FastQCFastQC Report
Fri 17 Jun 2016
SRR1527025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226762
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.4850900944602711No Hit
TCTACCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT6740.297227930605657No Hit
TCTACCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC6630.2923770296610543No Hit
TCTACCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT4510.19888693872871116No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4320.1905081098243974No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4170.18389324489993913No Hit
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4040.17816036196540866No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG3760.16581261410641993No Hit
TCTACCGGGACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTA3310.14596801933304523No Hit
TCTACCGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTT3050.13450225346398426No Hit
TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2940.12965135251938156No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2900.12788738853952603No Hit
TCTACCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2860.1261234245596705No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2800.1234774785898872No Hit
TCTACCGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA2750.12127252361506778No Hit
TCTACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.12127252361506778No Hit
TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2620.11553964068053729No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC2620.11553964068053729No Hit
TCTACCGGTCATAGAGCTGAATCACATTAAAAAGTGCAATACAGTTCGAGGCGTCTTTGTCCTG2600.11465765869060954No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT2540.11201171272082625No Hit
TCTACCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2540.11201171272082625No Hit
TCTACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGC2400.10583783879133189No Hit
TCTACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC2400.10583783879133189No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2310.10186891983665694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA150.004622364458.18922456
TTATCTG150.004622364458.18922457
TCCGATG150.004626426458.17631554
CCAACTA150.00463049158.16341855
TAGTCAA150.004646776258.11187744
ATTACTA150.004654934658.0861439
ACGAGCG150.004654934658.0861439
ACGCCTA150.00465901858.0732838
TTAATAC150.004671283558.03473734
ATCTCAA150.004675377658.021930
CGATGCG150.0046917857.97060827
CGTCCTA303.76127E-757.97060827
GGACTAA303.7843165E-757.919418
CGGGATA2300.057.919416
TACCGTA150.004708225457.919413
GACTAGC150.004708225457.9194125
CGGGTTA407.239578E-1057.9194076
CGAAATA150.00471234357.90662422
AACGCTC150.00471234357.90662423
GAGCATA150.00471234357.90662422
Produced by FastQC (version 0.11.5)