FastQCFastQC Report
Fri 17 Jun 2016
SRR1527024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227280
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.43338613164378736No Hit
TCTACCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC6820.30007039774727207No Hit
TCTACCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT6260.27543118620204154No Hit
TCTACCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT4350.19139387539598732No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4250.18699401619148187No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4250.18699401619148187No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG3660.1610348468848997No Hit
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3420.15047518479408659No Hit
TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3060.13463569165786696No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2960.1302358324533615No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2960.1302358324533615No Hit
TCTACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.12495600140795494No Hit
TCTACCGGGACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTA2760.12143611404435058No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC2740.1205561422034495No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT2650.11659626891939458No Hit
TCTACCGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA2590.11395635339669132No Hit
TCTACCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2570.11307638155579021No Hit
TCTACCGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTTC2450.10779655051038367No Hit
TCTACCGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTT2440.10735656458993313No Hit
TCTACCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2440.10735656458993313No Hit
TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2300.10119676170362549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC150.004531312358.48227744
TTACTAG150.00453531558.4692440
CGACAAA150.00453531558.4692441
AGATTCG258.2437255E-658.4692442
CCGACAA150.00453531558.4692440
ACGTAGA303.544992E-758.4692441
AAATCAT258.33129E-658.36515825
CGAAATA150.00457145758.3521722
ACGAAAT150.00457548658.3391921
TAATTGG258.609912E-658.04226756
TAATCAG150.00467707258.0165950
CCATGAC150.00468527157.99093638
AGTAAGT202.0084552E-457.99093636
GACGCAT150.004714051357.9013232
ATCATAT202.0327244E-457.85024327
ACCGGAT950.057.748354
CGGCATG150.004763694557.748356
GGATACC150.004763694557.748358
CGGGATA1600.057.748356
ATTCGTG202.0549892E-457.72293518
Produced by FastQC (version 0.11.5)