FastQCFastQC Report
Fri 17 Jun 2016
SRR1527012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501519
Sequences flagged as poor quality0
Sequence length64
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC47520.9475214298959761No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC35710.7120368321040679No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC29060.5794396623059146No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT26590.5301892849523149No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC24270.48392982120318473No Hit
CTTCTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG18210.36309691158261204No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC17660.3521302283662234No Hit
CTTCTGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG17140.3417617278707287No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT14990.29889196620666414No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC12500.24924280037246843No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC11560.23049974178445878No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT8690.17327359481894006No Hit
CTTCTGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC6880.13718323732500665No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC6830.13618626612351675No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT6350.12661534258921395No Hit
CTTCTGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT6010.11983593841908283No Hit
CTTCTGGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGC5690.11345532272954764No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC5520.11006562064448207No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT5420.10807167824150232No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA5180.10328621647435092No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTC5150.10268803375345699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCA303.5480298E-758.4830245
ATTAACG150.004570703558.36506722
TCGCGAA303.7381142E-758.0431357
CGCGAAT258.624347E-658.04312558
ATTAGTA150.004673764658.03730447
ACGGTAT150.00469793557.96174238
AACTAGT150.004722198557.8863834
GGTAATC150.004750310457.799668
ATTCGAG150.004782321457.70169413
TTCTGGG502450.056.603092
TCTGGGG489750.056.565863
CTTCTGG508900.056.4487041
CTGGGGG340550.056.2833824
TGGGGGG185150.055.625535
TGGGGGA71200.055.2535485
CTGGGGA92600.054.9081574
TGGGGGT31700.054.6197365
TGGGGGC59000.054.283745
TGGGGAC17850.054.0866785
TGGGGAG35650.053.43285
Produced by FastQC (version 0.11.5)