FastQCFastQC Report
Fri 17 Jun 2016
SRR1527022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199932
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11450.5726947162035092No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.3896324750415141No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.1755596902947002No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.17005781965868397No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2610.13054438509093091No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2540.1270431946861933No Hit
GTAATGGGGGTCTCATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGA2500.12504251445491465No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.12054098393453774No Hit
GTAATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.11453894324070182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC150.004522027458.5099958
GGGCATA258.531479E-658.1276747
TAGAACG202.0094158E-457.98195334
AATAACG202.0094158E-457.98195334
TAACGTA150.00468733657.9819536
TATGCGT150.00468733657.9819532
ACGATGA150.00468733657.9819536
CGTACAC150.00468733657.9819539
CGATGAT150.00468733657.9819537
AAATCGG150.00470597757.92386624
TGATATC202.0219016E-457.90936716
GATATCA202.0219016E-457.90936717
CTCATAG407.239578E-1057.90936712
ACTAGCG150.004710646357.90936312
TAAATCG150.00471531957.89486723
ATTAGAA150.00472467457.86590220
GGTACTA150.004729356657.8514259
ATGGGGG100100.056.995314
AATGGGG195250.055.865083
TAATGGG204950.055.716912
Produced by FastQC (version 0.11.5)