FastQCFastQC Report
Fri 17 Jun 2016
SRR1527017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567482
Sequences flagged as poor quality0
Sequence length64
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC35860.6319143162250079No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC28150.4960509760662012No Hit
CCTAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT27550.48547795348574935No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT22210.3913780525197275No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC21040.3707606584878463No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC16810.2962208492956605No Hit
CCTAGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT13360.2354259694580621No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC13290.2341924501570094No Hit
CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG12060.212517753867083No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT11920.21005071526497757No Hit
CCTAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC11260.1984203904264805No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC11250.19824417338347297No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT10560.18608519741595328No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC9810.1728689191903884No Hit
CCTAGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT8510.14996070359940933No Hit
CCTAGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA8310.1464363627392587No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC8030.14150228553504782No Hit
CCTAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG7750.13656820833083694No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAATGTCC7660.13498225494376914No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCTCTTCCGAAGGTC7650.13480603790076162No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTCAGAGGTCC7470.13163413112662603No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC7380.13004817773955826No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG6860.12088489150316661No Hit
CCTAGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC6730.11859406994406871No Hit
CCTAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG6600.11630324838497079No Hit
CCTAGAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG6330.11154538822376746No Hit
CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCAGTGCCAG5810.10238210198737581No Hit
CCTAGAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCATTCAGC5710.1006199315573005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGT150.00465827358.0870137
ACGACGT150.00466807558.0561835
CTAGACC202.0051541E-458.02537531
TATACGC150.004742083657.82597712
CCTAGAG573000.057.1599161
CTAGAGG571800.057.1532942
TAGAGGG570900.057.1268353
AGAGGGG432100.056.8824544
GAGGGGG239250.055.859275
GAGGGGA113600.055.854675
AGGGGAG58400.055.191426
GAGGGAT24150.055.1112065
AGGGGGC54000.055.0805856
AGGGGGG123850.055.040016
GAGGGGT32250.054.9958535
AGGGGTC12300.054.8087656
AGAGGGA99800.054.5907444
AGGGGAT17750.054.280666
AGGGATT7300.053.9032946
GAGGGGC57750.053.6081735
Produced by FastQC (version 0.11.5)