FastQCFastQC Report
Fri 17 Jun 2016
SRR1527026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161799
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.42089258895296017No Hit
TCTACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.1304087169883621No Hit
TCTACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.11124914245452691No Hit
TCTACCGGTCATAGAGCTGAATCACATTAAAAAGTGCAATACAGTTCGAGGCGTCTTTGTCCTG1670.10321448216614443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTC150.00464867358.0982351
TAGACAA150.004665866558.044141
ACCGATC150.004665866558.044142
TAGATAT202.0099443E-457.9720836
GGCATAC150.00469462757.9541138
GGATAGT258.676589E-657.954118
CGTAATC202.0223076E-457.9002435
AGCGTAA202.0254079E-457.88231333
GAGCGTA202.0254079E-457.88231332
GCGTAAT202.0254079E-457.88231334
CAGGTAT202.028512E-457.864415
CTAGAGT150.004723519657.864412
GTACCAA150.004723519657.864414
TCTAGTC202.028512E-457.864414
TCAGGTA202.028512E-457.864414
TACTATC150.004723519657.864415
TGAGGTA150.004723519657.864428
GCAATAT150.004723519657.864424
TATACCG150.004723519657.864428
TAATGTG150.004723519657.864415
Produced by FastQC (version 0.11.5)