FastQCFastQC Report
Fri 17 Jun 2016
SRR1527016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568487
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC33830.5950883661367806No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC26870.4726581258674341No Hit
CCTAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT26220.46122426722158993No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT19640.3454784366221215No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC19290.33932174350512856No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC16040.2821524502759078No Hit
CCTAGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT12780.2248072515290587No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC12280.21601197564764013No Hit
CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG11240.19771780181428952No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT11220.19736599077903277No Hit
CCTAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC11060.19455150249697883No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT10270.18065496660433747No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC10110.17784047832228353No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC9890.17397055693445937No Hit
CCTAGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT7970.14019669754981204No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC7500.1319291382212786No Hit
CCTAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG7340.12911464993922464No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTCAGAGGTCC7260.12770740579819767No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAATGTCC7190.1264760671747991No Hit
CCTAGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA6970.12260614578697489No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCTCTTCCGAAGGTC6920.12172661819883304No Hit
CCTAGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC6600.11609764163472516No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG6550.11521811404658329No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC6540.11504220852895491No Hit
CCTAGAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG6090.10712646023567822No Hit
CCTAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT5990.10536740505939449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAT150.004556701558.4113326
ATATACC150.004668596758.05453555
GAAACGT202.013344E-457.97754737
AACGTTG150.00478919357.6817711
CTAGAGG573200.056.9969832
TAGAGGG571200.056.973863
CCTAGAG575600.056.8750461
AGAGGGG434650.056.681724
GAGGGGA113150.056.107255
GAGGGGG238150.055.7796945
AGGGGGG124000.055.137956
AGAGGGA98950.055.131184
GAGGGAT25400.054.973655
AGGGGAG59900.054.971566
AGGGGGC53850.054.597966
AGGGGAT17700.054.3891336
GAGGGGT33100.054.325485
GAGGGGC59200.054.1565555
AGGGGAC26800.054.151456
CAACTAG700.053.5616416
Produced by FastQC (version 0.11.5)