##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21542
Sequences flagged as poor quality	0
Sequence length	64
%GC	35
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.04883483427722	39.0	38.0	40.0	35.0	40.0
2	37.78358555380188	39.0	38.0	40.0	35.0	40.0
3	37.68990808652864	39.0	38.0	40.0	35.0	40.0
4	37.322439884876054	39.0	38.0	40.0	33.0	40.0
5	37.87006777457989	39.0	38.0	40.0	35.0	40.0
6	37.43538204437842	39.0	38.0	40.0	33.0	40.0
7	37.79138427258379	39.0	38.0	40.0	35.0	40.0
8	37.66790455853681	39.0	38.0	40.0	35.0	40.0
9	37.57910129050227	39.0	38.0	40.0	34.0	40.0
10	36.95942809395599	39.0	37.0	40.0	32.0	40.0
11	37.12905022746263	39.0	37.0	40.0	33.0	40.0
12	37.00287809859809	39.0	36.0	40.0	32.0	40.0
13	36.8961563457432	39.0	36.0	40.0	32.0	40.0
14	36.91077894345929	39.0	36.0	40.0	32.0	40.0
15	36.83125986445084	39.0	36.0	40.0	32.0	40.0
16	36.53402655278062	39.0	36.0	40.0	31.0	40.0
17	36.60997121901402	39.0	36.0	40.0	31.0	40.0
18	36.45622504874199	38.0	36.0	40.0	31.0	40.0
19	36.234286510073346	38.0	35.0	40.0	31.0	40.0
20	36.36157274162102	38.0	36.0	40.0	31.0	40.0
21	36.265481385201	38.0	36.0	40.0	31.0	40.0
22	36.052734193668186	38.0	35.0	39.0	30.0	40.0
23	35.91699935010677	38.0	35.0	39.0	30.0	40.0
24	35.80856002228205	38.0	35.0	39.0	29.0	40.0
25	35.73080493918856	38.0	35.0	39.0	29.0	40.0
26	35.85962306192554	39.0	36.0	40.0	29.0	40.0
27	36.222588431900476	39.0	36.0	40.0	31.0	40.0
28	35.950329588710424	39.0	35.0	40.0	30.0	40.0
29	35.993593909572	39.0	36.0	40.0	30.0	40.0
30	35.73099062296908	39.0	35.0	40.0	29.0	40.0
31	35.778664933618046	39.0	36.0	40.0	30.0	40.0
32	35.45896388450469	39.0	35.0	40.0	29.0	40.0
33	35.62672918020611	39.0	35.0	40.0	29.0	40.0
34	35.47354006127565	39.0	35.0	40.0	29.0	40.0
35	35.20439142140934	39.0	35.0	40.0	27.0	40.0
36	34.94842632996008	39.0	35.0	40.0	26.0	40.0
37	34.86681830842076	39.0	35.0	40.0	25.0	40.0
38	34.71506823878934	39.0	35.0	40.0	24.0	40.0
39	34.63081422337759	39.0	35.0	40.0	23.0	40.0
40	34.44341286788599	39.0	35.0	39.0	23.0	40.0
41	34.14548324203881	39.0	35.0	39.0	19.0	40.0
42	34.083093491783494	39.0	35.0	39.0	18.0	40.0
43	33.91760282239346	39.0	35.0	39.0	17.0	40.0
44	33.74928047535048	39.0	34.0	39.0	17.0	40.0
45	33.24083186333674	39.0	34.0	39.0	5.0	40.0
46	32.97038343700678	39.0	33.0	39.0	4.0	40.0
47	32.97692879027017	39.0	33.0	39.0	4.0	40.0
48	32.7955157367004	39.0	33.0	39.0	4.0	40.0
49	32.56582490019497	39.0	32.0	39.0	4.0	40.0
50	32.41713861294216	38.0	32.0	39.0	4.0	40.0
51	32.029430879212704	38.0	32.0	39.0	4.0	40.0
52	31.8586482220778	38.0	32.0	39.0	4.0	40.0
53	31.620323089778108	38.0	32.0	39.0	4.0	40.0
54	31.231176306749607	37.0	31.0	39.0	4.0	40.0
55	30.810695385758056	37.0	30.0	39.0	4.0	40.0
56	30.1113174264228	37.0	29.0	39.0	4.0	40.0
57	29.750998050320305	36.0	28.0	39.0	4.0	40.0
58	29.443320025995728	36.0	26.0	39.0	4.0	40.0
59	28.98013183548417	35.0	24.0	39.0	4.0	40.0
60	28.610806796026367	35.0	21.0	39.0	4.0	40.0
61	28.126357812645065	35.0	4.0	39.0	4.0	40.0
62	27.905208430043636	35.0	4.0	39.0	4.0	40.0
63	27.413749883947638	35.0	4.0	39.0	4.0	40.0
64	27.383947637173893	35.0	4.0	39.0	4.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-15.279375579390212
1	2	-19.42043707435126
1	3	-23.358884890385458
1	4	-16.905234681826567
1	5	-21.338854450683534
1	6	-18.420653913596595
1	7	-19.758389459545906
1	8	-20.159224921322323
1	9	-19.339522204416063
1	10	-14.884293760660292
1	11	-12.640311886552169
1	12	-15.225843089839376
1	13	-12.982630559025374
1	14	-12.5661561289306
1	15	-14.053346433798204
1	16	-9.446803171498459
1	17	-11.406876338113818
1	18	-12.682484698072866
1	19	-10.6302788612396
1	20	-10.428138262998228
1	21	-11.939037206964606
1	22	-11.467636304451425
1	23	-11.91680227067842
1	24	-12.529011907324147
1	25	-11.014749444669516
1	26	-8.783749024049062
1	27	-8.711766765060485
1	28	-7.189039864937431
1	29	-9.106276814131235
1	30	-10.213931329638516
1	31	-9.057191321973928
1	32	-12.508062000482724
1	33	-12.7805955800089
1	34	-12.750046135350662
1	35	-11.827423384683641
1	36	-9.51692453414427
1	37	-11.51054944963256
1	38	-13.044025977967895
1	39	-13.114435172699359
1	40	-11.648279835435446
1	41	-9.810596979198849
1	42	-11.240556869619038
1	43	-11.967820233450354
1	44	-13.21477677101425
1	45	-11.514563458838833
1	46	-12.656782847767136
1	47	-13.122395220401703
1	48	-14.275965305439186
1	49	-12.970093254958424
1	50	-11.107473996728821
1	51	-14.084166434657163
1	52	-12.53318860708745
1	53	-11.104148897104583
1	54	-12.929806991752507
1	55	-12.048765624553603
1	56	-11.756600679018213
1	57	-10.36841757389698
1	58	-10.358544124564173
1	59	-9.596030568151821
1	60	-8.523337995954545
1	61	-9.931235545630198
1	62	-8.754728940079637
1	63	-9.285918632862646
1	64	-7.2247972258609785
2	1	1.0849101348955017
2	2	1.3724200685058818
2	3	1.4339722524716834
2	4	1.5161938896020075
2	5	0.9611455493164627
2	6	0.929346086403406
2	7	1.213039111882665
2	8	1.4122036501062496
2	9	0.9961920812982186
2	10	0.8657062393397084
2	11	0.8239738277335462
2	12	1.0527283387320523
2	13	0.5316551552603386
2	14	0.8195581567836854
2	15	0.23236785191608078
2	16	0.0031968285015437914
2	17	-0.9211620523995308
2	18	-0.5753418409300082
2	19	-0.42313600409674024
2	20	0.11471887985891271
2	21	0.24667707874967704
2	22	-0.4176363044514275
2	23	0.15462630075015227
2	24	-0.34329762160986377
2	25	-0.6076065875266607
2	26	0.2091081188080821
2	27	0.6810903777966573
2	28	0.2681029922054279
2	29	-1.041991099845518
2	30	-1.7710741867813766
2	31	-0.5786198934025037
2	32	-1.1937762861970072
2	33	-1.016309865723187
2	34	-0.12861756392209145
2	35	-0.19170909896935484
2	36	0.4902183229985866
2	37	0.9180219789388673
2	38	1.3988311648892449
2	39	0.5998505415863562
2	40	0.9517201645645557
2	41	1.33940302080115
2	42	1.830871701809535
2	43	2.1321797665496476
2	44	2.170937514700036
2	45	2.5282936840183083
2	46	2.0003600093757186
2	47	1.7061762081697225
2	48	1.7883204088465305
2	49	1.472763887898715
2	50	2.2925260032711776
2	51	3.401547851057124
2	52	3.2239542500554066
2	53	3.781565388609703
2	54	2.6773358653903507
2	55	3.072662946874967
2	56	3.493399320981787
2	57	3.460153854674445
2	58	3.2271701611501094
2	59	4.182540860419607
2	60	4.9695191469025985
2	61	4.218764454369801
2	62	5.388128202777505
2	63	3.4069385099944967
2	64	4.068059916996162
3	1	-0.4460422460568765
3	2	-0.20615136006554735
3	3	-0.7160277475283152
3	4	-0.238568015159899
3	5	-0.5531401649692498
3	6	-0.5635110564537413
3	7	-0.8298180309744794
3	8	-0.8735106356080351
3	9	-0.7919031567970194
3	10	-0.6938175701841018
3	11	0.026354780114495213
3	12	-1.0829859469822338
3	13	-0.9112019875968045
3	14	0.21955815678368396
3	15	-0.2914416718934447
3	16	-1.1610888857841744
3	17	-1.144971576209059
3	18	0.007991492403327527
3	19	-1.0350407660015009
3	20	0.09567126081129373
3	21	-1.2485610164884164
3	22	-1.2057315425466655
3	23	-1.9406117944879426
3	24	-1.4099642882765266
3	25	-1.1814161113361834
3	26	1.073393833093796
3	27	-0.30700486029858354
3	28	0.5490553731578061
3	29	-0.7491339569883735
3	30	-0.4282170439242279
3	31	-0.1048103695929754
3	32	0.03955704713632713
3	33	0.243213943800626
3	34	0.7737633884588604
3	35	0.7678147105544539
3	36	1.7211707039509676
3	37	1.0370695979864877
3	38	1.217878783936868
3	39	1.885564827300641
3	40	1.7564820693264593
3	41	1.8084506398487719
3	42	0.8070621780000096
3	43	1.5321797665496462
3	44	1.8090327527952752
3	45	1.7473413030659302
3	46	0.628931437947152
3	47	0.4966523986459137
3	48	0.7240346945608138
3	49	1.0537162688510975
3	50	0.9877640985092739
3	51	0.9158335653428367
3	52	0.8477637738649335
3	53	1.2053749124192272
3	54	1.0701930082474966
3	55	1.9036153278273495
3	56	-0.47088639330392823
3	57	-0.1065128119922214
3	58	-0.6442584102784608
3	59	-1.1436496157708689
3	60	-0.5233379959545452
3	61	0.021145406750754603
3	62	-0.32615751150821026
3	63	-0.0954424423864566
3	64	0.10853610747235365
4	1	0.9825291825145541
4	2	1.3176581637439782
4	3	0.9030198715193052
4	4	1.3804796038877214
4	5	1.2563836445545604
4	6	0.8650603721176893
4	7	0.9320867309302869
4	8	0.983632221534819
4	9	1.0652397003458418
4	10	-0.9795318558983865
4	11	-2.735549981790264
4	12	-1.6544145184108032
4	13	-1.244535320930133
4	14	-0.8756799384544109
4	15	-1.1962035766553498
4	16	-0.35156507626036415
4	17	0.09312366188618171
4	18	0.007991492403327527
4	19	0.5840068530461124
4	20	0.38138554652557843
4	21	1.227629459702058
4	22	0.03236369554857532
4	23	0.44034058646443697
4	24	0.5900357117234734
4	25	1.294774364854291
4	26	1.2638700235699858
4	27	0.16918561589189807
4	28	1.1681029922054265
4	29	0.727056519202101
4	30	0.23844962274243642
4	31	-1.58100084578345
4	32	-0.7699667623874831
4	33	-1.5663098657231842
4	34	-0.9881413734458988
4	35	-2.0417090989693563
4	36	-0.707400724620463
4	37	-0.296263735346848
4	38	-1.1630735970155115
4	39	-0.9715780298422132
4	40	-1.1482798354354458
4	41	-1.5248826934845638
4	42	-0.33579496485712923
4	43	-1.324963090593208
4	44	-0.8100148662523452
4	45	-0.34789679217216474
4	46	-1.0853542763385633
4	47	-1.789061887068371
4	48	-1.466441495915376
4	49	0.14895436408919238
4	50	-0.536045425300248
4	51	-3.3698807203714516
4	52	-2.580807654706497
4	53	-2.8422441351998238
4	54	-2.5964736584191748
4	55	-4.28686086264884
4	56	-5.328029250446786
4	57	-4.5350842405636485
4	58	-4.263306029326078
4	59	-3.953173425294679
4	60	-3.8566713292878774
4	61	-3.0740926884873403
4	62	-3.3737765591272577
4	63	-3.476394823338836
4	64	-3.367654368718121
5	1	0.28491013489550454
5	2	0.37242006850588183
5	3	0.3839722524716862
5	4	0.36619388960200894
5	5	0.4111455493164655
5	6	0.7793460864034074
5	7	1.0630391118826665
5	8	1.262203650106251
5	9	1.2961920812982228
5	10	1.015706239339707
5	11	0.6739738277335476
5	12	0.5527283387320523
5	13	0.43165515526034426
5	14	0.36955815678368253
5	15	1.532367851916078
5	16	1.353196828501538
5	17	0.7288379476004678
5	18	1.4246581590699918
5	19	1.8268639959032598
5	20	0.414718879858917
5	21	0.24667707874967704
5	22	0.7323636955485782
5	23	1.3046263007501508
5	24	-0.24329762160986235
5	25	0.5423934124733449
5	26	-0.1408918811919193
5	27	-0.4689096222033413
5	28	-0.5318970077945764
5	29	0.008008900154479193
5	30	0.37892581321862906
5	31	0.1213801065974991
5	32	0.9062237138029943
5	33	0.5336901342768172
5	34	1.0213824360779142
5	35	0.6582909010306466
5	36	1.8902183229985852
5	37	1.8680219789388701
5	38	1.7988311648892505
5	39	1.799850541586359
5	40	2.6017201645645542
5	41	2.8894030208011543
5	42	2.3808717018095322
5	43	2.4321797665496447
5	44	2.0209375147000372
5	45	3.478293684018311
5	46	3.050360009375723
5	47	2.806176208169724
5	48	3.388320408846532
5	49	2.7727638878987193
5	50	1.5425260032711776
5	51	2.151547851057124
5	52	2.623954250055405
5	53	2.8315653886097003
5	54	2.777335865390352
5	55	3.722662946874973
5	56	3.743399320981787
5	57	2.360153854674447
5	58	3.0771701611501108
5	59	0.9825408604196042
5	60	1.8695191469025971
5	61	0.46876445436980063
5	62	1.5881282027775043
5	63	-0.7430614900055055
5	64	-1.2819400830038354
6	1	0.40859434542181816
6	2	0.11715691061114342
6	3	-0.24234353700200018
6	4	-0.5969640051348364
6	5	0.1085139703690956
6	6	-0.3153907557018556
6	7	0.2077759539879267
6	8	0.30694049221150976
6	9	0.3409289234034816
6	10	-0.20008323434450404
6	11	-0.13655248805592635
6	12	-0.5341137665311066
6	13	-0.1893974763186037
6	14	-0.043599737953158524
6	15	0.49552574665292326
6	16	0.02688103902785599
6	17	0.3262063686530965
6	18	0.8325528959120945
6	19	0.2531797853769433
6	20	0.8726136167010168
6	21	1.4256244471707333
6	22	1.9797321166012054
6	23	2.1546263007501523
6	24	1.62512343102172
6	25	0.7634460440522872
6	26	1.2538449609133409
6	27	1.4047745883229723
6	28	0.2733661501001663
6	29	1.371166794891323
6	30	1.4815573921659961
6	31	0.424011685544869
6	32	0.7588552927503613
6	33	-0.14525723414423908
6	34	-2.1259859849747187
6	35	0.22144879576748622
6	36	0.2875867440512181
6	37	1.4180219789388673
6	38	1.1251469543629327
6	39	0.12616633106004116
6	40	-0.8324903617512334
6	41	0.399929336590624
6	42	0.5413980175990076
6	43	0.637442924444386
6	44	0.8340954094368769
6	45	0.023030526123569928
6	46	0.7266757988494064
6	47	0.09564989238025134
6	48	0.2804256720044265
6	49	0.8306586247408205
6	50	0.892526003271179
6	51	1.412074166846594
6	52	1.0081647763711956
6	53	1.7341969675570716
6	54	1.822072707495611
6	55	2.41739978898023
6	56	2.5591887946659995
6	57	3.0338380652007615
6	58	2.4008543716764272
6	59	3.543067176209078
6	60	3.4089928311131246
6	61	3.2266591912119083
6	62	2.0197071501459263
6	63	2.6464121942050234
6	64	3.1812178117330063
7	1	0.9349101348955031
7	2	1.2750516474532532
7	3	1.0734459366822122
7	4	1.245141258023061
7	5	1.2137771282638354
7	6	1.421451349561302
7	7	1.3130391118826665
7	8	1.0964141764220372
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116	26	0.3591081188080807
116	27	0.021999468705750758
116	28	-1.5137151896127534
116	29	-0.2101729180273395
116	30	-1.389256004963194
116	31	0.23501647023386596
116	32	-0.20286719528791508
116	33	0.6155083160949957
116	34	-0.451344836649362
116	35	0.27647271921246386
116	36	0.7493092320894945
116	37	0.00893106984796077
116	38	0.9624675285256146
116	39	-0.4001494584136438
116	40	1.1244474372918276
116	41	2.234857566255698
116	42	1.9217807927186286
116	43	2.532179766549646
116	44	1.661846605609128
116	45	2.94647550220013
116	46	3.2003600093757214
116	47	2.806176208169724
116	48	3.3474113179374356
116	49	2.809127524262351
116	50	2.756162366907546
116	51	2.7015478510571214
116	52	2.5966815227826814
116	53	2.8633835704278816
116	54	3.245517683572171
116	55	3.977208401420423
116	56	2.6979447755272403
116	57	2.787426581947173
116	58	3.0635337975137453
116	59	-0.6220045941258476
116	60	-0.8350263076428561
116	61	-0.931235545630198
116	62	-1.702780888131585
116	63	-0.8703342172782307
116	64	-2.3092128102765628
117	1	-0.32824775984133936
117	2	-0.14600098412569906
117	3	0.2839722524716848
117	4	0.19250967907569105
117	5	-0.5230649769993221
117	6	0.21092503377182226
117	7	-0.002750361801545864
117	8	0.20167733431677703
117	9	0.4461920812982214
117	10	-0.042188497502394284
117	11	1.1266054066809161
117	12	0.9395704439952084
117	13	1.1263919973656016
117	14	0.009031840994211393
117	15	0.8639467992844985
117	16	-0.39417159255108913
117	17	-0.20010942082058847
117	18	-0.27271026198263826
117	19	-0.3783991619914815
117	20	0.13577151143785926
117	21	1.2150981313812608
117	22	0.9797321166012054
117	23	1.1546263007501523
117	24	1.2040707994427677
117	25	0.3423934124733421
117	26	0.8854239082817656
117	27	1.036353535691397
117	28	0.48389246588963886
117	29	0.7922194264702682
117	30	0.9552416026923112
117	31	2.897695896071184
117	32	2.7062237138029914
117	33	1.9600059237505008
117	34	2.9266455939726512
117	35	2.4846066905043287
117	36	2.9717972703670057
117	37	3.049600926307285
117	38	2.2830416912050353
117	39	2.4945873836916164
117	40	2.2727727961434994
117	41	2.6104556523800966
117	42	2.4887664386516377
117	43	2.8479692402338586
117	44	1.9919901462789795
117	45	2.7598726313867274
117	46	3.516149483059934
117	47	3.358807787117094
117	48	3.543583566741269
117	49	3.9359217826355604
117	50	3.945157582218549
117	51	1.9910215352676488
117	52	1.6397437237396204
117	53	2.944723283346544
117	54	3.348388496969296
117	55	3.3647682100328673
117	56	2.9276098472975747
117	57	2.9812064862533916
117	58	2.8745385822027423
117	59	4.069382965682763
117	60	4.408992831113125
117	61	4.437185507001384
117	62	4.124970308040663
117	63	4.277991141573445
117	64	4.918059916996164
118	1	0.25309195307732324
118	2	-0.004852658766843376
118	3	-0.03420956571013534
118	4	0.43892116232927947
118	5	0.20660009477101227
118	6	0.3975279045852247
118	7	0.04031183915539316
118	8	0.00311274101534309
118	9	-0.008353373247231843
118	10	-0.9752028515693851
118	11	-1.2896625359028207
118	12	-1.3427262067224959
118	13	-1.359253935648752
118	14	-2.598623661398136
118	15	-1.0858139662657393
118	16	-2.810439535134826
118	17	-1.53025296149044
118	18	-1.4162509318391017
118	19	-2.227681458642195
118	20	-1.921644756504719
118	21	-1.1078683757957748
118	22	-0.740363577178698
118	23	-1.118100971977121
118	24	-1.1069339852462292
118	25	-1.3394247693448378
118	26	-1.368164608464646
118	27	-3.2961823494760694
118	28	-1.9682606441582067
118	29	-1.8919910998455194
118	30	-3.389256004963194
118	31	-3.128619893402501
118	32	-4.793776286197009
118	33	-4.611764411177731
118	34	-4.314981200285725
118	35	-5.087163644423903
118	36	-4.387054404274139
118	37	-4.991068930152043
118	38	-5.764805198747116
118	39	-4.5365130947772805
118	40	-4.830098017253629
118	41	-3.3560515246533953
118	42	-2.9873101163722815
118	43	-2.831456597086717
118	44	-3.110880667118149
118	45	-2.417160861436237
118	46	-3.0269127178970052
118	47	-2.9665510645575495
118	48	-2.152588682062561
118	49	-2.418145203010372
118	50	-2.016564905819731
118	51	-1.4348157853065153
118	52	-1.4942275681264086
118	53	-1.2275255204812083
118	54	-0.34539140733692264
118	55	-0.56824614403412
118	56	1.3797629573454238
118	57	1.378335672856263
118	58	2.1998974338773856
118	59	2.0598135876923322
118	60	0.21042823781168707
118	61	-0.4766900910847447
118	62	-0.020962706313405022
118	63	-0.09760694455095731
118	64	0.23624173517798042
119	1	0.32379902378438885
119	2	0.38908673517254755
119	3	0.3395278080272419
119	4	0.7217494451575632
119	5	0.883367771538687
119	6	0.7460127530700689
119	7	1.035261334104888
119	8	1.134425872328471
119	9	1.1128587479648857
119	10	1.3379284615619298
119	11	0.962862716622432
119	12	1.1471727831764937
119	13	-0.3239004002952157
119	14	-0.5026640654385375
119	15	-1.4898543703061407
119	16	-0.8912476159429019
119	17	-0.6211620523995336
119	18	-1.0475640631522296
119	19	-0.8286915596522988
119	20	-1.2297255645855287
119	21	-0.5977673656947644
119	22	-1.3009696377847604
119	23	-1.512040365916512
119	24	-1.076630954943198
119	25	-0.8242732541933222
119	26	-2.474225214525255
119	27	-3.180020733314457
119	28	-3.443008118905688
119	29	-3.169768877623298
119	30	-3.071074186781374
119	31	-2.8508421156247223
119	32	-2.404887397308123
119	33	-2.7329765323898485
119	34	-4.511950897255421
119	35	-3.986153543413799
119	36	-3.604226121445855
119	37	-4.693089132172243
119	38	-4.901168835110752
119	39	-3.455705013969201
119	40	-2.981613168768778
119	41	-3.1439303125321807
119	42	-4.169128298190465
119	43	-3.801153566783686
119	44	-3.5401735964110763
119	45	-2.5939285382039117
119	46	-2.5218622128465
119	47	-2.860490458496944
119	48	-2.2839018133756923
119	49	-3.49390277876795
119	50	-2.440807330062153
119	51	-2.576229926720657
119	52	-2.620490194389035
119	53	-2.2073235002791876
119	54	-1.8504419123874278
119	55	-1.421781497569473
119	56	-1.2010451234626558
119	57	-1.3842905897699964
119	58	-0.9617187277387771
119	59	-3.778570250691505
119	60	-1.9915919642085136
119	61	-2.986791101185755
119	62	-2.667427352778052
119	63	-3.2541726011166148
119	64	-2.193051194114947
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	69.0
5	2.0
6	13.0
7	13.0
8	56.0
9	46.0
10	56.0
11	43.0
12	48.0
13	71.0
14	80.0
15	77.0
16	109.0
17	140.0
18	110.0
19	147.0
20	155.0
21	193.0
22	235.0
23	212.0
24	190.0
25	238.0
26	234.0
27	313.0
28	393.0
29	433.0
30	539.0
31	695.0
32	791.0
33	1010.0
34	1208.0
35	1367.0
36	1692.0
37	2468.0
38	4355.0
39	3718.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.27105580833874	0.445723836939363	0.08357321942613055	0.19964713529575634
8	98.61007809594645	0.8320937151357382	0.24172554853105244	0.31610264038676084
9	93.15755268777272	4.252158573948566	1.4576176770959057	1.1326710611828057
10	27.06341101104818	49.03908643579983	9.516293751740786	14.381208801411196
11	21.720360226534215	42.6515643858509	20.063132485377402	15.564942902237489
12	45.30684244731223	24.714511187447776	15.662426887011419	14.316219478228575
13	21.896759818029896	22.774115680995266	27.51369417881348	27.81543032216136
14	27.476557422709124	34.67180391792777	20.151332281125246	17.700306378237862
15	37.9351963605979	26.719896017082906	18.46625197289017	16.87865564942902
16	19.970290595116516	30.874570606257546	28.01968248073531	21.135456317890633
17	30.623897502553156	28.04289295330053	18.526599201559744	22.806610342586573
18	28.21929254479621	32.076873085136015	20.675888961099247	19.027945408968527
19	21.093677467273235	31.157738371553243	27.65295701420481	20.095627146968713
20	18.925819329681552	37.36421873549345	18.689072509516294	25.020889425308702
21	19.496796954786	29.16627982545725	27.67616748677003	23.660755732986726
22	22.68127379073438	31.241296072788042	18.800482777829355	27.276947358648222
23	25.41782729805014	36.19777158774373	21.49489322191272	16.889507892293405
24	22.1381487327082	39.597066196267754	22.175285488812552	16.089499582211495
25	22.16650415563913	26.716812926591448	27.35292752008172	23.7637553976877
26	27.75198161437081	25.284930350358803	26.931194596876317	20.03189343839407
27	21.221835075493612	25.3054587688734	28.069686411149824	25.403019744483156
28	20.092936802973977	34.00557620817844	30.153345724907066	15.748141263940521
29	26.32312624878026	26.225547140002785	31.378653408298874	16.072673202918082
30	17.600855177542293	36.02435396913924	34.081613682840675	12.293177170477785
31	19.704296075878744	31.871861632880787	37.251255346847685	11.172586944392785
32	12.093585748174334	30.33629471138192	47.10916786827294	10.460951672170799
33	14.018517656911552	30.9449588238031	40.96217373098218	14.07434978830317
34	14.883720930232558	32.43255813953488	42.753488372093024	9.930232558139535
35	11.791267107345686	37.72460664742575	41.31831300623778	9.165813238990783
36	11.510891826475516	31.84230124743996	43.93502141128282	12.711785514801713
37	11.405959031657357	30.507448789571693	48.08659217877095	10.0
38	10.750535431604433	31.74411025235124	48.230747741875405	9.274606574168917
39	10.895376449224752	31.09838431810774	48.22368114727383	9.782558085393676
40	11.188746040618595	30.897149245388484	48.84013415315819	9.07397056083473
41	9.717561521252795	32.89988814317674	47.32941834451901	10.053131991051455
42	10.65218404736376	31.131415784811896	46.990816278961354	11.22558388886299
43	12.659113162680095	30.880775866088495	47.53578589080058	8.92432508043083
44	10.529504987414933	31.411391815046148	49.46396942295143	8.59513377458749
45	11.343353687177078	32.13715359323626	47.43541568811649	9.084077031470175
46	11.45721613429704	32.09605968757286	46.691536488692	9.755187689438097
47	11.412587412587413	32.07925407925408	47.94405594405595	8.564102564102564
48	11.86978219299473	32.89492094585141	47.00806865351429	8.227228207639568
49	11.305970149253731	34.17444029850746	45.8955223880597	8.624067164179104
50	9.817554010545471	34.837385096355746	46.703373617656666	8.641687275442116
51	9.271275850800615	34.718267121049436	47.402082068997714	8.608374959152233
52	9.758147352693996	34.69978522737884	46.316182650107386	9.225884769819778
53	10.95040358325946	35.095413614519664	45.3086362151822	8.645546587038679
54	10.798450697652713	34.70063932054692	45.307760511456436	9.193149470343927
55	10.196938585028933	35.79895463879037	44.614523053948105	9.389583722232594
56	11.22777544449111	35.815950347659715	43.79112417751645	9.165150030332727
57	10.59608831629557	36.75022172431499	44.1114689819353	8.542220977454138
58	10.993371300532164	37.08337223415181	42.51237046027448	9.410886005041547
59	10.839209825806753	37.62200532386868	43.025265025918834	8.513519824405735
60	11.372658913642521	36.67740880855635	41.82429592265658	10.125636355144552
61	11.157294974780498	37.11937231458995	41.81300205492247	9.910330655707082
62	11.037929745889388	37.93908819133034	40.97533632286996	10.047645739910314
63	11.411748212533295	39.053226786298424	39.810271508014395	9.724753493153885
64	12.309632813229936	38.74147435298514	38.88629356255255	10.062599271232365
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	2.5
6	4.0
7	55.0
8	106.0
9	358.0
10	401.5
11	193.0
12	143.0
13	105.0
14	117.0
15	223.5
16	375.5
17	421.0
18	444.5
19	468.0
20	554.0
21	826.0
22	1012.0
23	1115.0
24	1340.0
25	1462.0
26	1451.5
27	1441.0
28	1441.0
29	1214.0
30	1104.0
31	1221.0
32	947.5
33	674.0
34	610.5
35	505.5
36	464.0
37	466.5
38	458.5
39	448.0
40	419.0
41	376.5
42	363.0
43	356.5
44	350.0
45	397.5
46	435.5
47	426.0
48	413.0
49	367.5
50	335.0
51	386.5
52	410.0
53	382.0
54	530.0
55	669.0
56	660.0
57	567.0
58	474.0
59	445.5
60	413.5
61	410.0
62	341.5
63	250.5
64	228.0
65	187.5
66	125.0
67	103.0
68	86.0
69	69.0
70	64.0
71	53.5
72	48.0
73	38.5
74	24.0
75	19.0
76	15.0
77	10.0
78	9.0
79	6.0
80	2.0
81	1.0
82	2.0
83	3.0
84	2.0
85	0.5
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.01856837805217714
8	0.13926283539132858
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.00928418902608857
24	0.0
25	0.023210472565221428
26	1.0259028873827871
27	0.07891560672175287
28	0.10212607928697429
29	0.09748398477393
30	0.12069445733915143
31	0.15783121344350573
32	0.19961006406090426
33	0.22746263113916998
34	0.19496796954786
35	0.27852567078265716
36	0.26924148175656853
37	0.28780985980874574
38	0.29709404883483426
39	0.30173614334787857
40	0.3435149939652771
41	0.3992201281218085
42	0.42243060068703
43	0.4409989787392071
44	0.40850431714789714
45	1.16980781728716
46	0.4502831677652957
47	0.4270726952000743
48	0.4688515458174728
49	0.4734936403305171
50	0.5152724909479157
51	0.5616934360783585
52	0.5756197196174915
53	0.5059883019218271
54	0.5245566799740042
55	0.5291987744870486
56	0.5245566799740042
57	0.55240924705227
58	0.5570513415653143
59	0.5988301921827128
60	0.6081143812088013
61	0.6034722866957571
62	0.6220406647479343
63	0.6638195153653328
64	0.6313248537740229
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
64	21542.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.373131556958505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71409121851599	48.92303407297373
2	11.17162090613418	13.71274719153282
3	3.872626881476439	7.130257172036022
4	1.762347780046895	4.326432086157274
5	1.0589214129037139	3.2494661591310003
6	0.6277891233643447	2.311763067496054
7	0.4386960139172528	1.8846903722959798
8	0.3252401482489978	1.596880512487234
9	0.2193480069586264	1.2115866679045584
>10	0.733681264654716	7.325225141583883
>50	0.04538234626730202	1.954321789991644
>100	0.02269117313365101	3.9829170921919967
>500	0.00756372437788367	2.390678674217807
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	515	2.390678674217807	No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	490	2.2746263113917	No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	245	1.13731315569585	No Hit
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.5709776251044472	No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.41314641166094146	No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79	0.36672546653049853	No Hit
ACGTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.3249466159131	No Hit
ACGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69	0.3203045214000557	No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG	59	0.27388357626961285	No Hit
ACGTTGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.2553151982174357	No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49	0.22746263113916998	No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG	49	0.22746263113916998	No Hit
ACGTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46	0.21353634760003715	No Hit
ACGTTGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.20889425308699286	No Hit
ACGTTGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44	0.20425215857394857	No Hit
ACGTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42	0.19496796954786	No Hit
ACGTTGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.14854702441741713	No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.14854702441741713	No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC	30	0.13926283539132858	Illumina Single End Adapter 2 (96% over 31bp)
ACGTTGGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29	0.13462074087828427	No Hit
ACGTTGGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.12533655185219572	No Hit
ACGTTGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG	27	0.12533655185219572	No Hit
ACGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25	0.11605236282610713	No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24	0.11141026831306285	No Hit
ACGTTGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG	24	0.11141026831306285	No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24	0.11141026831306285	No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24	0.11141026831306285	No Hit
ACGTTGGGTGCGGCAATACTTTGTATTGGGTAGGGGAGCGTCGTGCATGTGTGGAAGCGTTACC	23	0.10676817380001857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.004642094513044285	0.0	0.0	0.0	0.0
8	0.004642094513044285	0.0	0.0	0.0	0.0
9	0.00928418902608857	0.0	0.0	0.0	0.0
10	0.027852567078265715	0.0	0.0	0.0	0.0
11	0.07427351220870856	0.0	0.0	0.0	0.0
12	0.07891560672175285	0.0	0.0	0.0	0.0
13	0.08355770123479714	0.0	0.0	0.0	0.0
14	0.11605236282610713	0.0	0.0	0.0	0.0
15	0.11605236282610713	0.0	0.0	0.0	0.0
16	0.12533655185219572	0.0	0.0	0.0	0.0
17	0.12997864636524	0.0	0.0	0.0	0.0
18	0.13926283539132855	0.0	0.0	0.0	0.0
19	0.15318911893046142	0.0	0.0	0.0	0.0
20	0.20889425308699286	0.0	0.0	0.0	0.0
21	0.20889425308699286	0.0	0.0	0.0	0.0
22	0.23674682016525855	0.0	0.0	0.0	0.0
23	0.29245195432179	0.0	0.0	0.0	0.0
24	0.3249466159131	0.0	0.0	0.0	0.0
25	0.3435149939652771	0.0	0.0	0.0	0.0
26	0.36672546653049853	0.0	0.0	0.0	0.0
27	0.3852938445826757	0.0	0.0	0.0	0.0
28	0.5059883019218271	0.0	0.0	0.0	0.0
29	0.5291987744870486	0.0	0.0	0.0	0.0
30	0.5756197196174914	0.0	0.0	0.0	0.0
31	0.58490390864358	0.0	0.0	0.0	0.0
32	0.58490390864358	0.0	0.0	0.0	0.0
33	0.6034722866957571	0.0	0.0	0.0	0.0
34	0.61739857023489	0.0	0.0	0.0	0.0
35	0.6684616098783771	0.0	0.0	0.0	0.0
36	0.6777457989044657	0.0	0.0	0.0	0.0
37	0.6870299879305543	0.0	0.0	0.0	0.0
38	0.7102404604957757	0.0	0.0	0.0	0.0
39	0.74737721660013	0.0	0.0	0.0	0.0
40	0.7752297836783957	0.0	0.0	0.0	0.0
41	0.8030823507566615	0.0	0.0	0.0	0.0
42	0.8216507288088385	0.0	0.0	0.0	0.0
43	0.8634295794262371	0.0	0.0	0.0	0.0
44	0.8866400519914586	0.0	0.0	0.0	0.0
45	0.9191347135827685	0.0	0.0	0.0	0.0
46	0.9284189026088571	0.0	0.0	0.0	0.0
47	0.9469872806610342	0.0	0.0	0.0	0.0
48	0.9655556587132114	0.0	0.0	0.0	0.0
49	0.9841240367653885	0.0	0.0	0.0	0.0
50	1.0026924148175658	0.0	0.0	0.0	0.0
51	1.0073345093306099	0.0	0.0	0.0	0.0
52	1.0212607928697428	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTACT	30	3.3584E-7	58.5545	41
TACGTAC	30	3.3584E-7	58.5545	40
CGTACTG	30	3.3584E-7	58.5545	42
CTACGTA	30	3.3584E-7	58.5545	39
GGAGACA	30	3.470759E-7	58.2783	53
CACACCC	15	0.0045373417	58.2783	21
AGCCAGG	15	0.0045373417	58.2783	24
GAGCCAG	15	0.0045373417	58.2783	23
TCGGAGA	30	3.470759E-7	58.2783	51
GCCAGGA	15	0.0045373417	58.2783	25
GACACGC	30	3.470759E-7	58.2783	56
CACCCAA	15	0.0045373417	58.2783	23
AGTCGGA	30	3.470759E-7	58.2783	49
CCACACC	15	0.0045373417	58.2783	20
AGACACG	30	3.470759E-7	58.2783	55
CCAGGAT	15	0.0045373417	58.2783	26
GAGACAC	30	3.470759E-7	58.2783	54
GTCGGAG	30	3.470759E-7	58.2783	50
ACTCTAC	30	3.52813E-7	58.141174	36
GAGTCGG	30	3.52813E-7	58.141174	48
>>END_MODULE