FastQCFastQC Report
Fri 17 Jun 2016
SRR1527015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522123
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC59001.1300019344100911No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC38970.7463758539654449No Hit
CCTAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG29010.5556162053768939No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC22330.4276770033114803No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC16060.3075903570614587No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC15360.2941835544498136No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT14270.27330724752596613No Hit
CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC13080.25051568308616934No Hit
CCTAGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC11340.21719020230865138No Hit
CCTAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG10300.1972715241427786No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT9490.18175793826358924No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC9100.17428843395138693No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT9100.17428843395138693No Hit
CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG8440.1616477343461215No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTC7580.14517651970895748No Hit
CCTAGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC7090.13579175788080586No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC6900.13215276860050218No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC6590.1262154703010593No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCTCTTC6590.1262154703010593No Hit
CCTAGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT6420.12295953252394551No Hit
CCTAGAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT5730.1097442556638953No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5640.10802052389954091No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5610.10744594664475612No Hit
CCTAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC5530.10591374063199667No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA5320.10189169984850313No Hit
CCTAGAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA5300.10150864834531327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGC150.008268164550.24340445
TACTACC150.008268164550.24340444
TATACTA204.0827435E-450.24340444
CGTACTA150.00827132450.2385542
GCGTACT150.00829980250.1948841
CGCGTAC150.00830931150.18034440
ACGCGTA150.00831882850.16581739
ATACGTG252.0465937E-550.16097638
GTACCAT150.00832835450.15129537
GATACGT252.0489319E-550.1512937
CGAATCG150.00834106750.13194736
CGATACG252.0536147E-550.13194736
CAAGGTA150.00835061150.11744334
TCGCTTA150.00835061150.11744334
CACATAG252.0677166E-550.0739932
GCGTATG150.00837929350.0739932
AATCGCT150.00837929350.0739932
ACTATCC150.00839845450.04506330
TATCGTG150.00839845450.04506330
ATATCGT150.00842405350.00654229
Produced by FastQC (version 0.11.5)