FastQCFastQC Report
Fri 17 Jun 2016
SRR1527027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18305
Sequences flagged as poor quality0
Sequence length56
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5833.1849221524173723No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3842.097787489756897No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2641.4422288992078667No Hit
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.6009287080032778No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.45342802512974595No Hit
ACGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.4479650368751707No Hit
ACGTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.4479650368751707No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG650.3550942365473914No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA580.31685331876536466No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.30592734225621415No Hit
ACGTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.28407538923791315No Hit
ACGTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG430.23490849494673585No Hit
ACGTTGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.23490849494673585No Hit
ACGTTGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.22398251843758535No Hit
ACGTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.21305654192843487No Hit
ACGTTGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.18027861240098333No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.18027861240098333No Hit
ACGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT300.16388964763725758No Hit
ACGTTGGGTGCGGCAATACTTTGTATTGGGTAGGGGAGCGTCGTGCATGTGTGGAA300.16388964763725758No Hit
ACGTTGGGGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.14203769461895657No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.13111171810980607No Hit
ACGTTGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA240.13111171810980607No Hit
ACGTTGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12018574160065555No Hit
ACGTTGGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.12018574160065555No Hit
ACGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12018574160065555No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG210.11472275334608031Illumina Single End Adapter 2 (95% over 23bp)
ACGTTGGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.10925976509150505No Hit
ACGTTGGGGAATGGTCAACGCAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1037967768369298No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1037967768369298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGAGG150.00827576650.03571326
TGCGTGT150.00836636449.89863222
CCTGCGT150.00836636449.89863220
GCGTGTC150.00836636449.89863223
CTGCGTG150.00836636449.89863221
ACAGTGT150.00845769849.76229510
GATGGAA150.00845769849.7622959
GGATGGA150.00845769849.7622958
GGGTCGA150.00845769849.7622958
TCCCAGA150.00845769849.76229513
ATCTTGA252.064689E-549.76229510
ATCTCAT204.1730382E-449.76229512
GAAGCTG204.1730382E-449.7622959
AAGAGTG150.00845769849.76229510
CATCCCT150.00845769849.76229516
CATCTCA204.1730382E-449.76229511
AGTGGCA150.00845769849.76229513
TGATATC150.00845769849.76229516
GAAGAGT150.00845769849.7622959
ATGGAAA150.00845769849.76229510
Produced by FastQC (version 0.11.5)