##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241499
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.610876235512364	39.0	38.0	40.0	36.0	40.0
2	38.34309872918728	39.0	38.0	40.0	35.0	40.0
3	38.194443869332794	39.0	38.0	40.0	35.0	40.0
4	38.373541919428234	39.0	38.0	40.0	35.0	40.0
5	38.42401831891643	39.0	38.0	40.0	36.0	40.0
6	38.253404776003215	39.0	38.0	40.0	35.0	40.0
7	38.66330709443931	39.0	38.0	40.0	37.0	40.0
8	38.61003565232154	39.0	38.0	40.0	37.0	40.0
9	38.509567327400944	39.0	38.0	40.0	36.0	40.0
10	37.734168671505884	39.0	38.0	40.0	34.0	40.0
11	37.54207263798194	39.0	37.0	40.0	33.0	40.0
12	37.53010157391956	39.0	37.0	40.0	33.0	40.0
13	37.38432871357645	39.0	37.0	40.0	33.0	40.0
14	37.614627803841834	39.0	38.0	40.0	33.0	40.0
15	37.645439525629506	39.0	38.0	40.0	33.0	40.0
16	37.39884223123077	39.0	37.0	40.0	33.0	40.0
17	37.27061809779751	39.0	37.0	40.0	33.0	40.0
18	37.155760479339456	39.0	36.0	40.0	33.0	40.0
19	37.01836860608118	39.0	36.0	40.0	33.0	40.0
20	36.99162729452296	39.0	36.0	40.0	33.0	40.0
21	36.83592478643804	39.0	36.0	40.0	32.0	40.0
22	36.68677717091996	39.0	36.0	40.0	32.0	40.0
23	36.56822595538698	38.0	36.0	40.0	31.0	40.0
24	36.44190659174572	38.0	36.0	40.0	31.0	40.0
25	36.26494933726433	38.0	35.0	40.0	31.0	40.0
26	35.79280659547244	38.0	35.0	40.0	30.0	40.0
27	35.56147230423314	38.0	35.0	40.0	29.0	40.0
28	35.30564101714707	38.0	35.0	39.0	29.0	40.0
29	35.12853883452934	38.0	34.0	39.0	28.0	40.0
30	34.806504374759314	38.0	34.0	39.0	27.0	40.0
31	34.05132526428681	38.0	33.0	39.0	25.0	40.0
32	33.853854467306284	38.0	33.0	39.0	23.0	40.0
33	33.68192414875424	37.0	33.0	39.0	23.0	40.0
34	33.67898003718442	37.0	33.0	39.0	23.0	40.0
35	33.374071942326886	37.0	33.0	39.0	23.0	40.0
36	33.047706201682	37.0	33.0	39.0	16.0	40.0
37	32.82997445123996	37.0	32.0	39.0	16.0	40.0
38	32.55815137950882	36.0	32.0	39.0	12.0	40.0
39	32.41527294108878	36.0	32.0	39.0	10.0	40.0
40	32.07973117901109	36.0	31.0	39.0	4.0	40.0
41	31.660694247181148	36.0	31.0	39.0	4.0	40.0
42	31.480904682835124	36.0	31.0	39.0	4.0	40.0
43	31.310605841017974	36.0	31.0	39.0	4.0	40.0
44	31.045842839928945	36.0	30.0	39.0	4.0	40.0
45	30.669062811854293	35.0	30.0	39.0	4.0	40.0
46	29.93902252183239	35.0	29.0	39.0	4.0	40.0
47	29.666251206009136	35.0	28.0	39.0	4.0	40.0
48	29.3490076563464	35.0	28.0	39.0	4.0	40.0
49	29.107192990447164	35.0	27.0	38.0	4.0	40.0
50	28.609505629422895	34.0	26.0	38.0	4.0	40.0
51	27.757647029594324	34.0	23.0	38.0	4.0	40.0
52	27.39229562027172	33.0	19.0	38.0	4.0	40.0
53	27.11329653538938	33.0	16.0	38.0	4.0	39.0
54	26.659609356560484	33.0	4.0	38.0	4.0	39.0
55	26.27357877258291	33.0	4.0	37.0	4.0	39.0
56	15.31980670727415	19.0	4.0	22.0	4.0	25.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.5698667932263746
1	2	-0.5942805587667621
1	3	-0.8679262989991869
1	4	-1.0404641576165332
1	5	-1.169975278937379
1	6	-0.7221785583431668
1	7	-0.8647520370120674
1	8	-0.6638377617247997
1	9	-0.6266260072411782
1	10	-0.6672714148563017
1	11	-1.3098890655323316
1	12	-1.210727567159573
1	13	-0.63031033282914
1	14	-0.972402482497607
1	15	-1.192994422375918
1	16	-0.2912770450166988
1	17	-0.6716851669325337
1	18	-0.28790140728498415
1	19	-0.2027151341967368
1	20	-0.43333507525834136
1	21	-0.469952236336006
1	22	-0.5584263591458694
1	23	-0.9659424311052405
1	24	-0.30843851630108077
1	25	-0.6894975936617982
1	26	-0.04674063530164574
1	27	-0.6091044003253003
1	28	-0.03036514891902442
1	29	0.03330041419453522
1	30	-0.01012103757091154
1	31	0.6669263992259147
1	32	0.3925592876764057
1	33	0.015507120041469591
1	34	-0.5749605056631637
1	35	-0.43996384324471904
1	36	0.5421914510414751
1	37	0.8741747883753916
1	38	1.0438357485645255
1	39	0.7702606351607955
1	40	1.2073468440488284
1	41	1.7341140141820048
1	42	1.216336478297066
1	43	1.3691743399049656
1	44	1.6928992888539227
1	45	1.8309537502740447
1	46	1.570459264622663
1	47	1.7823714700062432
1	48	1.2170604870603619
1	49	1.6676720564238146
1	50	1.8360448403764948
1	51	2.1135555514631577
1	52	2.8075649045767506
1	53	1.9799987508757617
1	54	2.7727239593328505
1	55	2.322059106511041
1	56	2.264874337455071
2	1	-0.35879373386564595
2	2	-0.5118033898169898
2	3	-0.4298555227434804
2	4	-0.4186605046484928
2	5	-0.33801180861774327
2	6	-0.05088860400527295
2	7	-0.028336511897911976
2	8	-0.08684004482982033
2	9	-0.11406892961561255
2	10	-0.12606136919420408
2	11	-0.6618297048017396
2	12	-0.6156933205842279
2	13	-0.6063948077149846
2	14	-0.1795371856939525
2	15	-0.17838255022979865
2	16	-0.39116288976556035
2	17	-0.09885411670422428
2	18	-0.43904295979640295
2	19	-0.05779504287253445
2	20	-7.437510574277439E-4
2	21	-0.060477350491254356
2	22	-0.13530992078970883
2	23	-0.09864791055729683
2	24	-0.029386004885559203
2	25	-0.7775683699175033
2	26	-0.028133329365573445
2	27	-0.008476546444022404
2	28	0.07203211135494314
2	29	-0.35608314744930425
2	30	0.3923903779542002
2	31	0.7496318786779668
2	32	1.048381205484624
2	33	0.5093427364798231
2	34	0.6071171199076133
2	35	0.4438261110931805
2	36	0.5012097158816573
2	37	0.7498026422566682
2	38	0.8494864334960326
2	39	0.33744100045759495
2	40	0.7006687618570453
2	41	0.772013557561003
2	42	0.15943008560300598
2	43	0.6777359837405861
2	44	0.5796002020959321
2	45	0.14379621602746795
2	46	0.23963734681444393
2	47	0.19384407274597137
2	48	-0.10765412481178416
2	49	0.39067433952883945
2	50	0.39574803672352843
2	51	1.0740007569426098
2	52	1.433877689964877
2	53	0.4913001207387744
2	54	1.2114225894698372
2	55	1.2213741750041898
2	56	0.7657305018386342
3	1	0.08817936030475693
3	2	-0.052068711192177375
3	3	0.2912767642520322
3	4	0.05604054168932748
3	5	-0.14399088186139153
3	6	0.03448135115168327
3	7	0.06220907853557378
3	8	0.10787221227032973
3	9	-0.006071919152233818
3	10	-0.09956660088329272
3	11	-0.7181451009153434
3	12	-0.12718435197436406
3	13	0.42221132083508905
3	14	-0.41460818718871906
3	15	-0.03069944111171452
3	16	0.006632326975847036
3	17	-0.095789841666857
3	18	0.2516894766759421
3	19	-0.003030468881505044
3	20	0.314110509032254
3	21	-0.08278676753161562
3	22	0.40275061732688755
3	23	0.5073872164980102
3	24	0.3921573433954606
3	25	0.21856391707800782
3	26	0.4018741444759826
3	27	-0.03413050758004488
3	28	0.10764496636242171
3	29	0.20972402743858964
3	30	0.8503126500020386
3	31	0.4611602792758731
3	32	0.554547124767133
3	33	0.514350210321382
3	34	0.6876477626579884
3	35	1.0326900871768885
3	36	0.9903352764197706
3	37	1.961854211763395
3	38	1.9585110971731652
3	39	1.2386554997102124
3	40	1.342428851543147
3	41	1.898620433495232
3	42	1.6270496670678796
3	43	1.8693279119917037
3	44	2.3579073769838246
3	45	2.038583211543159
3	46	2.0646747160222176
3	47	2.01299579172953
3	48	2.1135529005992773
3	49	2.511806626524354
3	50	2.381061938068818
3	51	2.588257109707932
3	52	2.9263477945986587
3	53	2.988721645402453
3	54	2.7669906014279846
3	55	2.8111350120744447
3	56	0.9319300534081396
4	1	-0.3363799407621997
4	2	-0.538383849587099
4	3	-0.34580379860555155
4	4	-0.5784306195910247
4	5	-0.3291037626407345
4	6	-0.3142506729707861
4	7	-0.07029053488641779
4	8	-0.15393774597924903
4	9	-0.15142525145468966
4	10	-0.34531424275742495
4	11	-0.057232003652309515
4	12	-0.06181401023940225
4	13	-0.025504003117283958
4	14	-0.31502569144107895
4	15	0.09201974862077122
4	16	0.22234285736087855
4	17	-0.10862423164675761
4	18	-0.4126061781872039
4	19	0.1797049571274627
4	20	-0.2847954751953594
4	21	0.0843502357156396
4	22	0.04443145852063424
4	23	0.20666818139672927
4	24	0.1118496272983478
4	25	0.4823454231859401
4	26	0.4850850614390225
4	27	0.3508625340157465
4	28	0.30162981250437326
4	29	0.4852961628955228
4	30	-0.06680502434464586
4	31	0.4583962464940612
4	32	0.9449329296225528
4	33	0.45531974797408026
4	34	0.742174591171981
4	35	0.9966996743115715
4	36	1.0650028193299335
4	37	0.9260957457049486
4	38	1.2266416059098262
4	39	0.5384467475840324
4	40	1.2036860032363563
4	41	1.310519304687439
4	42	1.669631235028291
4	43	1.5846325354647206
4	44	1.736927788302829
4	45	1.6476755263722929
4	46	1.3859017146305348
4	47	1.148298095734475
4	48	1.0930642659928154
4	49	1.4480019257357348
4	50	1.7078170022407697
4	51	0.9057536305058278
4	52	1.0116075750223494
4	53	1.4749208103939466
4	54	1.772054773377885
4	55	1.8337304968432697
4	56	1.173058088045531
5	1	-0.23739733746924685
5	2	-0.185789876303474
5	3	-0.08920236959032479
5	4	-0.09501185599984296
5	5	-0.18305685366279079
5	6	-0.17127148688815197
5	7	0.12414096557957066
5	8	0.19232662183684823
5	9	0.21701215146546815
5	10	0.01155124341840974
5	11	-0.12872159669363015
5	12	0.07743731004640608
5	13	0.18290699408682087
5	14	-0.0037488974056643087
5	15	-0.18558975743700756
5	16	-0.7841809077835791
5	17	-0.42362889147899807
5	18	-1.2327366534900932
5	19	-0.4306554032328975
5	20	-0.39162212943580954
5	21	-0.583675548689456
5	22	-0.5786657766455647
5	23	-0.06880556821495531
5	24	-0.662381500381052
5	25	-0.0222755771247094
5	26	0.3937135174812738
5	27	0.31055498508750645
5	28	0.6862213005441333
5	29	0.5490970327308773
5	30	0.1504984860623111
5	31	-0.10408433753824653
5	32	-0.038330506227090666
5	33	-0.052368975231885884
5	34	0.004639642430134927
5	35	0.23706935433642684
5	36	0.3054889951609354
5	37	0.6091494891035154
5	38	0.6766260731356724
5	39	0.7864274869440848
5	40	0.9881687618570467
5	41	1.1756171611646096
5	42	0.8072904459633676
5	43	0.842826073830679
5	44	0.9089920939878233
5	45	1.334224143955396
5	46	0.4292769864540844
5	47	0.45724497364687267
5	48	0.12894497428731455
5	49	0.25745361880811757
5	50	0.7094867754622669
5	51	1.3281674236092762
5	52	1.431963275550462
5	53	1.173507327945984
5	54	1.0882243912716412
5	55	1.5587615623915774
5	56	0.30232960093773364
6	1	-0.09361516243707513
6	2	-0.2498986279122235
6	3	-0.3048555227434804
6	4	-0.10229145702944464
6	5	-0.15884514195107613
6	6	-0.1589243182909854
6	7	-0.061669845231243414
6	8	-0.10529242578220277
6	9	-0.07091416771084624
6	10	-0.6784423215751545
6	11	-0.3737344667065017
6	12	-0.4058718920127973
6	13	0.06562900180882991
6	14	-0.31733480474157005
6	15	-0.4307635026107519
6	16	-0.36140098500365525
6	17	-0.06552078337089284
6	18	-0.17445962646306867
6	19	-0.4319021857296832
6	20	-0.3748508939145765
6	21	-0.5060130647769725
6	22	-0.3656670636468533
6	23	-0.6135288629382458
6	24	-0.37968362393317534
6	25	-0.45346122706035885
6	26	0.3251404801582396
6	27	-0.6569884512059261
6	28	0.056555920878757604
6	29	-0.18019029030644873
6	30	-0.22397866966484514
6	31	0.31600092629701493
6	32	0.2090954911989087
6	33	0.30815226028934717
6	34	0.4359861675266643
6	35	0.6560284920455643
6	36	0.7985311444530865
6	37	0.3337312136852404
6	38	0.1307364334960326
6	39	0.5082743337909292
6	40	-0.04754552385723798
6	41	0.7535611766086205
6	42	0.7252038951268176
6	43	0.9670216980263007
6	44	0.8296002020959321
6	45	0.34379621602746724
6	46	-0.1895293198522232
6	47	-0.39157259392069577
6	48	0.06258397042631003
6	49	-0.24950423189973492
6	50	-0.03818053470504523
6	51	0.0826317093235609
6	52	0.24221102329821065
6	53	-0.1319141649755089
6	54	0.12719639899364665
6	55	-0.1262448726148584
6	56	0.09608764469577658
7	1	-0.1582381783100857
7	2	-0.10374783426143352
7	3	-0.33124441163236895
7	4	-0.08143828242626938
7	5	-0.11578958639552184
7	6	-0.16838860400527267
7	7	-0.10000317856457741
7	8	-0.049895600385376326
7	9	0.02037551482883515
7	10	-0.22550581363864808
7	11	-0.4368297048017382
7	12	-0.6376377650286713
7	13	-0.5944503632705391
7	14	-0.9053705190272865
7	15	-0.7828269946742452
7	16	-0.47727400087666894
7	17	-0.7377430055931171
7	18	-0.6679318486852921
7	19	-0.8036283762058716
7	20	-0.8487993066129889
7	21	-0.5210329060468126
7	22	-0.38058769856748853
7	23	-0.6578145772239594
7	24	-0.13133044933000093
7	25	-0.6572905921397236
7	26	-0.5197999960322406
7	27	-0.5143098797773575
7	28	-0.15852344420061115
7	29	-0.042194258560414255
7	30	0.2623903779542047
7	31	0.42990965645574164
7	32	0.26227009437351256
7	33	0.26823162536871337
7	34	0.5821171199076147
7	35	0.5716038888709605
7	36	0.18620971588165247
7	37	0.15119153114555672
7	38	0.6925419890515911
7	39	0.899941000457595
7	40	0.9437243174126024
7	41	1.1609024464498887
7	42	1.2224856411585634
7	43	0.8282915392961385
7	44	1.2837668687625978
7	45	1.1485184382496882
7	46	1.478526235703331
7	47	1.3230107394126378
7	48	1.3765125418548827
7	49	1.4315076728621712
7	50	1.066303592279084
7	51	1.457056312498164
7	52	1.6219332455204345
7	53	1.8774112318498872
7	54	2.1064225894698367
7	55	2.278040841670858
7	56	1.245452724060856
8	1	-0.5787478623060096
8	2	-0.5632177629056727
8	3	-0.5313463484315548
8	4	-0.8369326759023181
8	5	-0.5126295456208041
8	6	-0.32049869574839107
8	7	-0.3380459920202341
8	8	-0.2877957023221782
8	9	-0.3013777981171373
8	10	-0.04754454962233723
8	11	0.2601121912227242
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113	31	-0.03610160826519859
113	32	0.3807544773740261
113	33	0.2891430436994895
113	34	0.20485137489993832
113	35	0.2595711187736711
113	36	0.35800695090469503
113	37	0.3729017205976817
113	38	0.5277698436342817
113	39	0.6194494489983029
113	40	0.21858350840082608
113	41	0.737144125917375
113	42	0.977652051808839
113	43	0.6605700851230729
113	44	0.20143199933095346
113	45	0.3049137275482039
113	46	0.1644453345256558
113	47	0.5094930742820694
113	48	0.23568304876732427
113	49	0.8629669662569484
113	50	0.32789089386638537
113	51	-0.2340445579575423
113	52	0.09445756707701136
113	53	0.08150749401066548
113	54	-0.13216420008469498
113	55	-0.33707436570241356
113	56	0.44977428064047764
114	1	-0.4968074324957783
114	2	-0.265513435479086
114	3	-0.5756888560768161
114	4	-0.3418340206302304
114	5	-0.27956432003326626
114	6	-0.04637947158517619
114	7	-0.1250260096148068
114	8	-0.07936287588005086
114	9	-0.20653468304026745
114	10	-0.40243123220790267
114	11	0.05997394816630219
114	12	0.13173818626508904
114	13	0.5660366991343295
114	14	0.03672993759371934
114	15	0.43714256392544826
114	16	0.20187363991480822
114	17	-0.19223311213801964
114	18	0.36506662924469424
114	19	0.4000245918306575
114	20	0.35205305259553654
114	21	0.38392904220280855
114	22	0.601390992452302
114	23	0.48611236341530883
114	24	0.18471216863042628
114	25	0.36073071684048585
114	26	0.715816442323927
114	27	0.6694344124600846
114	28	1.065525262039877
114	29	0.8780492726420235
114	30	1.0538059030683584
114	31	1.0824515133811659
114	32	0.6573994703248047
114	33	1.13879479127435
114	34	1.4615691747021344
114	35	1.4732781658877059
114	36	1.6928763825483202
114	37	1.2988323226219691
114	38	1.1305937394321077
114	39	1.3501693109598776
114	40	0.718762369162981
114	41	1.6701870735427349
114	42	1.3030944691646482
114	43	1.6111834723250595
114	44	0.980570521730634
114	45	1.2008167639726715
114	46	1.9768519586865878
114	47	1.8462984106455131
114	48	1.664549071535248
114	49	1.6265761660128568
114	50	1.9912959819290066
114	51	1.7573911679015133
114	52	1.6751448132525475
114	53	1.6147019472227946
114	54	1.4576554661821675
114	55	1.5823330791137806
114	56	1.0913583557199136
115	1	-0.014075424006492199
115	2	0.10820834727221751
115	3	0.12853649603586348
115	4	0.1436634390134799
115	5	0.007528097485540286
115	6	-0.06773132700996598
115	7	0.1818747557077245
115	8	0.1383360115082084
115	9	0.295555483529931
115	10	0.38720154160392184
115	11	0.2749073844001373
115	12	0.05713531791342774
115	13	0.45387514533666007
115	14	0.060075490362386574
115	15	0.19532636995799635
115	16	-0.15583425126790473
115	17	0.10231959221596298
115	18	0.15109788527401946
115	19	0.17853124820727828
115	20	0.09943230528060099
115	21	0.05348978565897511
115	22	0.3616971214638127
115	23	0.19683331009998284
115	24	0.5561186899501251
115	25	0.01357012773507904
115	26	0.2698713654701095
115	27	1.0841877728048033
115	28	0.8233231911671481
115	29	0.31962107790280925
115	30	0.9132823967335497
115	31	0.9360755406497958
115	32	0.6945079660480076
115	33	0.43868545948452464
115	34	0.7332908288278048
115	35	1.0407744679006967
115	36	1.6318435186985596
115	37	1.4594857408482227
115	38	0.9313526306791289
115	39	0.8304574323824809
115	40	1.2276053815753585
115	41	1.02976003643424
115	42	1.1065544987485438
115	43	1.4898251856185212
115	44	1.5804804837860758
115	45	1.7677985634453073
115	46	1.958651431321485
115	47	1.741790082135644
115	48	2.015761368145963
115	49	2.020427860655598
115	50	1.3345978019817437
115	51	1.7741768132806364
115	52	1.4449692392606508
115	53	1.9193517639312638
115	54	2.237126814821952
115	55	1.3450830951919848
115	56	0.7689582013691503
116	1	-0.1010426421625894
116	2	0.14058016185695976
116	3	-0.19868740047273548
116	4	-0.10028350319289103
116	5	-0.06959841706024861
116	6	-0.1093493026952288
116	7	0.08625591896089446
116	8	0.09261774265198142
116	9	-0.0329190023958148
116	10	-0.0734048917560628
116	11	0.08485879592606693
116	12	-0.1732806568287728
116	13	0.16349238296915303
116	14	0.007817253898480203
116	15	0.4160861542825742
116	16	0.4492665134367684
116	17	-0.06661248642765827
116	18	0.16546941283824168
116	19	0.038875262804317856
116	20	-0.2403179868652927
116	21	0.22369295518559795
116	22	-0.08962214786394185
116	23	0.2518797459201423
116	24	-0.15960798450709746
116	25	-0.13166225638038753
116	26	0.628380498873149
116	27	0.6945984171658779
116	28	0.8252708304233565
116	29	0.4016679442537523
116	30	0.7330745118697806
116	31	0.22057438231697546
116	32	1.1078790220785137
116	33	1.0203325472513
116	34	0.6953631169964041
116	35	1.088440376013125
116	36	-0.17466364656375788
116	37	0.9391585374531743
116	38	0.6854580492165567
116	39	0.8254140717821983
116	40	1.3811818622937295
116	41	1.2743425240821082
116	42	1.3908893286888855
116	43	0.9754434073213716
116	44	0.8555281496941838
116	45	0.8997459976868569
116	46	0.8172938242525802
116	47	1.053432864594587
116	48	0.6310103584487692
116	49	0.5982616757733794
116	50	0.10393580964929328
116	51	1.2102634934782728
116	52	1.474470848625721
116	53	1.1628066709571172
116	54	1.7456656753504767
116	55	1.410093243417581
116	56	0.8298680564237859
117	1	-0.11593659100849862
117	2	-0.4909700564836541
117	3	-0.3807483798863345
117	4	-0.46836288560087525
117	5	-0.25705942766536083
117	6	-0.3598171754338395
117	7	-0.07952698808838932
117	8	-0.10082814006791807
117	9	-0.20484273913942275
117	10	-0.031120893003723893
117	11	-0.15052018099221698
117	12	-0.3299790348699432
117	13	-0.2066924267626007
117	14	-0.34858480474157005
117	15	-0.6807635026107519
117	16	-0.32568669928937055
117	17	-0.8021279262280387
117	18	-0.8932096264630687
117	19	-0.8649379000153914
117	20	-0.5936008939145765
117	21	-0.6711916362055419
117	22	-0.6692384922182768
117	23	-0.7787074343668152
117	24	-0.5761121953617447
117	25	-0.14096122706035885
117	26	-0.3311095198417604
117	27	-0.21055987977735668
117	28	0.2306630637358964
117	29	0.40016685255069717
117	30	-0.5900500982362757
117	31	0.423143783439869
117	32	0.204631205484624
117	33	-0.5222048825677916
117	34	-0.1399066896161969
117	35	0.24084992061699495
117	36	0.6065668587388018
117	37	0.231052642256671
117	38	0.4923435763531785
117	39	0.47702433379092923
117	40	0.827454476142762
117	41	0.6240968908943358
117	42	0.600203895126814
117	43	0.6009502694548701
117	44	0.3653144878102168
117	45	0.4777247874560402
117	46	0.38636353729063444
117	47	1.0414631203650195
117	48	0.7099053989977406
117	49	0.603174339528838
117	50	0.7118194652949548
117	51	1.301381709323561
117	52	1.4207824518696377
117	53	1.2029072635959182
117	54	1.278982113279362
117	55	0.5255408416708569
117	56	0.5246590732672054
118	1	0.14496732808125756
118	2	0.16500339484377946
118	3	0.005432087875988145
118	4	0.11179672249014772
118	5	0.015380521765735011
118	6	0.25143440484428936
118	7	0.23368413706964475
118	8	0.11120567788404401
118	9	0.2347511293814435
118	10	0.40483833582054274
118	11	0.17762457248439745
118	12	0.30521375906179316
118	13	0.6539665492171736
118	14	0.19763833053023916
118	15	0.47884458841326705
118	16	0.08486955861202006
118	17	0.06501019008043585
118	18	0.18367090451038592
118	19	0.21289079783542775
118	20	0.27879164717266036
118	21	0.3755845964114002
118	22	0.3037387517766632
118	23	0.43545975905922774
118	24	0.1988957060288996
118	25	0.7999758778701036
118	26	-0.007705600752004216
118	27	-0.0808585523437273
118	28	0.4336102824463879
118	29	-0.06471146603337274
118	30	-0.1272261412198361
118	31	-0.6120569118825046
118	32	0.022938727608519116
118	33	-0.30230918092430414
118	34	-0.13587698038737273
118	35	-0.25543642577997616
118	36	-0.37644515137498047
118	37	-0.1359274462389024
118	38	-0.43204011517653385
118	39	-2.7251327458550882
118	40	-0.6578489372579988
118	41	-0.2716442595481432
118	42	-0.5456952831285591
118	43	-0.7074410074098587
118	44	0.04807365342336567
118	45	-0.4830843149459838
118	46	-0.1292417092327547
118	47	0.06224377776072032
118	48	0.015096612651344543
118	49	0.17728938377662473
118	50	0.31145600132529694
118	51	1.1290523793614895
118	52	0.5027198728557316
118	53	0.4663001207387758
118	54	0.20972642427809873
118	55	0.21414703636112264
118	56	0.43331899741385627
119	1	-0.04504914512105529
119	2	-0.09757178808538924
119	3	0.039029758641802914
119	4	-0.03370379469178175
119	5	-0.012470683076614364
119	6	0.014641699025034427
119	7	0.1358409772795781
119	8	0.103582033092259
119	9	-0.11082217636885616
119	10	0.17166590353306788
119	11	0.34325687528484394
119	12	0.4338196664287608
119	13	0.6689839801638087
119	14	0.27939138573461975
119	15	0.42854385669660644
119	16	0.47193234832968045
119	17	0.29486882701871764
119	18	0.27480985405641434
119	19	0.29334132076382957
119	20	0.675067937254255
119	21	0.6469901819762782
119	22	0.7706965727167869
119	23	0.6213196219102386
119	24	0.39588455788500454
119	25	0.7238926690435363
119	26	0.880200003967758
119	27	0.9824866570191801
119	28	0.6983308126536443
119	29	1.280172263806108
119	30	1.1354639710277965
119	31	1.2355085020545928
119	32	0.8703509024543195
119	33	1.239429316566408
119	34	1.1985673363578329
119	35	1.093393210660281
119	36	0.584651274323214
119	37	1.569661949615977
119	38	1.0088479053575057
119	39	1.0575167580333513
119	40	1.087735861424143
119	41	1.0590265445739888
119	42	0.9599170985900187
119	43	0.6878009188055216
119	44	0.9305201155158471
119	45	0.7872485969798468
119	46	1.8459144030914985
119	47	1.8312358476377462
119	48	1.6835255288678717
119	49	1.9348842962387955
119	50	1.8690164349919272
119	51	2.6615007569426083
119	52	2.6191049626921483
119	53	2.74119189563055
119	54	3.05914986219711
119	55	2.3488633524933675
119	56	1.4478192464273771
120	1	-0.46328682142324595
120	2	-0.6839424067140669
120	3	-0.5280698084577651
120	4	-0.35963592707553005
120	5	-0.42065389771144623
120	6	-0.4404623367241669
120	7	-0.38252237979345693
120	8	-0.41520025988358356
120	9	-0.3556203889090028
120	10	-0.3115068377041865
120	11	-0.5517298584115693
120	12	-0.4656357168975944
120	13	-0.4844862055644441
120	14	-0.7221447125756697
120	15	-0.6957404611360971
120	16	-0.5572535195658617
120	17	-0.5090691704676686
120	18	-0.7679216080298872
120	19	-0.47582499678959067
120	20	-0.28513315197908895
120	21	-0.120500391965912
120	22	-0.5200449438311807
120	23	-0.3427915357493063
120	24	-0.23293823222810772
120	25	-0.6514738998714193
120	26	-0.035314588966187443
120	27	-0.42066932678196167
120	28	-0.036762051471477264
120	29	0.05324864978572208
120	30	0.3150478280310054
120	31	0.6551426313661395
120	32	0.6249941087104318
120	33	0.9527375137455749
120	34	0.5504734947156038
120	35	0.5038107501100768
120	36	1.2059332181858053
120	37	1.5065422735930767
120	38	1.1130232998554774
120	39	1.2369609697356267
120	40	1.1079844300598083
120	41	0.13345748997267748
120	42	0.6781128813019279
120	43	0.46702169802630067
120	44	0.20143199933095346
120	45	-0.09139963650709504
120	46	0.049237961253766116
120	47	-0.0527189072847527
120	48	-0.086897596393964
120	49	-0.321364830978073
120	50	-0.4416943595898353
120	51	0.6092734143926855
120	52	0.7949183965701003
120	53	0.6391111806466085
120	54	1.171983265353095
120	55	0.6076260951270775
120	56	0.6663641423916289
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	170.0
5	19.0
6	75.0
7	86.0
8	205.0
9	227.0
10	226.0
11	363.0
12	421.0
13	531.0
14	747.0
15	910.0
16	1119.0
17	1400.0
18	1675.0
19	2040.0
20	2145.0
21	2625.0
22	2627.0
23	2910.0
24	3901.0
25	4604.0
26	3901.0
27	4262.0
28	4825.0
29	5397.0
30	6160.0
31	7332.0
32	8821.0
33	10962.0
34	14447.0
35	19771.0
36	26980.0
37	35715.0
38	39012.0
39	24888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.48074319148319	0.366461144766645	0.11511434829957888	0.03768131545058157
8	99.08833348520871	0.6780545591609851	0.12219065055130765	0.11142130507898901
9	95.91882368042933	2.195868305872902	0.9619087449637473	0.923399268734032
10	54.58324879192047	30.927250216357006	6.16648516142924	8.323015830293294
11	37.93307632743821	25.581886467438785	18.277922475869467	18.207114729253536
12	38.36289177180858	15.968182062865685	23.007548685501803	22.661377479823933
13	26.37526449384884	16.918082476531993	27.605911411641454	29.100741617977715
14	25.00175984165566	20.50774537368685	30.12062161748082	24.369873167176674
15	29.4055047846989	20.127619576064497	27.345454846603918	23.121420792632684
16	32.19185172609411	20.919341280916274	24.61211019507327	22.276696797916347
17	32.58232953345562	21.635700354866895	21.888703472892228	23.893266638785253
18	32.461003979312544	21.88580491016526	22.226178990389194	23.427012120133003
19	29.30860997354026	24.356208514320972	22.46675969672752	23.868421815411246
20	29.98587974177733	25.56760539468399	21.509583969987204	22.936930893551473
21	28.60384515049752	22.737154191114666	26.119362813096537	22.539637845291285
22	27.85891452966679	22.222452266883092	26.214187222307338	23.70444598114278
23	29.49950103313057	23.063449538093327	25.446068099660867	21.990981329115233
24	29.92554125461744	23.941509715251204	23.104159419569648	23.028789610561713
25	28.619178581487432	21.784655146705546	21.700989081992745	27.89517718981428
26	27.07619375183885	20.15174934422901	25.58522122170239	27.18683568222974
27	25.174144196781906	19.190048192671235	25.577752638579828	30.05805497196703
28	25.909283102713548	23.606125173068474	24.072477345647794	26.412114378570184
29	29.34017479747303	22.926094765616252	23.922448657908816	23.811281779001902
30	28.56668382682631	21.221997110450538	26.441868575319262	23.76945048740389
31	29.973706182162573	21.39020773362244	25.83149525838972	22.80459082582526
32	32.001694661815286	20.070527837312465	26.93764641379654	20.99013108707571
33	30.86820327040681	21.216099441637862	27.70248936453071	20.21320792342462
34	26.628020528163894	22.65785700928754	32.678011178291676	18.036111284256894
35	24.886614480920883	27.6984922116308	29.48106638509089	17.933826922357422
36	25.55884102090637	28.28078668180476	26.51495302616312	19.64541927112575
37	27.131228344557073	27.937341158755974	25.850515571121363	19.08091492556559
38	27.30726498749568	26.274878597852002	26.543274092136638	19.87458232251568
39	28.711605871626855	23.526473110580085	26.212917246030493	21.54900377176257
40	26.413003170793697	22.820964737940777	26.877580696907955	23.88845139435757
41	23.203257182387212	24.33843691917887	27.525191488652368	24.93311440978155
42	21.943605026780318	22.665110559989994	28.723089427505577	26.66819498572411
43	22.394706228187584	21.50699039732476	30.15690346038219	25.941399914105467
44	24.23245476872527	21.0167498530166	29.34163945910109	25.409155919157044
45	24.498546420691294	21.89378235100291	28.672842467038993	24.934828761266804
46	23.576906707169503	22.541664232602002	28.597060762599412	25.284368297629083
47	23.048119189650638	22.12973437806563	30.65651990599079	24.16562652629294
48	25.11255899427808	21.793008394937978	30.996115774965542	22.0983168358184
49	25.839530038537173	21.464746627475375	28.7772067020446	23.91851663194285
50	22.089175593696208	22.051155639487273	30.715109380483646	25.144559386332872
51	22.06016256087679	22.721766951524113	30.6597666006733	24.558303886925795
52	21.759475900795508	22.924326492412593	30.859348886957683	24.456848719834213
53	23.856917825104144	21.559680943964636	31.830811721005638	22.752589509925585
54	22.297954389495352	21.584790323870788	30.2105391671894	25.906716119444457
55	23.468355488671516	21.799598695761226	28.39311094390101	26.338934871666247
56	22.842359549622596	24.15010908361405	26.606789096653934	26.40074227010942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	8.5
4	26.0
5	35.0
6	260.0
7	485.0
8	1035.0
9	1585.0
10	985.0
11	385.0
12	398.0
13	401.0
14	391.0
15	623.0
16	855.0
17	1056.5
18	1258.0
19	1404.5
20	1551.0
21	1825.0
22	2550.5
23	3002.0
24	3391.0
25	3780.0
26	4540.5
27	5301.0
28	5429.0
29	5693.0
30	5829.0
31	4939.0
32	4049.0
33	4368.0
34	4687.0
35	4006.5
36	3326.0
37	3358.5
38	3545.5
39	3700.0
40	3934.5
41	4169.0
42	4755.0
43	5341.0
44	5415.0
45	5489.0
46	5691.5
47	6552.0
48	7210.0
49	7808.5
50	8407.0
51	9246.0
52	10085.0
53	11527.0
54	13957.5
55	14946.0
56	16778.5
57	18611.0
58	19638.0
59	20665.0
60	20107.5
61	19550.0
62	17248.0
63	13286.0
64	11626.0
65	10176.0
66	8726.0
67	7626.0
68	6526.0
69	5658.0
70	4790.0
71	4164.0
72	3073.5
73	2609.0
74	2068.0
75	1527.0
76	1241.0
77	955.0
78	771.5
79	463.5
80	339.0
81	245.0
82	151.0
83	119.0
84	87.0
85	61.0
86	35.0
87	24.5
88	9.0
89	4.0
90	4.5
91	5.0
92	4.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.030227868438378627
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	8.28160779133661E-4
21	0.0
22	0.0
23	0.0
24	0.009523848960037102
25	0.02608706454271032
26	0.07453447012202949
27	0.07287814856376217
28	0.10973130323521008
29	0.17391376361806882
30	0.26128472581667006
31	0.3138729352916575
32	0.30766172944815506
33	0.3291939097056302
34	0.35362465269007326
35	0.3925482093093553
36	0.4331280874869047
37	0.44969130306957794
38	0.4894430204679936
39	0.5354059437099119
40	0.6194642627919784
41	0.6364415587642185
42	0.6559033370738595
43	0.691514250576607
44	0.6939987329140079
45	0.7229843601836861
46	0.7639783187508022
47	0.8066285988761859
48	0.8567323260137724
49	0.8240199752379928
50	0.8906869179582524
51	0.8617012906885743
52	0.8923432395165197
53	0.8977262845808884
54	1.6422428250220495
55	0.9436892078228067
56	0.9246415099027325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	241499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.29209230680044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.14341503519861	27.859328610056355
2	12.713632392991112	10.24517699866252
3	5.935974513128821	7.1751849904140395
4	3.4212013771132006	5.513894467471915
5	2.0769744617440007	4.184282336572822
6	1.404861004059401	3.396287355227144
7	0.9896716509942962	2.7913159060700043
8	0.6484764400596064	2.0902778065333605
9	0.5857869585324496	2.1242323984778406
>10	2.812805097374236	20.46054020927623
>50	0.16443142695647706	4.620308986786695
>100	0.09454807049997431	6.4608963184112564
>500	0.004110785673911926	0.9950351761290936
>1k	0.004110785673911926	2.0832384399107244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1398	0.578884384614429	No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1285	0.5320933005933771	No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1267	0.5246398535811743	No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	1081	0.44762090112174374	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	683	0.2828169060741452	No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	612	0.2534171984149003	No Hit
GTAATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG	597	0.2472059925713978	No Hit
GTAATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	511	0.21159507906865038	No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	422	0.17474192439720246	No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	395	0.16356175387889804	No Hit
GTAATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	369	0.15279566375016046	No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.1482407794649253	No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	357	0.14782669907535848	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	324	0.13416204621965308	No Hit
GTAATGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCC	305	0.1262945188178833	No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	269	0.1113876247934774	No Hit
GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	265	0.10973130323521008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.140803895668305E-4	0.0	0.0	0.0	0.0
8	0.0020704019478341527	0.0	0.0	0.0	0.0
9	0.0037267235061014745	0.0	0.0	0.0	0.0
10	0.007453447012202949	0.0	0.0	0.0	0.0
11	0.015320974413972729	0.0	0.0	0.0	0.0
12	0.018633617530507372	0.0	0.0	0.0	0.0
13	0.019461778309641034	0.0	0.0	0.0	0.0
14	0.02484482337400983	0.0	0.0	0.0	0.0
15	0.02525890376357666	0.0	0.0	0.0	0.0
16	0.02608706454271032	0.0	0.0	0.0	0.0
17	0.028571546880111306	0.0	0.0	0.0	0.0
18	0.03147010960707912	0.0	0.0	0.0	0.0
19	0.03436867233404693	0.0	0.0	0.0	0.0
20	0.038095395840148406	0.0	0.0	0.0	0.0
21	0.04140803895668305	0.0	0.0	0.0	0.0
22	0.045548842852351355	0.0	0.0	0.0	0.0
23	0.05341637025412114	0.0	0.0	0.0	0.0
24	0.05962757609762359	0.0	0.0	0.0	0.0
25	0.06418246038285873	0.0	0.0	0.0	0.0
26	0.06873734466809386	0.0	0.0	0.0	0.0
27	0.07163590739506168	0.0	0.0	0.0	0.0
28	0.0840583190820666	0.0	0.0	0.0	0.0
29	0.08778504258816806	0.0	0.0	0.0	0.0
30	0.09441032882123736	0.0	0.0	0.0	0.0
31	0.097722971937772	0.0	0.0	0.0	0.0
32	0.1018637758334403	0.0	0.0	0.0	0.0
33	0.10517641894997495	0.0	0.0	0.0	0.0
34	0.10890314245607642	0.0	0.0	0.0	0.0
35	0.12132555414308134	0.0	0.0	0.0	0.0
36	0.1262945188178833	0.0	0.0	0.0	0.0
37	0.13002124232398476	0.0	0.0	0.0	0.0
38	0.13581836777792042	0.0	0.0	0.0	0.0
39	0.13954509128402187	0.0	0.0	0.0	0.0
40	0.14285773440055652	0.0	0.0	0.0	0.0
41	0.14617037751709117	0.0	0.0	0.0	0.0
42	0.1494830206336258	0.0	0.0	0.0	0.0
43	0.1548660656979946	0.0	0.0	0.0	0.0
44	0.16107727154149706	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAAGCA	15	0.00801044	50.639065	49
TAACCGA	15	0.00801044	50.639065	50
ACAGATA	15	0.00801044	50.639065	49
CGATACA	15	0.008239745	50.27887	46
CGTGTTA	15	0.008253383	50.257843	44
ACAGGTA	15	0.008260209	50.247337	43
CCGATTG	20	4.07673E-4	50.247334	43
CCCGATT	20	4.07673E-4	50.247334	42
ACCCGAT	20	4.080939E-4	50.236835	41
GTCTACG	15	0.008287555	50.205345	40
CGGTAAG	15	0.008287555	50.205345	38
CTTCGTC	15	0.008294401	50.194862	37
CCGGTAA	15	0.008294401	50.194862	37
TACCATC	15	0.008308107	50.173904	36
CGCCCAA	15	0.008376891	50.069374	33
GTTAGAC	15	0.008383792	50.058945	32
CTAAGGC	15	0.008383792	50.058945	32
TACGGAC	15	0.008383792	50.058945	32
CATAAAC	15	0.008390698	50.04852	31
TTGTTAG	20	4.165842E-4	50.027687	30
>>END_MODULE