FastQCFastQC Report
Fri 17 Jun 2016
SRR1527023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207609
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11450.5515175160999763No Hit
TCTACCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG6400.30827179939212657No Hit
TCTACCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC6180.2976749562880222No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5750.2769629447663637No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5170.24902581294645224No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4150.19989499491833207No Hit
TCTACCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3780.18207303151597476No Hit
TCTACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA3320.15991599593466566No Hit
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2750.13246053880130437No Hit
TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2740.13197886411475418No Hit
TCTACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.129088815995453No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2590.1247537438165012No Hit
TCTACCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2580.12427206912995102No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2580.12427206912995102No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC2570.12379039444340083No Hit
TCTACCGGGACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAG2480.11945532226444903No Hit
TCTACCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2470.11897364757789883No Hit
TCTACCGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG2450.11801029820479846No Hit
TCTACCGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2450.11801029820479846No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG2450.11801029820479846No Hit
TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2390.11512025008549726No Hit
TCTACCGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCAT2290.11030350321999528No Hit
TCTACCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2250.10837680447379448No Hit
TCTACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGT2080.10018833480244112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCA150.00825649450.25054646
ATTAGTT150.00828031950.21393644
AAATCGC150.00831216550.1652141
TAAGTAG204.1084317E-450.1652141
AATCGCC150.00831216550.1652142
ATAAGTA204.1232124E-450.12872740
CCTATTA150.00834410150.11657737
ACGACAT150.00834410150.11657738
TAGGATT150.00836811450.08016634
CGTACTC204.1529007E-450.0559233
TCTTCGA150.008392177550.04380432
AGTGCGA150.008392177550.04380430
AGCTATT150.008392177550.04380430
TCCATCG150.0084002150.03169629
CGTGATG150.00852951249.83875721
CTCATAC204.2429852E-449.83875720
GTCAAAC150.00854577949.81474319
GGTACGT150.0085539249.8027468
ACTGAAA150.0085539249.80274610
GGACTAA150.0085539249.8027468
Produced by FastQC (version 0.11.5)