##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	207609
Sequences flagged as poor quality	0
Sequence length	56
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.84965006334022	39.0	39.0	40.0	38.0	40.0
2	38.729361443868044	39.0	39.0	40.0	37.0	40.0
3	38.660515680919424	39.0	39.0	40.0	36.0	40.0
4	38.6538444865107	39.0	39.0	40.0	36.0	40.0
5	38.66341054578559	39.0	39.0	40.0	36.0	40.0
6	38.82487753421094	39.0	39.0	40.0	38.0	40.0
7	38.865945117986215	39.0	39.0	40.0	38.0	40.0
8	38.863329624438244	39.0	39.0	40.0	38.0	40.0
9	38.80805745415661	39.0	39.0	40.0	38.0	40.0
10	38.15706929853715	39.0	38.0	40.0	35.0	40.0
11	38.05573457798072	39.0	38.0	40.0	35.0	40.0
12	37.940980400657004	39.0	38.0	40.0	35.0	40.0
13	37.69983960232938	39.0	38.0	40.0	34.0	40.0
14	37.91211363669205	39.0	38.0	40.0	35.0	40.0
15	37.87165296302184	39.0	38.0	40.0	35.0	40.0
16	37.671035456073675	39.0	38.0	40.0	34.0	40.0
17	37.576925855815496	39.0	38.0	40.0	33.0	40.0
18	37.47482527250745	39.0	37.0	40.0	33.0	40.0
19	37.37476698987038	39.0	37.0	40.0	33.0	40.0
20	37.32909941283856	39.0	37.0	40.0	33.0	40.0
21	37.19003511408465	39.0	37.0	40.0	33.0	40.0
22	37.031906131237086	39.0	36.0	40.0	33.0	40.0
23	36.89698423478751	39.0	36.0	40.0	32.0	40.0
24	36.813052420656135	39.0	36.0	40.0	32.0	40.0
25	36.571588900288525	38.0	36.0	40.0	31.0	40.0
26	36.14433863657163	38.0	35.0	40.0	31.0	40.0
27	35.91289876643113	38.0	35.0	40.0	30.0	40.0
28	35.655082390455135	38.0	35.0	39.0	29.0	40.0
29	35.5175016497358	38.0	35.0	39.0	29.0	40.0
30	35.22970102452206	38.0	35.0	39.0	29.0	40.0
31	34.55165720175908	38.0	33.0	39.0	27.0	40.0
32	34.26476694170291	38.0	33.0	39.0	26.0	40.0
33	34.0375031910948	38.0	33.0	39.0	25.0	40.0
34	34.28047435323131	38.0	33.0	39.0	26.0	40.0
35	33.97263606105708	38.0	33.0	39.0	25.0	40.0
36	33.89854004402507	38.0	33.0	39.0	23.0	40.0
37	33.661748768116986	38.0	33.0	39.0	23.0	40.0
38	33.441112861195805	37.0	33.0	39.0	22.0	40.0
39	33.2712743667182	37.0	33.0	39.0	20.0	40.0
40	32.98648902504227	37.0	32.0	39.0	18.0	40.0
41	32.554311229281964	37.0	32.0	39.0	10.0	40.0
42	32.33712892986335	36.0	32.0	39.0	8.0	40.0
43	32.11099229802176	36.0	31.0	39.0	4.0	40.0
44	31.86611852087337	36.0	31.0	39.0	4.0	40.0
45	31.460293147214234	36.0	31.0	39.0	4.0	40.0
46	30.74097943730763	36.0	30.0	39.0	4.0	40.0
47	30.472373548352913	35.0	29.0	39.0	4.0	40.0
48	30.20116179934396	35.0	29.0	39.0	4.0	40.0
49	29.85778073204919	35.0	29.0	38.0	4.0	40.0
50	29.44178238901011	35.0	28.0	38.0	4.0	40.0
51	28.6306952010751	35.0	26.0	38.0	4.0	40.0
52	28.254439836423277	34.0	25.0	38.0	4.0	40.0
53	27.90764851234773	34.0	23.0	38.0	4.0	40.0
54	27.447721437895275	34.0	19.0	38.0	4.0	40.0
55	27.03220958628961	33.0	4.0	38.0	4.0	40.0
56	15.86924940633595	19.0	4.0	23.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.2900910373745731
1	2	-0.367535930917974
1	3	-0.5277572676517579
1	4	-0.5629506476288384
1	5	-0.6653780267616511
1	6	-0.3997501315471226
1	7	-0.574384882843411
1	8	-0.5872109390463933
1	9	-0.4398881912941448
1	10	-0.4618222505960716
1	11	-0.5173913586831702
1	12	-0.4521271500375903
1	13	-0.3448632275218415
1	14	-0.5267064120620546
1	15	-0.7805616263047881
1	16	-0.08414913404671154
1	17	-0.27081440278492863
1	18	-0.4379969116951372
1	19	-0.3039850256336507
1	20	-0.16845958153978557
1	21	-0.18881849015192387
1	22	-0.3642204090852559
1	23	0.06603803874386216
1	24	0.018145375841974953
1	25	-0.5631411667728656
1	26	0.148769830377546
1	27	-0.5459142726910358
1	28	0.1620871523763654
1	29	-0.11721147683471855
1	30	0.39828359618734765
1	31	-0.46539611463466457
1	32	0.04916901248825667
1	33	0.1806671830093194
1	34	-0.6686126599552793
1	35	-1.2820526621320525
1	36	0.3313188047273954
1	37	0.7074760801639997
1	38	0.40168637616149994
1	39	0.22129517535295662
1	40	0.16501623187944858
1	41	0.8167309919268959
1	42	0.6159284862451244
1	43	0.8877754192606346
1	44	0.7938042393452314
1	45	0.6878969596108107
1	46	0.6707505672348653
1	47	0.7238604786981924
1	48	0.02725452027574704
1	49	0.8312109990829271
1	50	0.5891879431967553
1	51	1.2509376604097113
1	52	1.190390482564414
1	53	0.3984295714309063
1	54	1.1142144306181372
1	55	0.8274105350395722
1	56	1.494321684207609
2	1	0.0024894318016421835
2	2	-0.12410985344934744
2	3	-0.11733719127423115
2	4	-0.012159594710126953
2	5	0.05276163499503639
2	6	-0.10752427229998318
2	7	-0.020784228178925446
2	8	-0.033419340573949796
2	9	-0.0787043396847622
2	10	-0.19452819713180958
2	11	-0.3518703548642961
2	12	-0.19200712821544386
2	13	-0.0341976519626499
2	14	-0.12487880704296117
2	15	0.027212514448073932
2	16	-0.00220696274283938
2	17	0.036823349533669614
2	18	0.1247690457516697
2	19	0.20558944245145483
2	20	0.07311161322289905
2	21	0.2833582692588763
2	22	0.1820971031897045
2	23	0.24287928260638836
2	24	0.09662328091562244
2	25	-0.3306534417319469
2	26	0.4041708123742751
2	27	0.6253350453667892
2	28	0.6872917350277561
2	29	0.4785332040163439
2	30	0.3324625378130932
2	31	0.33763170860591885
2	32	0.17731958531967962
2	33	-0.5735881361396196
2	34	0.15121821838623362
2	35	0.4072817623087559
2	36	0.304122950935799
2	37	0.6504384369561436
2	38	1.0750360761069473
2	39	0.9727681704429756
2	40	0.4229267610665701
2	41	1.3129666711412966
2	42	0.9593545637137524
2	43	0.8499958229703992
2	44	0.8445952922639428
2	45	0.817411416784843
2	46	0.7196867374476312
2	47	0.9244060324352326
2	48	1.0659615579189534
2	49	1.012280284407531
2	50	0.7232850517619482
2	51	1.0574297498805265
2	52	1.0117761890565014
2	53	0.6363728338422519
2	54	0.15799511801584742
2	55	0.7425496512425198
2	56	0.45037733068878794
3	1	-0.18725415794195044
3	2	-0.19371893289449105
3	3	-0.30539940228726437
3	4	-0.06882205792996388
3	5	-0.29393865924624407
3	6	-0.26775966532225937
3	7	-0.04180146231091442
3	8	-0.043843888703413825
3	9	-0.10199143510300246
3	10	-0.24606245522343784
3	11	-0.5137871266171317
3	12	-0.08414668265007919
3	13	-0.182411187061426
3	14	-0.2376993198634736
3	15	-0.2431531854258253
3	16	-0.22145874920774133
3	17	-0.47061675134905556
3	18	-0.4588375116253829
3	19	-0.16490236082723442
3	20	0.1301103521888507
3	21	0.34094549078052694
3	22	0.22698991529563273
3	23	0.2991214011435872
3	24	-0.09076637860518133
3	25	0.331725709171792
3	26	0.3431367644549823
3	27	0.6500513127060117
3	28	0.8013732818961827
3	29	0.6096807449999488
3	30	0.664618906035038
3	31	0.8422134656466937
3	32	0.8985469917929834
3	33	1.649362683532516
3	34	1.4076284747964891
3	35	1.194839559702615
3	36	1.3855857504313818
3	37	1.4971807656656893
3	38	1.544140741932921
3	39	1.513499570190767
3	40	0.8125862818736351
3	41	1.3992214000189733
3	42	1.1094176154161488
3	43	1.5301135194815387
3	44	1.0666213536342717
3	45	1.5377981338928706
3	46	1.8989217101252294
3	47	2.1754358769077022
3	48	2.043851427612104
3	49	2.822200418917827
3	50	2.6815027903075546
3	51	3.065668505660266
3	52	3.5129111196996305
3	53	3.1533127245526344
3	54	3.2588778418493902
3	55	3.258901059397207
3	56	1.5316719923113205
4	1	-0.12617213350913659
4	2	-0.24232706381314983
4	3	-0.1686729410393042
4	4	-0.25538687353949996
4	5	-0.6600588778254775
4	6	-0.27562737338737975
4	7	-0.1287181790448173
4	8	-0.07339786118073732
4	9	-0.14722360401554369
4	10	-0.7136045832238977
4	11	-0.2587303360698243
4	12	0.05360973310822459
4	13	-0.13039848428913103
4	14	-0.3284497561636428
4	15	-0.006778625302231944
4	16	0.09530948241759063
4	17	0.10163741853622099
4	18	-0.22392205372477036
4	19	0.19721247910067063
4	20	-0.4113024020811693
4	21	-0.6389131765727782
4	22	-0.4108952447764622
4	23	-0.7653365852953442
4	24	-0.18311896636588898
4	25	-0.49432641797037036
4	26	-0.7095625746575394
4	27	-0.447050509741274
4	28	-0.3478270728659254
4	29	-0.43769716247579993
4	30	-0.2595363882172421
4	31	0.6279257078004434
4	32	0.5734532945423894
4	33	0.2410715497242535
4	34	0.7619042165067853
4	35	0.4354197674101101
4	36	0.7001224139509645
4	37	0.8021903499646044
4	38	0.9438775313358363
4	39	0.45436301538860846
4	40	0.9435335539246736
4	41	1.0054354588980416
4	42	0.5290954685331926
4	43	0.7321276527462146
4	44	0.8474413118638928
4	45	-0.08340748508117812
4	46	1.194032287185852
4	47	1.0935831031829863
4	48	0.17059305207924425
4	49	0.7578568718689347
4	50	0.8303598268995493
4	51	1.3375478865431631
4	52	0.875140399017571
4	53	1.515255905395481
4	54	0.876078082172107
4	55	1.1814542021889558
4	56	0.17442082646003243
5	1	0.09231573453116937
5	2	0.05033183389556939
5	3	-0.13379707547605335
5	4	0.040115012403191486
5	5	-0.11167178105128528
5	6	-0.11554743193604367
5	7	-0.1034972141177164
5	8	0.018028136679980378
5	9	-0.032715919502791735
5	10	0.04567031403444588
5	11	0.2356399842589454
5	12	0.015271614547174295
5	13	-0.2228659728890321
5	14	-0.08716168545486624
5	15	-0.1454094872061873
5	16	0.23923224321249847
5	17	0.07098381272639642
5	18	-0.7064137499472594
5	19	0.4711808403009172
5	20	0.1985044999061074
5	21	-0.28033072991399166
5	22	-0.4003677437912714
5	23	0.07067084588182126
5	24	-0.5161145189189469
5	25	-0.04752689086345896
5	26	-0.27258683857692745
5	27	-0.07954502080360015
5	28	-0.0551400267588491
5	29	0.5430493330486001
5	30	0.45793813748224466
5	31	0.96534055889542
5	32	0.7780640021931262
5	33	0.8006054122474779
5	34	0.6871983672696089
5	35	0.4561651369985782
5	36	0.7204174091657407
5	37	0.7053598596195059
5	38	0.5878565889274583
5	39	0.2588723888052584
5	40	0.6338448752104924
5	41	1.0405928084448561
5	42	0.51229087471458
5	43	1.3652977253690821
5	44	0.6388053832482292
5	45	2.1083957013175123
5	46	0.5777512535766647
5	47	1.2843729472408576
5	48	1.0832485719801603
5	49	1.3491702761362312
5	50	1.443797872274768
5	51	0.8672725952072398
5	52	0.9044147333079486
5	53	1.4922868123368787
5	54	0.9905013214897913
5	55	1.2230293865609632
5	56	0.5359025581495018
6	1	-0.15283386808687283
6	2	-0.18032501509929233
6	3	-0.3562725312965327
6	4	-0.12631790017277922
6	5	-0.10027626912982868
6	6	-0.037122934507344496
6	7	-0.18215546563712337
6	8	-0.1950971555126344
6	9	-0.007996424411629732
6	10	-0.6314568481908935
6	11	-0.33734973056105844
6	12	-0.3712992129423114
6	13	-0.08734703880768535
6	14	0.07932966564377608
6	15	-0.5507975189967453
6	16	-0.20753026263135155
6	17	-0.22109192359899055
6	18	-0.2850135629439805
6	19	-0.03571490537463262
6	20	-0.17111915600786887
6	21	-0.09665845314914634
6	22	-0.323170455338726
6	23	-0.3376391120424458
6	24	0.06301124078184728
6	25	0.20755726060919244
6	26	-0.26288604381301894
6	27	-0.5976025243099059
6	28	0.4620408989073539
6	29	-0.3258146220706095
6	30	0.1438895166313756
6	31	0.35109324706746037
6	32	0.5554132308046249
6	33	0.28728142907777254
6	34	0.5235705037819969
6	35	0.5147789752407519
6	36	1.0690616354396951
6	37	0.8716758951501262
6	38	0.22916093675354432
6	39	0.5527012808108722
6	40	0.6726480542661264
6	41	1.0682342296697271
6	42	1.4933567933681573
6	43	1.0379835598711793
6	44	1.3663065520186812
6	45	0.543470502626537
6	46	0.537078041795457
6	47	0.9929400346648869
6	48	0.7274164074172838
6	49	0.4544764939950454
6	50	0.33872540850665445
6	51	-0.13342866706484813
6	52	0.2154829895024335
6	53	0.21318442804515314
6	54	0.2825770578151783
6	55	0.8392051696371681
6	56	1.1381699728292567
7	1	-0.06930543999322936
7	2	-0.026673956013446798
7	3	-0.0968243707614107
7	4	-0.1608775434280716
7	5	-0.03954605731265559
7	6	-0.030601195376910084
7	7	0.08690807951337831
7	8	0.0691447619901524
7	9	0.0495007885203691
7	10	0.21060000799639766
7	11	-0.02366522665916193
7	12	0.11055697434866119
7	13	-0.06496688273188056
7	14	0.20845452629037453
7	15	0.05798174521730459
7	16	-0.3970787576146293
7	17	0.006054118764438954
7	18	0.186307507290131
7	19	0.22610226296427527
7	20	0.09362443373571949
7	21	-0.04997506407445229
7	22	0.33081505190765625
7	23	-0.1468643071372071
7	24	0.07098225527460045
7	25	0.2488337377552341
7	26	0.33237594057940356
7	27	0.10225812228986797
7	28	0.7488301965662174
7	29	0.5554562809394241
7	30	0.8709240762746333
7	31	0.6966060675802836
7	32	-0.04832144032134522
7	33	0.5443605818091015
7	34	0.5255771927452102
7	35	0.550871505898499
7	36	0.9861742329870751
7	37	1.0094127959305013
7	38	0.6083694094402787
7	39	1.3266143242891317
7	40	1.1306190687588824
7	41	1.6052743634489914
7	42	0.754226358585548
7	43	1.3730727460473275
7	44	2.039467087135737
7	45	1.7404883398617699
7	46	2.617122634883529
7	47	2.6372265452557464
7	48	2.137756429713825
7	49	2.4994597715870164
7	50	2.779695308172208
7	51	2.498455390906166
7	52	2.432289009569324
7	53	2.55432155179097
7	54	2.7323540923748197
7	55	2.660498369191238
7	56	1.8349927153041747
8	1	-0.7757085341058243
8	2	-0.7851039803652569
8	3	-0.9342115645731894
8	4	-1.083811220551695
8	5	-0.8332432353673767
8	6	-0.6965229830864033
8	7	-0.8054855603662929
8	8	-0.7459533614878566
8	9	-0.9465742723591646
8	10	-0.8010888617930263
8	11	-0.2113174254845518
8	12	-0.31686030111330865
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113	40	1.0692434073832189
113	41	0.15895771713233842
113	42	0.6007872051463963
113	43	0.7877247606993407
113	44	0.4309200785887306
113	45	0.16352456289799022
113	46	0.37259679035768656
113	47	0.379269687298887
113	48	0.9415008334582282
113	49	0.08163315376040003
113	50	0.3776603061372015
113	51	0.7016299940807684
113	52	0.21470659198690356
113	53	0.1749971724665933
113	54	0.5137134737342031
113	55	0.606285914978784
113	56	0.391850672162132
114	1	0.042818305826166636
114	2	0.18380541968331698
114	3	0.1437274687034673
114	4	-0.044796161042341964
114	5	0.2964628613049598
114	6	0.1748335872317881
114	7	0.09501844740636045
114	8	0.0929463227482401
114	9	0.07352531471880752
114	10	0.33049967354823906
114	11	0.4017807042215509
114	12	0.4276138305359538
114	13	0.8528703524966588
114	14	0.4978079265133388
114	15	0.6339850896989105
114	16	0.3848610417164693
114	17	0.1484732937923141
114	18	0.4378125240125357
114	19	0.1545024859297115
114	20	0.3017069309486544
114	21	0.3598632859812838
114	22	0.19313389248736712
114	23	0.49388262708799147
114	24	0.45975037121662865
114	25	0.5064703040874505
114	26	0.4327661301000276
114	27	0.2882670409074848
114	28	0.8316061162986585
114	29	0.3154897257554765
114	30	1.2525851688052896
114	31	1.2423975948935464
114	32	1.22932627428289
114	33	1.6296727334256005
114	34	1.5018313733472155
114	35	1.8517354969798774
114	36	1.5147138093527417
114	37	1.632545460367517
114	38	1.5335087628405049
114	39	1.7157447590717396
114	40	1.4063437064400404
114	41	1.9160603166262433
114	42	1.9498785324985946
114	43	1.8586357337842259
114	44	2.2141326389752045
114	45	2.663035720017845
114	46	2.7327302157085
114	47	2.5201139477083636
114	48	2.14589466828685
114	49	2.3729547548646117
114	50	3.1213341041588265
114	51	2.730701767717761
114	52	2.280700380806781
114	53	2.474053993262544
114	54	3.043446623032569
114	55	2.284857343550211
114	56	1.540343885872737
115	1	-0.08672505741284908
115	2	-0.009661339000828661
115	3	-0.06288053681758043
115	4	-0.00784539039592147
115	5	-0.07275729052388868
115	6	0.026541661765946856
115	7	0.08544287804817685
115	8	-0.0014291085837143669
115	9	0.08197942099899791
115	10	0.06359146098785118
115	11	-0.04401524700918458
115	12	-0.052487388695702464
115	13	-0.05039626816126486
115	14	0.0374329552688053
115	15	-0.04018675295119323
115	16	0.09270553216965993
115	17	-0.19826008554976227
115	18	0.2602187812014023
115	19	-0.1563153194533058
115	20	0.3864205265318148
115	21	0.20806319396380246
115	22	0.13504785614046
115	23	0.2992081389352421
115	24	0.7492891335814775
115	25	0.6804983694198654
115	26	0.3058395140429724
115	27	0.7589939790257247
115	28	0.8404052881413051
115	29	1.1504908759740147
115	30	0.7393001446506986
115	31	0.7194794904537005
115	32	0.6296598376599292
115	33	0.5131843506328693
115	34	0.6986337658017803
115	35	0.8203057753327698
115	36	1.4044078512206966
115	37	1.3937025802202854
115	38	0.8947348958057617
115	39	0.8006883983632065
115	40	1.2332645714043835
115	41	0.9473175054921299
115	42	1.5584591628183517
115	43	1.0575660305406132
115	44	1.2245708722395285
115	45	1.35400075337418
115	46	2.409633827394721
115	47	1.3104866185158208
115	48	1.8991727911301908
115	49	1.4043844765117228
115	50	1.7586126870895846
115	51	2.4582437506945247
115	52	2.071849449129761
115	53	2.6829336804030994
115	54	3.0057769657976934
115	55	2.632740941433809
115	56	0.8151310954425526
116	1	-0.22689379758158879
116	2	-0.24815697749647114
116	3	-0.2714605453975878
116	4	-0.3183273008778329
116	5	-0.24924806701466906
116	6	-0.2792498440255571
116	7	-0.0723434797381799
116	8	-0.1879584951131008
116	9	-0.2015040624844815
116	10	-0.4613334639370734
116	11	-0.40065760365154546
116	12	-0.6607527969611127
116	13	-0.49060790837290114
116	14	-0.3476092297733828
116	15	-0.314575427339868
116	16	-0.5342928948287664
116	17	-0.3742646615543421
116	18	-0.40496068397806084
116	19	-0.6538700170080034
116	20	-1.0096326695213804
116	21	-0.8376396517390461
116	22	-0.402518281425678
116	23	-0.7999481602210565
116	24	-1.2551438708515263
116	25	-0.6504039614824677
116	26	-0.8299248217213631
116	27	-1.1560925360607897
116	28	-0.8249743772383553
116	29	-0.7052505797674371
116	30	-0.9658049604544061
116	31	-0.8590418880676722
116	32	-0.8488758408757491
116	33	-0.31953408208556056
116	34	-0.3401192729512559
116	35	-0.9092809542539655
116	36	-1.5791757323628843
116	37	-0.8822018956841902
116	38	-0.27700827593740485
116	39	-0.2896018919270844
116	40	-0.2928043546645469
116	41	-0.3294449712703482
116	42	0.0190912234504097
116	43	-0.44882607585149614
116	44	-0.6129237652551112
116	45	-0.6563238569504293
116	46	-0.1938267760658796
116	47	-0.5659612579320559
116	48	-0.6774895855321894
116	49	-0.4984612263339798
116	50	-0.3949408664639691
116	51	0.4155725080232848
116	52	0.6185675958479102
116	53	-0.008672211202792823
116	54	0.20872276874349538
116	55	0.18080328949615776
116	56	0.03540851571997372
117	1	-0.04968802037581099
117	2	0.011502682163190059
117	3	0.1699236959866539
117	4	0.05035566780513534
117	5	0.03306281529621913
117	6	0.1164887517130424
117	7	0.1648079574132595
117	8	0.08322697607236762
117	9	0.13488947390905537
117	10	-0.03693763954125018
117	11	-0.059888262882203946
117	12	-0.5974609336692467
117	13	-0.2832005009654992
117	14	-0.06838715055130251
117	15	-0.24124495400939594
117	16	-0.06073362126949888
117	17	-0.044820932110610556
117	18	-0.36411984313721746
117	19	-0.08753225067024317
117	20	0.0530871931984791
117	21	-0.1305611446605326
117	22	-0.20886748777488862
117	23	-0.03941619968910004
117	24	-0.0919807076883643
117	25	-0.4041577152362166
117	26	0.36933157753503565
117	27	-0.28862506859332626
117	28	0.43299788073390033
117	29	0.1716548971380405
117	30	0.5223689277194836
117	31	0.5342943052685172
117	32	-0.22219201419191847
117	33	0.052866890315407034
117	34	0.0637131308811476
117	35	0.21184016686715523
117	36	-0.11940167258882894
117	37	-0.24650905999135375
117	38	-0.08410108303021246
117	39	-0.06703646936166052
117	40	-0.29583595769614845
117	41	-0.5235819654073417
117	42	-0.6584720541128632
117	43	-0.9583069853324062
117	44	-0.9447412970726425
117	45	-0.5401791408057122
117	46	-0.9607365429756491
117	47	-0.48316417513497356
117	48	-0.995007103049705
117	49	-0.5585784864512391
117	50	-0.6064666779897827
117	51	0.22543951789029393
117	52	-0.4942881170078053
117	53	-0.4705054730360523
117	54	-0.5921066320859047
117	55	-0.8963595876667192
117	56	-0.33301705270559445
118	1	-0.21661512512675074
118	2	-0.22664738500242265
118	3	-0.1634644664170537
118	4	-0.13510366273191465
118	5	-0.1730122457011234
118	6	-0.08448720573959889
118	7	0.0035282469107542624
118	8	-0.0868802147602068
118	9	0.01174338185104773
118	10	0.11598063772935774
118	11	0.18385436946146427
118	12	-0.09119671237911575
118	13	-0.11029702754388637
118	14	-0.4691328259083747
118	15	-0.25903698734546765
118	16	-0.2596534885831474
118	17	-0.1020898961153236
118	18	-0.27833976253848647
118	19	-0.1353432000355923
118	20	0.02512436730819445
118	21	0.05498042948207171
118	22	0.19955425743442134
118	23	0.3268968194736601
118	24	0.46856429327114313
118	25	0.474368479352151
118	26	0.7986706130916943
118	27	0.6620561826060651
118	28	0.7890852782720756
118	29	0.9085850174878445
118	30	0.5084423438447132
118	31	-0.5549948358340941
118	32	0.22825621345837988
118	33	-0.03576430194272717
118	34	0.19692035735262436
118	35	-0.573613680762854
118	36	-0.9740223924945184
118	37	-0.28483458018215657
118	38	-0.15307605355958742
118	39	-3.6153545876279694
118	40	-0.48008904868498803
118	41	-0.4661485141915094
118	42	-0.883079340896316
118	43	-0.42594412654467817
118	44	-0.1695404856024041
118	45	-0.003978247091865228
118	46	-0.07927699312231695
118	47	-0.41934313722067174
118	48	0.14105123508117146
118	49	0.3786148134363607
118	50	0.4809308601849587
118	51	0.9188353563638536
118	52	1.0503838680786899
118	53	0.4339548718388002
118	54	0.5832641495024937
118	55	0.6358936129802615
118	56	0.76460610709373
119	1	-0.3615350378065827
119	2	-0.04395887076029226
119	3	-0.1787454953221328
119	4	-0.22673723365324605
119	5	-0.08645481691177537
119	6	-0.1250174390317298
119	7	-0.22734416570332883
119	8	-0.06226224048283768
119	9	-0.09117342728075073
119	10	0.008881929120960308
119	11	-0.25742807971136017
119	12	0.18638672835100323
119	13	0.13172711700780582
119	14	-0.046497966225572895
119	15	-0.0019948264343909727
119	16	-0.14592686382314213
119	17	-0.10627179559192967
119	18	0.24558122849278163
119	19	-0.028928324046006537
119	20	0.03890622470279226
119	21	0.09593148284731967
119	22	0.12438527187381254
119	23	0.18087225410189234
119	24	-0.10532908144282516
119	25	0.5101118843125647
119	26	0.08367100761051205
119	27	0.3355524083760315
119	28	0.12055998961580627
119	29	0.10289868624984422
119	30	0.45954831159937726
119	31	0.5268546683872586
119	32	1.330462888716788
119	33	1.2548382597994703
119	34	0.6546673918861714
119	35	1.2159762826783975
119	36	1.3278897087127035
119	37	1.5667211390256455
119	38	1.4761814620239022
119	39	1.3814366593145095
119	40	1.931972706660737
119	41	1.6346378933201322
119	42	1.4902669677226044
119	43	1.6482517125968528
119	44	1.9092054705797104
119	45	1.7490266712427385
119	46	1.3044583110516932
119	47	1.6621907519458397
119	48	0.9010259858767817
119	49	1.3082063549528904
119	50	1.8135102242206251
119	51	1.3302658815999067
119	52	1.9028864324510089
119	53	1.5351884006781233
119	54	2.2997366252395857
119	55	1.7708979526872675
119	56	0.7313613213174506
120	1	-0.21562295226819117
120	2	-0.4792380585775504
120	3	-0.3096721612687787
120	4	-0.3448655412458592
120	5	-0.23984611186803306
120	6	-0.28804800388754614
120	7	-0.41481041475830693
120	8	-0.342530087982567
120	9	-0.3069094678898878
120	10	-0.2277796974045856
120	11	-0.28866795442785076
120	12	-0.17553140535674316
120	13	-0.025714291351626173
120	14	-0.5905361992960962
120	15	-0.3975829029005382
120	16	-0.17457466596160742
120	17	-0.2388995091679078
120	18	-0.29969903935471365
120	19	-0.08058077031450495
120	20	-0.2429276666461675
120	21	-0.14094614972638908
120	22	0.3059923568721956
120	23	-0.07757898253272799
120	24	0.09793260988453056
120	25	0.2506886204611831
120	26	0.1806847239945668
120	27	0.4328091315642837
120	28	-0.03472135826193323
120	29	0.9998097997610245
120	30	0.35041125576181287
120	31	0.9920506938759743
120	32	0.910871140147826
120	33	0.9519437787539999
120	34	0.7941532974915262
120	35	0.7232664868041141
120	36	0.8313188047273954
120	37	1.1064122503767635
120	38	1.0825374399912846
120	39	1.5085292179061511
120	40	1.4735268701773165
120	41	1.0614118429907222
120	42	1.6318859330536313
120	43	1.6430945681968083
120	44	1.650187218068634
120	45	1.549599087270387
120	46	1.085644184256143
120	47	1.734498776570529
120	48	1.63895664793532
120	49	2.267381211848882
120	50	1.8445070921329254
120	51	2.617958937005458
120	52	2.6531564400112195
120	53	1.749493401218139
120	54	2.608895281681967
120	55	2.2954956414225514
120	56	1.0209174288884633
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	47.0
5	4.0
6	9.0
7	43.0
8	106.0
9	121.0
10	117.0
11	180.0
12	222.0
13	272.0
14	450.0
15	635.0
16	750.0
17	1007.0
18	1322.0
19	1442.0
20	1508.0
21	1992.0
22	2024.0
23	2052.0
24	2615.0
25	2885.0
26	2909.0
27	3312.0
28	3914.0
29	4527.0
30	5181.0
31	6011.0
32	7230.0
33	9456.0
34	12462.0
35	16823.0
36	23989.0
37	32621.0
38	36505.0
39	22866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.36804281124614	0.33717228058513843	0.10404173229484272	0.1907431758738783
8	99.16404477058208	0.6119095915623931	0.11949100685627835	0.10455463099924354
9	96.18128308503003	2.3134835195005996	0.6680827902451243	0.8371506052242437
10	49.67414707454879	34.48694420762106	5.462672620165793	10.37623609766436
11	34.731153273702006	23.77160913062536	20.333415218030044	21.16382237764259
12	31.830989985983265	16.49254126747877	26.297511186894596	25.378957559643368
13	25.369805740598917	16.340332066528905	30.046866947001334	28.242995245870844
14	25.0846543261612	19.592117875429295	30.122489872789714	25.200737925619794
15	27.37742583414014	19.391259531137862	28.074409105578273	25.156905529143724
16	30.06613393446334	20.180724342393635	25.15835055320338	24.594791169939644
17	31.701901169987813	20.762587363746274	21.185979413223897	26.34953205304202
18	29.57675245292834	21.10698476462966	23.004783029637444	26.31147975280455
19	29.49053268403586	21.163340702956038	23.081850979485473	26.264275633522633
20	28.888792551167374	22.823369604485485	23.025678819670237	25.262159024676905
21	29.72077318420685	19.32045335221498	25.785972669778285	25.172800793799883
22	30.181735859235392	19.41967833764432	24.199336252281935	26.199249550838356
23	29.954048013564023	22.42977149242804	23.439848175407498	24.176332318600437
24	29.444208944295653	21.896857357559877	22.170947417098596	26.487986281045877
25	27.476127614881335	22.07581349187231	22.257927751707925	28.190131141538433
26	27.796789897334556	20.980382705933387	23.23420253530631	27.98862486142575
27	25.503682424616336	19.273926120151152	24.678510835197038	30.543880620035473
28	27.51603104961188	22.01629622486862	24.079359722289187	26.38831300323032
29	28.154459228668742	20.749428304016828	24.702573355590076	26.39353911172435
30	27.358454124940852	20.550829044064393	25.28222262995761	26.80849420103715
31	30.154152355279873	20.594387520953802	24.711236069042478	24.540224054723843
32	31.13118674364115	20.130437933283414	26.68615183941641	22.052223483659024
33	30.018070950222747	20.017684405832956	27.410829041080003	22.553415602864295
34	28.102345003576467	22.289616641212522	29.670191582732425	19.93784677247859
35	26.751241303622624	24.21255179149	28.775037589622848	20.261169315264528
36	27.475370828033483	25.23613536009131	25.48085526220335	21.807638549671854
37	27.7345677220884	24.087993731317294	26.45664644823016	21.720792098364143
38	28.608546710793707	24.623565387369602	26.029147070777448	20.738740831059243
39	28.64335231013516	22.616281321405058	26.388404786801438	22.35196158165834
40	26.814427246081923	22.1345347964053	27.52949155826854	23.521546399244244
41	24.719329783264932	22.098448985129444	27.96236087624345	25.219860355362172
42	24.34800730871569	21.012072951451323	27.96019948722671	26.679720252606277
43	24.935276493004043	20.034228311564902	29.565310139531277	25.46518505589978
44	25.714992170114563	20.010569133282587	29.00451369866334	25.2699249979395
45	24.633864835505896	20.679120111731844	29.39604671011794	25.29096834264432
46	24.658497778295203	21.48310922250034	29.166424122475114	24.69196887672934
47	25.244226168233368	21.308945496192838	29.06352064214618	24.38330769342761
48	27.454674344756597	20.35551628033444	28.736513978849647	23.453295396059314
49	25.606298906817344	21.207840624453894	29.08487213840508	24.100988330323684
50	23.488012900217594	21.89005672987255	30.094517407522538	24.527412962387316
51	23.57259736539274	21.862967404868147	29.124403377502418	25.4400318522367
52	24.671918560035746	21.718942756952604	29.439905971033635	24.169232711978008
53	24.299305983885617	21.65194290515437	29.66542498166612	24.38332612929389
54	24.287519097426255	21.75441689191836	28.015434637834453	25.942629372820935
55	24.82116128530607	22.180374394961415	27.245689403806157	25.752774915926363
56	25.035832802926873	22.80278111137564	27.328646321732414	24.832739763965076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	2.0
5	4.0
6	14.0
7	24.0
8	21.0
9	18.0
10	92.0
11	166.0
12	188.5
13	224.5
14	238.0
15	239.5
16	241.0
17	395.0
18	549.0
19	675.5
20	802.0
21	1015.5
22	1480.5
23	1732.0
24	2053.0
25	2374.0
26	2720.0
27	3066.0
28	3350.0
29	3675.5
30	3717.0
31	3775.0
32	3833.0
33	3592.5
34	3352.0
35	3663.5
36	3975.0
37	3374.0
38	2832.5
39	2892.0
40	3012.5
41	3133.0
42	3435.0
43	3737.0
44	4169.0
45	4601.0
46	4833.0
47	5546.0
48	6027.0
49	6110.5
50	6194.0
51	6823.0
52	7452.0
53	8316.5
54	9898.5
55	10616.0
56	12202.5
57	13789.0
58	14481.5
59	15174.0
60	16825.5
61	18477.0
62	17837.0
63	15462.5
64	13728.0
65	12003.5
66	10279.0
67	9359.0
68	8439.0
69	7225.5
70	6012.0
71	5245.0
72	3878.5
73	3279.0
74	2928.5
75	2578.0
76	2035.5
77	1493.0
78	1172.0
79	676.5
80	502.0
81	395.0
82	288.0
83	208.5
84	129.0
85	92.0
86	55.0
87	36.0
88	11.5
89	6.0
90	3.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.02986383056611226
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.002890048119301186
21	0.0
22	0.0
23	4.8167468655019774E-4
24	0.00626177092515257
25	0.022638710267859293
26	0.06695278143047748
27	0.06598943205737709
28	0.09585326262348935
29	0.15943432124811543
30	0.24228236733474948
31	0.29189486004941984
32	0.2957482575418214
33	0.311643522197978
34	0.3376539552716886
35	0.3708895086436523
36	0.4055700860752665
37	0.41761195323902145
38	0.4494024825513344
39	0.5014233486987558
40	0.5751195757409361
41	0.5914965150836428
42	0.6165435987842531
43	0.6488158027831163
44	0.6492974774696666
45	0.674826235856827
46	0.7037267170498389
47	0.7465957641528065
48	0.7971716062405771
49	0.7730878719130674
50	0.8289621355528903
51	0.7986166303002279
52	0.8260720874335891
53	0.8217370152546373
54	1.6343222114648208
55	0.8848363991927132
56	0.8631610382979543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	207609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.87495725137157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.78846153846155	27.030620059823995
2	12.901043679938152	10.288571304712224
3	6.288654812524159	7.522795254540989
4	3.5043003478933126	5.589353062728494
5	2.2758020873598763	4.5373755473028625
6	1.5316969462698105	3.6645810152739045
7	1.0557595670660997	2.9468857323141098
8	0.8032953227676844	2.5625093324470516
9	0.5254638577502899	1.885756397844024
>10	3.0488983378430614	21.26834578462398
>50	0.17998647081561653	4.9545058258553345
>100	0.09059721685349827	6.06524765304009
>500	0.004831851565519907	1.1319355133929647
>1k	0.0012079628913799767	0.5515175160999763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1145	0.5515175160999763	No Hit
TCTACCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG	640	0.30827179939212657	No Hit
TCTACCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	618	0.2976749562880222	No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	575	0.2769629447663637	No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	517	0.24902581294645224	No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	415	0.19989499491833207	No Hit
TCTACCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	378	0.18207303151597476	No Hit
TCTACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA	332	0.15991599593466566	No Hit
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	275	0.13246053880130437	No Hit
TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	274	0.13197886411475418	No Hit
TCTACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	268	0.129088815995453	No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	259	0.1247537438165012	No Hit
TCTACCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	258	0.12427206912995102	No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	258	0.12427206912995102	No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC	257	0.12379039444340083	No Hit
TCTACCGGGACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAG	248	0.11945532226444903	No Hit
TCTACCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT	247	0.11897364757789883	No Hit
TCTACCGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG	245	0.11801029820479846	No Hit
TCTACCGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT	245	0.11801029820479846	No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG	245	0.11801029820479846	No Hit
TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA	239	0.11512025008549726	No Hit
TCTACCGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCAT	229	0.11030350321999528	No Hit
TCTACCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	225	0.10837680447379448	No Hit
TCTACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGT	208	0.10018833480244112	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.001445024059650593	0.0	0.0	0.0	0.0
8	0.001445024059650593	0.0	0.0	0.0	0.0
9	0.002408373432750989	0.0	0.0	0.0	0.0
10	0.005780096238602372	0.0	0.0	0.0	0.0
11	0.010115168417554152	0.0	0.0	0.0	0.0
12	0.01059684310410435	0.0	0.0	0.0	0.0
13	0.012041867163754943	0.0	0.0	0.0	0.0
14	0.017340288715807117	0.0	0.0	0.0	0.0
15	0.020230336835108303	0.0	0.0	0.0	0.0
16	0.02312038495440949	0.0	0.0	0.0	0.0
17	0.02552875838716048	0.0	0.0	0.0	0.0
18	0.02745545713336127	0.0	0.0	0.0	0.0
19	0.02986383056611226	0.0	0.0	0.0	0.0
20	0.03612560149126483	0.0	0.0	0.0	0.0
21	0.03805230023746562	0.0	0.0	0.0	0.0
22	0.04094234835676681	0.0	0.0	0.0	0.0
23	0.04672244459536918	0.0	0.0	0.0	0.0
24	0.049130818028120166	0.0	0.0	0.0	0.0
25	0.05105751677432096	0.0	0.0	0.0	0.0
26	0.05153919146087116	0.0	0.0	0.0	0.0
27	0.05683761301292333	0.0	0.0	0.0	0.0
28	0.06839780549012808	0.0	0.0	0.0	0.0
29	0.07225120298252966	0.0	0.0	0.0	0.0
30	0.08332972077318421	0.0	0.0	0.0	0.0
31	0.0871831182655858	0.0	0.0	0.0	0.0
32	0.09344488919073836	0.0	0.0	0.0	0.0
33	0.09681661199658974	0.0	0.0	0.0	0.0
34	0.10307838292174232	0.0	0.0	0.0	0.0
35	0.12138202101064982	0.0	0.0	0.0	0.0
36	0.126680442562702	0.0	0.0	0.0	0.0
37	0.1295704906820032	0.0	0.0	0.0	0.0
38	0.13197886411475418	0.0	0.0	0.0	0.0
39	0.13727728566680636	0.0	0.0	0.0	0.0
40	0.14016733378610755	0.0	0.0	0.0	0.0
41	0.1445024059650593	0.0	0.0	0.0	0.0
42	0.1498008275171115	0.0	0.0	0.0	0.0
43	0.15509924906916367	0.0	0.0	0.0	0.0
44	0.15991599593466566	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCA	15	0.008256494	50.250546	46
ATTAGTT	15	0.008280319	50.213936	44
AAATCGC	15	0.008312165	50.16521	41
TAAGTAG	20	4.1084317E-4	50.16521	41
AATCGCC	15	0.008312165	50.16521	42
ATAAGTA	20	4.1232124E-4	50.128727	40
CCTATTA	15	0.008344101	50.116577	37
ACGACAT	15	0.008344101	50.116577	38
TAGGATT	15	0.008368114	50.080166	34
CGTACTC	20	4.1529007E-4	50.05592	33
TCTTCGA	15	0.0083921775	50.043804	32
AGTGCGA	15	0.0083921775	50.043804	30
AGCTATT	15	0.0083921775	50.043804	30
TCCATCG	15	0.00840021	50.031696	29
CGTGATG	15	0.008529512	49.838757	21
CTCATAC	20	4.2429852E-4	49.838757	20
GTCAAAC	15	0.008545779	49.814743	19
GGTACGT	15	0.00855392	49.802746	8
ACTGAAA	15	0.00855392	49.802746	10
GGACTAA	15	0.00855392	49.802746	8
>>END_MODULE