FastQCFastQC Report
Fri 17 Jun 2016
SRR1527009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042175
Sequences flagged as poor quality0
Sequence length64
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11006510.56108618993931No Hit
GTTGCCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT61650.5915513229543983No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC56270.5399285148847363No Hit
GTTGCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG44780.4296783169813131No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC38120.36577350253076496No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC29260.28075898961306883No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC23810.2284645093194521No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT21200.20342073068342648No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA21140.20284501163432245No Hit
GTTGCCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG19540.1874925036582148No Hit
GTTGCCGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG17660.16945330678628828No Hit
GTTGCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG17200.16503946074315734No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC15730.15093434404010844No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT15330.1470962170460815No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT13730.13174370906997385No Hit
GTTGCCGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC13030.12502698683042676No Hit
GTTGCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT12970.1244512677813227No Hit
GTTGCCGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG12700.12186053206035455No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC12370.11869407729028233No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCG12010.11523976299565811No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG11410.10948257250461774No Hit
GTTGCCGGGGCTGTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT11390.10929066615491641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT150.00466966758.0546337
GCACGTA150.004682136758.01553733
CGAATAT150.0046839258.00995332
TACGGTA150.004728669357.87079617
GAACGTA150.004728669357.8707969
GTACGGT150.004728669357.87079616
CATGCAT123950.056.48266658
CGGGTAC143350.056.4002046
ATTAAGC127150.056.1670151
GGGTACC142800.055.9279677
TAGCATA132350.055.87920430
AAAGATT129250.055.8364547
GGTACCT142600.055.8236478
CCATGCA125800.055.76764757
GTTGATC140300.055.74653216
ATATGCT132050.055.73094634
GTAGCAT133800.055.71794529
ACCTGGT142050.055.71159411
TTGATCC140200.055.7037417
CATATGC133000.055.70363633
Produced by FastQC (version 0.11.5)