FastQCFastQC Report
Fri 17 Jun 2016
SRR1527004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97054
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9220.9499866053949347No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA3710.38226142147670367No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3140.32353123003688666No Hit
CACAAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2840.2926206029632988No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2490.25655820471077956No Hit
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.24213324540977185No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2130.21946545222247407No Hit
CACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2000.20607084715725268No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1970.2029797844498939No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1900.19576730479939No Hit
CACAAGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1820.18752447091309993No Hit
CACAAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1720.17722092855523727No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1720.17722092855523727No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1570.16176561501844333No Hit
CACAAGGGGAGGTGATTGGTACCATCCTGTTTTCTCATCTCCCTCCCATTTCCTCTTCCCTGTT1560.16073526078265707No Hit
CACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.15867455231108454No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1490.15352278113215323No Hit
CACAAGGGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCT1450.14940136418900818No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGAGCACTGACTGTTCTTCCAAAGGTCC1310.1349764048880005No Hit
CACAAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT1300.13394605065221424No Hit
CACAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1240.12776392523749666No Hit
CACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.12570321676592414No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1210.12467286253013787No Hit
CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.1236425082943516No Hit
CACAAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG1180.12158179982277907No Hit
CACAAGGGGAGGTGATGTTTTCACTCTGGTTGTTTGCATCCTTCCCTGCTTGTGTTCCCAGCAC1180.12158179982277907No Hit
CACAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1170.12055144558699282No Hit
CACAAGGGGCAGACTCCTAGTCACAGCTGGAGTAATGGGCACACAGAAAGCATCATTTCAGAAA1150.11849073711542028No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1140.117460382879634No Hit
CACAAGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC1120.11539967440806148No Hit
CACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1110.11436932017227523No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1100.11333896593648896No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1080.11127825746491643No Hit
CACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1070.11024790322913018No Hit
CACAAGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1030.10612648628598512No Hit
CACAAGGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG1030.10612648628598512No Hit
CACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1010.10406577781441259No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1000.10303542357862634No Hit
CACAAGGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGCCTACCCAC990.10200506934284007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAT351.5195837E-858.4726245
TAATTAT351.5195837E-858.4726240
TACTAAC258.205079E-658.4726240
ATTATAC351.5195837E-858.4726242
TAGGATG258.205079E-658.4726243
GTATTTT150.00452637258.47261443
CGTATTT150.00452637258.47261442
CCCTCAA150.00452637258.47261439
GTGGCGT201.9223636E-458.47261439
GTGTCAA150.00452637258.47261444
GCGTATT150.00452637258.47261441
CTGATTG150.004563908658.35085726
ATCTCTA258.332903E-658.320525
TACTATC150.00457332958.320525
ATTTAAT201.9473021E-458.32049625
TCTGCAA150.004582763658.29017323
AGGTAAC150.004582763658.29017324
ACGCATA150.004582763658.29017323
CGCATAG150.004582763658.29017324
GTTACTA150.004582763658.29017323
Produced by FastQC (version 0.11.5)