FastQCFastQC Report
Fri 17 Jun 2016
SRR1527019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241499
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13980.578884384614429No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12850.5320933005933771No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12670.5246398535811743No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC10810.44762090112174374No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC6830.2828169060741452No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC6120.2534171984149003No Hit
GTAATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG5970.2472059925713978No Hit
GTAATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5110.21159507906865038No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA4220.17474192439720246No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3950.16356175387889804No Hit
GTAATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.15279566375016046No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.1482407794649253No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3570.14782669907535848No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC3240.13416204621965308No Hit
GTAATGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCC3050.1262945188178833No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2690.1113876247934774No Hit
GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2650.10973130323521008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGCA150.0080104450.63906549
TAACCGA150.0080104450.63906550
ACAGATA150.0080104450.63906549
CGATACA150.00823974550.2788746
CGTGTTA150.00825338350.25784344
ACAGGTA150.00826020950.24733743
CCGATTG204.07673E-450.24733443
CCCGATT204.07673E-450.24733442
ACCCGAT204.080939E-450.23683541
GTCTACG150.00828755550.20534540
CGGTAAG150.00828755550.20534538
CTTCGTC150.00829440150.19486237
CCGGTAA150.00829440150.19486237
TACCATC150.00830810750.17390436
CGCCCAA150.00837689150.06937433
GTTAGAC150.00838379250.05894532
CTAAGGC150.00838379250.05894532
TACGGAC150.00838379250.05894532
CATAAAC150.00839069850.0485231
TTGTTAG204.165842E-450.02768730