FastQCFastQC Report
Fri 17 Jun 2016
SRR1527006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65241
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3760.5763247037905612No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA2330.3571373829340445No Hit
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.2759001241550559No Hit
CACAAGGGGAGGTGATTGGTACCATCCTGTTTTCTCATCTCCCTCCCATTTCCTCTTCCCTGTT1190.1824006376358425No Hit
CACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.1701384098956178No Hit
CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.15174506828528073No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA830.12722061280483132No Hit
CACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.11802394199966278No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.11036004966202234No Hit
CACAAGGGGGATCATGGGCAATTCATTTAATTGCCTGCCAATTATAGACTATATGGGGGACCAA680.10422893579191No Hit
CACAAGGGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCT670.1026961573243819No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCAA150.00452968858.4399757
AATCAAA150.00452968858.4399758
GCTAATT150.00452968858.4399757
ACAAGAG453.092282E-1158.124812
GGGATGT201.975109E-458.124817
GGGATGA258.469586E-658.124817
AAATATA201.9978108E-457.9907835
AATATAA201.9978108E-457.9907836
ATACTAT201.9978108E-457.9907845
TTATACT201.9978108E-457.9907843
ATTATAC201.9978108E-457.9907842
ACTATTA258.586487E-657.99077647
TACTATT258.586487E-657.99077646
AAGCGCA150.00467035857.99077251
GTAATGG150.00467035857.99077232
TAGGCTT150.00467035857.99077235
CACAAAT150.00467035857.99077232
TAATGGG150.00467035857.99077233
TGGTAGG150.00467035857.99077231
TACACAA202.0054239E-457.9462430
Produced by FastQC (version 0.11.5)