FastQCFastQC Report
Fri 17 Jun 2016
SRR1527003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88415
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG10121.1446021602669232No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT3180.3596674772380252No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2870.3246055533563309No Hit
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2830.3200814341457897No Hit
CACAAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2790.3155573149352485No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2590.2929367188825425No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2400.2714471526324719No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2360.2669230334219307No Hit
CACAAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2250.25448170559294236No Hit
CACAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2120.23977831815868347No Hit
CACAAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1960.22168184131651866No Hit
CACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1890.21376463269807158No Hit
CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.1922750664480009No Hit
CACAAGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT1620.1832268280269185No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1600.1809647684216479No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1560.17644064921110672No Hit
CACAAGGGGAGGTGATGTTTTCACTCTGGTTGTTTGCATCCTTCCCTGCTTGTGTT1470.16626138098738902No Hit
CACAAGGGGAGGTGATTGGTACCATCCTGTTTTCTCATCTCCCTCCCATTTCCTCT1440.16286829157948313No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1330.15042696375049483No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1330.15042696375049483No Hit
CACAAGGGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAA1280.14477181473731832No Hit
CACAAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC1270.14364078493468302No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1200.13572357631623594No Hit
CACAAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1190.13459254651360064No Hit
CACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.13459254651360064No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGAGCACTGACTGTTCTTCC1150.13006842730305943No Hit
CACAAGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT1130.12780636769778883No Hit
CACAAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC1070.12102018888197703No Hit
CACAAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1060.11988915907934174No Hit
CACAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1060.11988915907934174No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG1040.11762709947407113No Hit
CACAAGGGGAGTTAAGTTTTGTTTTTCTTCCCTATGATATCTTAGAAATAATTGTC1030.11649606967143583No Hit
CACAAGGGGAGGCAGAAGCTGCTATCTGATTGGTATAGCATCTGACTTTCCCTCTG1030.11649606967143583No Hit
CACAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA1020.11536503986880053No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1020.11536503986880053No Hit
CACAAGGGGCAGACTCCTAGTCACAGCTGGAGTAATGGGCACACAGAAAGCATCAT950.10744783125035345No Hit
CACAAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA950.10744783125035345No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG920.10405474184244756No Hit
CACAAGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC920.10405474184244756No Hit
CACAAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC900.10179268223717695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAAG150.00800748750.61666548
TTACAGG150.00800748750.61666548
AGGGTTA150.00828406350.18404444
TGAATGG150.00828406350.18404446
CGCCAAA150.00828406350.18404445
AAGAGTT150.0083027550.15546442
TCACAAT507.2759576E-1250.15546441
ATAATGA150.0083027550.15546442
TGTGAAG150.0083027550.15546440
ACTAGGG150.0083027550.15546441
ACTAACC252.0339166E-550.15546441
TACTAGG150.0083027550.15546440
TACTAAC252.0339166E-550.15546440
TCTATTT150.0083027550.15546440
TCTGAGA150.0083214750.1269239
GTTCTAT150.0083214750.1269238
GTACTAG150.0083214750.1269239
GCCTACT252.0476766E-550.09840837
CCGAGTA150.00835900150.06992335
AGGATTT204.1340844E-450.06992335
Produced by FastQC (version 0.11.5)