FastQCFastQC Report
Fri 17 Jun 2016
SRR1527018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393754
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC13580.34488538529132406No Hit
CCTAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT10920.27733051600745645No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9490.2410134246255276No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC6700.17015700157966648No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC5810.1475540565937108No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC4300.10920523981978594No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC4290.10895127414578644No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT4110.10437989201379543No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAATGTCC4030.1023481666217994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA150.004688929357.9875239
ATAGATT150.004700761357.95062331
GTTCATA150.00471261557.91377311
TATAACT202.0235627E-457.91377313
ATCATAC150.00472924857.86226323
GCATATA202.0324845E-457.86226324
GACTAAT202.0388763E-457.8255239
CTAGAGG394100.057.5086062
TAGAGGG393200.057.3814663
CCTAGAG396150.057.3289531
AGAGGGG293500.057.1921124
GAGGGGG172000.055.878135
GAGGGGA68950.055.8661885
GAGGGAT17550.055.823915
AGGGATT4900.055.769826
AGAGGGA71200.055.5298354
AGGGGGG96550.055.1921926
AGGGGAG36850.054.3562136
AGGGTAC3550.054.0484246
GAGGGGT22750.053.798255