FastQCFastQC Report
Fri 17 Jun 2016
SRR1527005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96355
Sequences flagged as poor quality0
Sequence length64
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9450.980748274609517No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA4090.424472004566447No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3140.32587826267448494No Hit
CACAAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2910.30200819884801No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2580.26775984640132844No Hit
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.25323024233303926No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2180.2262466919205023No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2080.21586840330029577No Hit
CACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1910.19822531264594467No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1850.19199833947382078No Hit
CACAAGGGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCT1730.17954439312957293No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1710.17746873540553162No Hit
CACAAGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1690.17539307768149034No Hit
CACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.17227959109542837No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1630.1691661045093664No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1590.16501478906128378No Hit
CACAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1540.15982564475118052No Hit
CACAAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT1540.15982564475118052No Hit
CACAAGGGGAGGTGATTGGTACCATCCTGTTTTCTCATCTCCCTCCCATTTCCTCTTCCCTGTT1530.15878781588915986No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1470.15256084271703596No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1430.14840952726895335No Hit
CACAAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1400.1452960406828914No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGAGCACTGACTGTTCTTCCAAAGGTCC1400.1452960406828914No Hit
CACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1290.13387992320066422No Hit
CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.13387992320066422No Hit
CACAAGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC1230.1276529500285403No Hit
CACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1220.12661512116651963No Hit
CACAAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG1140.11831249027035441No Hit
CACAAGGGGCAGACTCCTAGTCACAGCTGGAGTAATGGGCACACAGAAAGCATCATTTCAGAAA1120.1162368325463131No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1110.11519900368429246No Hit
CACAAGGGGAGGTGATGTTTTCACTCTGGTTGTTTGCATCCTTCCCTGCTTGTGTTCCCAGCAC1080.11208551709823049No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1080.11208551709823049No Hit
CACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1070.11104768823620985No Hit
CACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.11104768823620985No Hit
CACAAGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1070.11104768823620985No Hit
CACAAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1060.1100098593741892No Hit
CACAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1060.1100098593741892No Hit
CACAAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1040.10793420165014789No Hit
CACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1030.10689637278812725No Hit
CACAAGGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGCCTACCCAC1030.10689637278812725No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1030.10689637278812725No Hit
CACAAGGGGGATCATGGGCAATTCATTTAATTGCCTGCCAATTATAGACTATATGGGGGACCAA1020.10585854392610658No Hit
CACAAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGAC1010.10482071506408594No Hit
CACAAGGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG1000.10378288620206527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGG150.004593902358.25418557
TGTTTAT150.004593902358.25418556
CGGAACT150.004593902358.25418558
TCGTTGC150.004593902358.25418558
TCGGAAC150.004593902358.25418557
ACTATTA351.5716068E-858.22373247
ACTATAT201.9633053E-458.22373248
CTATATG150.004603428858.22373249
ATTAACC150.004603428858.22373249
ATATGGG150.004603428858.22373251
TAACCAC150.004603428858.22373251
TATTAAC150.004603428858.22373248
TCCAAGC303.6273195E-758.22373251
ACGGTAA150.004603428858.22373251
GCATCCC150.004612970658.1933145
TTAGAAA150.004622526558.1629242
GTTGTCG150.004622526558.1629241
ATTATAC351.5845217E-858.1629242
CAACTAA150.004622526558.1629239
GATACGT150.004641683358.1022437
Produced by FastQC (version 0.11.5)