761757.93211714
TCTGCTG150.004638761757.93211716
Produced by FastQC (version 0.11.5)