FastQCFastQC Report
Fri 17 Jun 2016
SRR1527029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21844
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5372.4583409631935544No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5182.3713605566746017No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2241.025453213697125No Hit
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.5768174327046328No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.44863578099249224No Hit
ACGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.42574620032960997No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG740.3387657938106574No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.3021424647500458No Hit
ACGTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.2929866324848929No Hit
ACGTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.27467496795458707No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.24720747115912836No Hit
ACGTTGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.21973997436366965No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG470.2151620582310932No Hit
ACGTTGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.19227247756821095No Hit
ACGTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.16480498077275224No Hit
ACGTTGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.16480498077275224No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.15564914850759934No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.14649331624244644No Hit
ACGTTGGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12818165171214063No Hit
ACGTTGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.12360373557956418No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC270.12360373557956418Illumina Single End Adapter 2 (96% over 31bp)
ACGTTGGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11902581944698773No Hit
ACGTTGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG250.11444790331441128No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10071415491668192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAT150.004566170758.18937348
CCGTGCG150.004566170758.18937341
TGCCGTG150.004566170758.18937339
GAGTTTG150.004566170758.18937346
TACGTAG150.004566170758.18937340
TAGAGTT150.004566170758.18937344
CGTGCGA150.004566170758.18937342
GCCGTGC150.004566170758.18937340
GTGCGAA150.004566170758.18937343
AGTTTGA150.004566170758.18937347
GTACGTA150.004566170758.18937339
TTTGATC150.004566170758.18937349
GTGTCTC150.004608208358.05529825
TGTCTCC150.004608208358.05529826
CAGTACG150.004608208358.05529837
AGTACGT150.004608208358.05529838
TCAGTAC150.004608208358.05529836
CTCAGTA150.004608208358.05529835
GTCTCCG150.004608208358.05529827
GCGTTGC150.004608208358.05529835
Produced by FastQC (version 0.11.5)