FastQCFastQC Report
Fri 17 Jun 2016
SRR1527028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21542
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5152.390678674217807No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4902.2746263113917No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2451.13731315569585No Hit
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.5709776251044472No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.41314641166094146No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.36672546653049853No Hit
ACGTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.3249466159131No Hit
ACGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.3203045214000557No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG590.27388357626961285No Hit
ACGTTGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.2553151982174357No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.22746263113916998No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG490.22746263113916998No Hit
ACGTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.21353634760003715No Hit
ACGTTGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.20889425308699286No Hit
ACGTTGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.20425215857394857No Hit
ACGTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.19496796954786No Hit
ACGTTGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.14854702441741713No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.14854702441741713No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC300.13926283539132858Illumina Single End Adapter 2 (96% over 31bp)
ACGTTGGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.13462074087828427No Hit
ACGTTGGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.12533655185219572No Hit
ACGTTGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG270.12533655185219572No Hit
ACGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.11605236282610713No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.11141026831306285No Hit
ACGTTGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG240.11141026831306285No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.11141026831306285No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.11141026831306285No Hit
ACGTTGGGTGCGGCAATACTTTGTATTGGGTAGGGGAGCGTCGTGCATGTGTGGAAGCGTTACC230.10676817380001857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACT303.3584E-758.554541
TACGTAC303.3584E-758.554540
CGTACTG303.3584E-758.554542
CTACGTA303.3584E-758.554539
GGAGACA303.470759E-758.278353
CACACCC150.004537341758.278321
AGCCAGG150.004537341758.278324
GAGCCAG150.004537341758.278323
TCGGAGA303.470759E-758.278351
GCCAGGA150.004537341758.278325
GACACGC303.470759E-758.278356
CACCCAA150.004537341758.278323
AGTCGGA303.470759E-758.278349
CCACACC150.004537341758.278320
AGACACG303.470759E-758.278355
CCAGGAT150.004537341758.278326
GAGACAC303.470759E-758.278354
GTCGGAG303.470759E-758.278350
ACTCTAC303.52813E-758.14117436
GAGTCGG303.52813E-758.14117448
Produced by FastQC (version 0.11.5)