FastQCFastQC Report
Fri 17 Jun 2016
SRR1527021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273233
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17610.6445048731302588No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.43845362749009087No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC8390.3070639344442289No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC7630.2792488462228208No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC7630.2792488462228208No Hit
GTAATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT6210.22727854980913725No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6170.22581459779748422No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5760.21080908967804035No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4890.17896813342458634No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4770.17457627738962717No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA4470.16359663730222923No Hit
GTAATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3840.14053939311869357No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.13834346510121398No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3650.13358562106334154No Hit
GTAATGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCCAGAGGCCC3590.13138969304586195No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC3530.12919376502838237No Hit
GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3500.12809580101964257No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3150.11528622091767833No Hit
GTAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2960.1083324488623263No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2890.10577053284193344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC150.004640700858.13432354
CGTGTCA150.004644078758.12364255
CGAATGA150.00465760858.0809647
CATGTTA150.00465760858.0809645
TACGTAG258.599383E-658.05964740
CCCGAAT201.997464E-458.05964342
TAACGTA150.004664383458.0596436
TTGAATC150.004664383458.0596439
ACGTACT550.058.0596441
GTACGTA303.7244354E-758.0596439
ATAACGT150.00467456158.02769535
AGGCATA150.00468135558.00641632
ATTAGCG202.0083626E-457.99578529
CATTAGG150.004684754657.9957829
ATGGGTT150.004701780657.9426734
GGATAGC150.004701780657.9426738
ACGCGAT150.004705191657.9320624
GCTCAAT150.004705191657.9320624
TAGACCT202.0247995E-457.90025715
CGTTTTA202.0247995E-457.90025712
Produced by FastQC (version 0.11.5)