FastQCFastQC Report
Fri 17 Jun 2016
SRR1527013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499427
Sequences flagged as poor quality0
Sequence length64
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC50271.00655351032283No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC38740.7756889395246953No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC31910.6389322163199026No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT31240.6255168423012772No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC24920.49897182170767707No Hit
CTTCTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG19650.3934508947253552No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC18840.37723230822522613No Hit
CTTCTGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG17920.3588111976324868No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT15440.3091542908172766No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC12640.2530900411872005No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC12390.2480843046130866No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT8730.1748003211680586No Hit
CTTCTGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC7180.14376475440855221No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC7110.1423631481678003No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT6740.13495465803811169No Hit
CTTCTGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT6520.1305496098528914No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTC5750.11513194120462049No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC5630.1127291876490458No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT5550.11112735194532934No Hit
CTTCTGGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGC5500.11012620463050657No Hit
CTTCTGGGGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5470.10952551624161287No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA5370.10752322161196731No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC5310.10632184483417997No Hit
CTTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG5210.10431955020453439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT201.9742832E-458.20639458
GATAATC150.004622078558.20052354
CAATTCA150.004622078558.20052354
CGCTAAT150.004622078558.20052356
ACGCTAA150.004622078558.20052355
CCGTAAT201.9821648E-458.15950447
CGTAATC201.9821648E-458.15950448
GTAATCC201.9841391E-458.14779349
ACGTCAA202.0109452E-457.99016629
ATAGGGT150.00471309957.9145724
CTACGTT150.004731837757.85655621
TCTGGGG487600.056.7579963
TTCTGGG501400.056.6676672
CTTCTGG506450.056.6458051
CTGGGGG343900.056.407094
TGGGGGG189050.055.813165
TGGGGGA71850.055.624175
CTGGGGA90950.055.1750834
TGGGGGT34100.053.975835
TGGGGAC20100.053.704345
Produced by FastQC (version 0.11.5)