FastQCFastQC Report
Fri 17 Jun 2016
SRR1527007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966032
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG11405111.806130645775708No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC95730.9909609619557116No Hit
GTTGCCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC50280.5204796528479388No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC47400.49066697583516905No Hit
GTTGCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC35710.36965649171041953No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG35370.3661369395630787No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA23690.24522997167795682Illumina Single End Adapter 1 (95% over 21bp)
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC22400.23187637676598702No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA17970.186018682610928No Hit
GTTGCCGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC16690.1727686039385859No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT16500.1708017953856601No Hit
GTTGCCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT16380.16955960051012803No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC16270.1684209218742236No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC14590.15103019361677458No Hit
GTTGCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC14160.14657899531278468No Hit
GTTGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA13750.1423348294880501No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT13640.14119615085214568No Hit
GTTGCCGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT12400.12836013713831426No Hit
GTTGCCGGGGCTGTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTC11160.11552412342448284No Hit
GTTGCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC10810.1119010550375143No Hit
GTTGCCGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC10390.10755337297315203No Hit
GTTGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC9940.10289514218990675No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT9920.10268810971065141No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC9830.10175646355400235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG150.00800696550.656450
CGACTAA150.00834889850.12331437
TATATGC252.074843E-550.05030433
CGCATAT150.00841095950.02948431
TCGCATA150.008417875550.01907730
CCGATAG301.0706917E-649.84287321
ACATACG301.077211E-649.79901517
GAGATAC150.00856585949.7990159
GATTAAG128700.048.92455750
AGATTAA129500.048.69286349
AAGATTA129900.048.66401748
AAAGATT129550.048.5903647
GTCTCAA129900.048.49373642
TCTCAAA129850.048.4376343
CGGGTAC147500.048.386676
CTCAAAG130400.048.3806844
TGTCTCA130250.048.34829341
CAAAGAT130600.048.20886646
TCAAAGA130600.048.184645
ATATGCT132050.048.1827534
Produced by FastQC (version 0.11.5)