FastQCFastQC Report
Fri 17 Jun 2016
SRR1527011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461887
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC82631.7889656994026677No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC47251.0229774815052166No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC31720.686748057425301No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC20550.4449140157657609No Hit
CTTCTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC19080.41308804967448753No Hit
CTTCTGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT16530.3578797411488091No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG14900.3225897243265128No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC11980.25937079848534383No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT11620.25157668434054214No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC10040.21736918337169048No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA9280.20091494239933144No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT8760.18965677752350682No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC8130.17601707777010395No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC7490.16216087484601213No Hit
CTTCTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6330.1370465070460957No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC6330.1370465070460957No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC6320.13683000387540675No Hit
CTTCTGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC6110.13228343729093914No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5440.1177777248547805No Hit
CTTCTGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5430.11756122168409155No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC5330.1153961899772022No Hit
CTTCTGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC5180.1121486424168682No Hit
CTTCTGGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCC5120.11084962339273459No Hit
CTTCTGGGGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4790.10370501875999974No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCT4620.10002446485828785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTC150.0082661350.245645
ACGTACT150.00829114950.20720341
CTTATAG150.00842070250.01065430
ATACAAC150.00844606849.9726228
CAACATA402.7030183E-949.94548425
CGTGTAA150.00852980949.84803821
GGTAATC150.00856641149.7940648
GCGTACT252.1384207E-549.7886779
GGTACGA150.00857007849.78867315
TTCTGGG463450.048.8646932
TCTGGGG451400.048.8564263
CTTCTGG467700.048.803971
CTGGGGG316300.048.4323274
CTGGGGA84100.048.1954464
TGGGGGG171600.047.8208775
TGGGGGA65500.047.5134245
TGGGGGC54800.047.1589785
TGGGGGT30200.046.908655
TGGGGAC18050.046.7595255
TGGGGAG33450.046.6679845
Produced by FastQC (version 0.11.5)