FastQCFastQC Report
Fri 17 Jun 2016
SRR1527010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717163
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT433016.037818459680714No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT24170.33702240634277003No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC18960.26437504444596277No Hit
GTTGCCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT18380.256287622200253No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT15010.20929691018638721No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT10090.14069325941243482No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9560.133303028739631No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC9350.13037482413342572No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAC7550.10527592750880903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCT150.004682549358.0123843
GCATAAC150.004738542757.838469
GGTCGTA202.0377572E-457.838469
TCGCGTA150.004738542757.8384619
CATGCAT62500.056.7300258
CGGGTAC108000.056.6183556
CCATGCA65450.056.5439657
ATTAAGC77700.056.3129551
AGATTAA78750.056.07043549
TAGCATA91850.056.0313830
GGTACCT106450.055.984238
GTAGCAT94150.055.9545529
GGGTACC107400.055.933917
GTACCTG106150.055.904159
GTTGATC103700.055.89291416
AGTAGCA93600.055.8925328
ACGATTA2650.055.87011748
GCCATGC71300.055.80572556
CAGTAGC94950.055.7991527
GCCAGTA95650.055.79896525