FastQCFastQC Report
Fri 17 Jun 2016
SRR1527014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362882
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC20950.5773226558495599No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC11870.32710357636917786No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC9470.26096637474440726No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC9290.2560060846225495No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT7580.20888332846490046No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC5300.14605298692136837No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC3650.1005836608043386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG150.004517119458.53584358
CTAAGGT150.004517119458.53584356
AAGACTC150.004689149657.9859139
TATTATG150.00470455957.93787833
ATATCGC150.004722585457.88194713
ATCGGTA150.004722585457.88194716
TTCTGGG360950.057.1633722
TCTGGGG350600.057.141693
CTTCTGG366500.056.8232081
CTGGGGG243150.056.539814
TGGGGGA48050.055.454525
TGGGGAC14050.055.280235
TGGGGGG141400.055.1958165
CTGGGGA71450.054.5553634
CTGGGGT18350.054.057164
TGGGGGT23900.053.6754385
TGGGGGC37200.053.643375
TGGGGTA3600.052.522555
CTGGGGC26900.051.9067544
TGGGGCA10400.051.7455835