FastQCFastQC Report
Fri 17 Jun 2016
SRR1527008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039557
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1038999.9945457536239No Hit
GTTGCCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT57460.5527354440401056No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC54220.5215683218909594No Hit
GTTGCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG40960.394013988650935No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC34340.33033301685237076No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC29010.2790611770205963No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC22220.21374489325741639No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA20840.20047000789759484No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT17380.167186599676593No Hit
GTTGCCGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG17120.16468553431894548No Hit
GTTGCCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG17080.16430075503315356No Hit
GTTGCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG16260.15641277967441902No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC14540.1398672703853661No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT14280.13736620502771854No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT12560.12082069573866562No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC12170.1170690977021943No Hit
GTTGCCGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG11980.11524139609468263No Hit
GTTGCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT11440.1100468757364916No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCG11420.10985448609359563No Hit
GTTGCCGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC10940.1052371346640925No Hit
GTTGCCGGGGCTGTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT10920.10504474502119653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA150.004663417658.0742550
ATACGAT150.004781583357.70833613
CATGCAT123100.056.4948658
CGGGTAC144300.056.3017466
ATTAAGC128050.056.14947551
CCAGTAG135150.056.0979926
GCCAGTA135350.056.06341625
TAGCATA133050.056.0159830
AAAGATT129900.056.00959847
GGTACCT141650.055.998098
TGCCAGT135700.055.89188424
AAGATTA129950.055.87634748
GGGTACC143200.055.866687
GTAGCAT134850.055.8473329
ATATGCT132400.055.8181834
GTTGATC139900.055.8108516
AGATTAA129550.055.8104549
CATATGC133750.055.7902633
TACCTGG141850.055.7759110
ACCTGGT141300.055.7683911
Produced by FastQC (version 0.11.5)