##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527003_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88415 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.728304020810945 39.0 38.0 40.0 36.0 40.0 2 38.426352994401405 39.0 38.0 40.0 35.0 40.0 3 38.42498444834021 39.0 38.0 40.0 35.0 40.0 4 38.27976022168184 39.0 38.0 40.0 35.0 40.0 5 38.36618220890121 39.0 38.0 40.0 35.0 40.0 6 38.23362551603235 39.0 38.0 40.0 35.0 40.0 7 38.788531357801276 39.0 39.0 40.0 38.0 40.0 8 38.74646835944127 39.0 39.0 40.0 37.0 40.0 9 38.73728439744387 39.0 39.0 40.0 37.0 40.0 10 37.899394899055594 39.0 38.0 40.0 35.0 40.0 11 37.64430243736923 39.0 37.0 40.0 33.0 40.0 12 37.64891703896398 39.0 37.0 40.0 34.0 40.0 13 37.57848781315388 39.0 37.0 40.0 34.0 40.0 14 37.88287055363909 39.0 38.0 40.0 34.0 40.0 15 37.86118871232257 39.0 38.0 40.0 34.0 40.0 16 37.647062150087656 39.0 38.0 40.0 33.0 40.0 17 37.530475598032005 39.0 37.0 40.0 33.0 40.0 18 37.38694791607759 39.0 37.0 40.0 33.0 40.0 19 37.30602273369903 39.0 37.0 40.0 33.0 40.0 20 37.28056325284171 39.0 37.0 40.0 33.0 40.0 21 37.06158457275349 39.0 36.0 40.0 33.0 40.0 22 36.92908443137477 39.0 36.0 40.0 32.0 40.0 23 36.79806593903749 39.0 36.0 40.0 32.0 40.0 24 36.5970367019171 39.0 36.0 40.0 31.0 40.0 25 36.414477181473735 38.0 36.0 40.0 31.0 40.0 26 35.89487077984505 38.0 35.0 40.0 30.0 40.0 27 35.64349940620935 38.0 35.0 40.0 29.0 40.0 28 35.333099587174125 38.0 35.0 40.0 29.0 40.0 29 35.22619465022903 38.0 35.0 40.0 29.0 40.0 30 34.82930498218628 38.0 34.0 39.0 28.0 40.0 31 34.06599558898377 37.0 33.0 39.0 25.0 40.0 32 33.929502912401745 37.0 33.0 39.0 24.0 40.0 33 33.60190013006843 37.0 33.0 39.0 23.0 40.0 34 33.691590793417404 37.0 33.0 39.0 23.0 40.0 35 33.40327998642764 36.0 33.0 39.0 23.0 40.0 36 33.15173895832155 37.0 33.0 39.0 20.0 40.0 37 32.899157382797036 36.0 32.0 39.0 19.0 40.0 38 32.659808855963355 36.0 32.0 39.0 17.0 40.0 39 32.4650229033535 36.0 32.0 39.0 15.0 40.0 40 32.097528699881245 36.0 31.0 39.0 10.0 40.0 41 31.577933608550584 36.0 31.0 39.0 4.0 40.0 42 31.310693886783916 36.0 30.0 39.0 4.0 40.0 43 31.093468302889782 35.0 30.0 39.0 4.0 40.0 44 30.802363852287506 35.0 30.0 39.0 4.0 40.0 45 30.430990216592207 35.0 29.0 38.0 4.0 40.0 46 29.64239099700277 35.0 28.0 38.0 4.0 40.0 47 29.306418594129955 35.0 28.0 38.0 4.0 40.0 48 28.988881977040094 34.0 27.0 38.0 4.0 40.0 49 28.707821071085224 34.0 27.0 38.0 4.0 39.0 50 28.228592433410622 34.0 25.0 38.0 4.0 39.0 51 27.280710286716054 33.0 21.0 37.0 4.0 39.0 52 26.914595939603007 33.0 17.0 37.0 4.0 39.0 53 26.608154724877 33.0 10.0 37.0 4.0 39.0 54 26.17904201775717 33.0 4.0 36.0 4.0 39.0 55 25.74833455861562 33.0 4.0 36.0 4.0 39.0 56 15.110614714697732 18.0 4.0 22.0 4.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5368975136570953 1 2 -0.3940903963542297 1 3 -0.4906486279944602 1 4 -0.2503993127082893 1 5 -1.1007767185515007 1 6 -0.790562088594811 1 7 -0.6215598646042011 1 8 -0.515103742457633 1 9 -0.3945627851346458 1 10 -0.29406528424053135 1 11 -0.1840405848784883 1 12 -0.5707025949846596 1 13 -0.6041213558031657 1 14 -1.3031957362011255 1 15 -1.1843962822544611 1 16 -0.0254770334706933 1 17 -0.29053983729843225 1 18 -0.13144400724705463 1 19 -0.27832094613937386 1 20 -0.21916574925073462 1 21 0.2837440261653441 1 22 -0.9553673413331225 1 23 0.5247692029460254 1 24 0.35059972019607955 1 25 -0.3450143595433417 1 26 0.3108221876741055 1 27 -0.061923553964938094 1 28 0.668021896992947 1 29 1.0738175977209323 1 30 0.7064059377839627 1 31 0.5546516986921333 1 32 0.45853635397632786 1 33 1.2233948600889235 1 34 0.015247363132587566 1 35 -0.3505108995135444 1 36 1.3470046227504042 1 37 1.1026769952466253 1 38 1.5445791695083813 1 39 0.6841492511412781 1 40 1.8669078668998402 1 41 1.0724214551696356 1 42 1.6307303997346771 1 43 1.249111042615823 1 44 1.0279420479139034 1 45 2.1523385925681104 1 46 1.5294356343772897 1 47 2.111801447620252 1 48 1.9564183225443692 1 49 3.090857791697161 1 50 2.504622976647507 1 51 2.7739960276756257 1 52 2.6082951372173717 1 53 2.725387023978019 1 54 3.719383328003598 1 55 2.812189699252105 1 56 2.5536388582004452 2 1 -0.28491046626233896 2 2 -0.19744629862093177 2 3 -0.3312432703259276 2 4 -0.46279260090376084 2 5 -0.32008787545465367 2 6 -0.41655561229219273 2 7 -0.12126548721239061 2 8 -0.08869809041171095 2 9 -0.0598556906395018 2 10 -0.41976443054609547 2 11 -0.611329369099856 2 12 -1.0474467810311694 2 13 -0.10853701668040827 2 14 -0.18470883599506038 2 15 -0.3395331677416564 2 16 -0.4955977282013393 2 17 0.11702566167124928 2 18 -0.32440838758264334 2 19 -0.29303981573018945 2 20 -0.27082898151449797 2 21 -0.30015353050230686 2 22 -1.0664948067437834 2 23 -0.0180627075632529 2 24 -0.8874044010874087 2 25 -1.0457797407620646 2 26 -0.5007762815634536 2 27 -0.580910895737091 2 28 -0.1387193452796467 2 29 -0.1434329174395046 2 30 0.06569354449577247 2 31 -0.6832052338954426 2 32 -0.4393146910634158 2 33 -1.0614152664933556 2 34 -0.8597894340413959 2 35 -1.1107405138791577 2 36 -0.5242052683299647 2 37 0.012744702046745715 2 38 -0.2639283371151038 2 39 -0.18558875298000288 2 40 -0.23803767328267256 2 41 0.1126039691525591 2 42 -0.20780360085408134 2 43 0.7739859322243028 2 44 0.5051278000481396 2 45 1.2400630553293723 2 46 0.291431513093805 2 47 0.6862789277497754 2 48 0.6579196472426325 2 49 0.4695743062688429 2 50 -0.496260155527942 2 51 0.31506167383400197 2 52 0.31200429235425986 2 53 0.5871768385202607 2 54 0.4366338431640351 2 55 0.1694166642211954 2 56 -0.5867791576959327 3 1 -0.8231564735472929 3 2 -0.5633751302264258 3 3 -0.7628767811132633 3 4 -1.02331375528874 3 5 -1.2822621539640338 3 6 -0.7980350182882674 3 7 -0.7487521781622348 3 8 -0.5724470396972237 3 9 -0.9158377584456119 3 10 -1.1154775154438212 3 11 -1.7812649252780588 3 12 -1.10082307691237 3 13 -1.1058752066495856 3 14 -1.0161742324646568 3 15 -1.2142880010282937 3 16 -0.8029667391287703 3 17 -0.7858883198361752 3 18 -0.6604969277899855 3 19 -1.7379823766716314 3 20 -0.7041436354791912 3 21 -0.9242779350974288 3 22 -0.7113533105263556 3 23 -0.8723636321919983 3 24 -0.6457400693418194 3 25 -1.0094950671840337 3 26 -0.44165607982627364 3 27 -0.8098265583876909 3 28 -0.375900628552273 3 29 -0.1907851169351602 3 30 -1.8691621573815098 3 31 -1.0354047295524893 3 32 -1.1109313904189762 3 33 -1.224065868902997 3 34 -0.6284921518895317 3 35 -0.3823852322876746 3 36 -0.03457231792368276 3 37 -0.8713965139801516 3 38 -1.1337798361344404 3 39 -1.7189314259976562 3 40 -1.8974660846023674 3 41 -0.4821004298387557 3 42 -1.1101572013023855 3 43 -1.052856320507555 3 44 -1.6809187115797677 3 45 -1.3413322935078362 3 46 -1.8765427093774747 3 47 -1.4774924367781033 3 48 -1.335635970577485 3 49 -1.5300054079368195 3 50 -0.8125952634012954 3 51 -0.5837895449948007 3 52 -0.5895087365053655 3 53 -1.0517976641415494 3 54 -0.6304717886656093 3 55 -0.22242979136860797 3 56 -0.872013116788116 4 1 -0.184602256679824 4 2 -0.11759199769630868 4 3 -0.31709364858314615 4 4 -0.4329523095056089 4 5 -0.1256356479399372 4 6 0.04533847568763605 4 7 0.03438035195824796 4 8 0.04201079162807275 4 9 0.14440320540980878 4 10 -0.19981486484141442 4 11 -0.22704805780817594 4 12 -0.3658833178762251 4 13 -0.08177882110742019 4 14 0.20069323741486045 4 15 0.24354332427291325 4 16 0.4741416946061747 4 17 -0.19552687405304425 4 18 -0.09423186754901991 4 19 0.5029814787500584 4 20 0.4042900994605674 4 21 0.4130714624929297 4 22 0.6621406653772652 4 23 0.4408893798561948 4 24 0.7638984848750425 4 25 0.6170109569123525 4 26 1.1366571731857746 4 27 0.9251132006484539 4 28 0.4554246726525477 4 29 1.977889581860019 4 30 0.865777601654635 4 31 1.8079687644234141 4 32 1.7565384890990998 4 33 1.23376545639821 4 34 0.9498211011225166 4 35 0.9790605508448564 4 36 2.0015722603895654 4 37 1.0804107149355104 4 38 1.1794731759137562 4 39 1.9678155619541542 4 40 0.8977146382891981 4 41 1.578140534016665 4 42 1.8055054493000249 4 43 1.4652159686490691 4 44 1.6323343004684254 4 45 1.4899930076969845 4 46 1.8343006641165012 4 47 2.7152786475592485 4 48 2.182436318579139 4 49 2.5663801342318564 4 50 2.5488505197312357 4 51 1.5366923827160406 4 52 1.7719370466271656 4 53 1.6951902876656817 4 54 0.05627519928619762 4 55 -0.12604424919993207 4 56 0.031601341043209885 5 1 0.07569820442385122 5 2 0.14880693135322787 5 3 -0.1054321879257003 5 4 -0.016769057980987156 5 5 -0.28017975027520947 5 6 -0.1462426636846743 5 7 0.07870580190618881 5 8 0.17156604969738964 5 9 0.15317904952701866 5 10 -0.0358999459064151 5 11 -0.11965552471283303 5 12 -0.1632951875772548 5 13 -0.04637795839732206 5 14 -0.8104624966324394 5 15 -0.23674821224352627 5 16 -0.028758796246130203 5 17 0.08530013769766498 5 18 -0.5321316146857171 5 19 0.36271671599533306 5 20 0.3595184201507351 5 21 0.4611154904566419 5 22 0.7992818225987435 5 23 0.7163616392641998 5 24 -0.12900646827087314 5 25 0.7528134260481565 5 26 1.147515420991816 5 27 0.554891573398713 5 28 1.4356418376086708 5 29 0.7345458365082393 5 30 1.559664259396719 5 31 1.2585116768137183 5 32 1.3766485590083661 5 33 0.20401683227207457 5 34 0.18543020420150214 5 35 1.577602868262673 5 36 0.929283103763062 5 37 0.7208985923711779 5 38 0.5673952345185427 5 39 0.6442992745824441 5 40 1.4382471386610582 5 41 2.2944874029739744 5 42 1.3622509498206234 5 43 1.575880850398292 5 44 1.047476350148628 5 45 2.461136842294632 5 46 1.7164961125770084 5 47 1.6740768031445512 5 48 1.2450571723646533 5 49 2.2233784980575315 5 50 2.082838322795343 5 51 1.701797246616085 5 52 1.9081857451248574 5 53 2.3271998072254014 5 54 2.1450748423027086 5 55 2.121613046650136 5 56 0.8849406241013682 6 1 -0.1341783387860218 6 2 -0.3697115871652912 6 3 -0.06839515088866222 6 4 -0.43652214440074033 6 5 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2.8029158656634294 6 47 2.8222249512927 6 48 3.208020135327615 6 49 2.1986871400328383 6 50 3.26802350798053 6 51 3.022784900937072 6 52 3.068679572285351 6 53 3.5617677084599677 6 54 2.947543978105177 6 55 3.0228476145513703 6 56 1.094817167311243 7 1 -0.45177093137861846 7 2 -0.08174862420232643 7 3 -0.07688280520964952 7 4 -0.10087399625259508 7 5 -0.016599503361625523 7 6 0.14187462026594488 7 7 0.0641996290666853 7 8 0.07002283982084379 7 9 0.26398151866282404 7 10 -0.013369081708880515 7 11 -0.3889456481696243 7 12 -0.3957025949846624 7 13 -0.6655137608664532 7 14 -0.3099995336694761 7 15 -0.07964944681142327 7 16 -0.05838842587575499 7 17 0.005979150043344816 7 18 -0.03429210851287223 7 19 -0.04129562968368106 7 20 -0.05745688849123809 7 21 -0.07021167003718887 7 22 0.2704237979073838 7 23 0.07176287383209967 7 24 0.6515490872846854 7 25 0.4295109569123525 7 26 0.27189813704119814 7 27 -0.16841089573708956 7 28 1.1191294919296553 7 29 0.1083112686070038 7 30 1.3008679631004227 7 31 0.910980812616188 7 32 0.7545806577737935 7 33 1.2260847335066458 7 34 1.0951524264237236 7 35 1.3130966954231695 7 36 1.304283103763062 7 37 0.4906516787909325 7 38 0.8811298024197782 7 39 1.182725200508365 7 40 1.043648373228958 7 41 1.0160923412455851 7 42 1.4073126782156855 7 43 0.3832882578056953 7 44 1.4565812884202352 7 45 1.2711677064921645 7 46 1.9678850014658984 7 47 2.083953346354427 7 48 1.9337917402658888 7 49 2.220446399292097 7 50 2.0473444956348494 7 51 2.987445394764233 7 52 2.9288647574705386 7 53 2.7113047454970065 7 54 1.597389657117521 7 55 3.631335268872359 7 56 1.6463603771877882 8 1 -0.15715067821405881 8 2 -0.2801663457213195 8 3 0.003022258081486484 8 4 -0.03520943929056841 8 5 0.1397296105624264 8 6 0.14614677216467697 8 7 0.16325026197807802 8 8 0.21907347273224076 8 9 0.14974101233370618 8 10 0.7312511714556749 8 11 0.4235543518303757 8 12 0.6065125948887555 8 13 0.6617014290069605 8 14 0.5195890739254594 8 15 0.4105404266062962 8 16 0.8099660045039911 8 17 0.6714854791572691 8 18 0.6027332079428191 8 19 0.7090208260125195 8 20 0.3884291874581294 8 21 0.7520984565450917 8 22 0.905392152337761 8 23 0.6007185700346298 8 24 1.363257948044179 8 25 1.2752388050136219 8 26 2.108290542104484 8 27 1.8494688510983508 8 28 1.971819365347379 8 29 1.4535644331639688 8 30 2.326659102340926 8 31 2.0862972683123857 8 32 2.724359138786454 8 33 1.4639011892028435 8 34 2.724108122626255 8 35 3.2064511258029142 8 36 4.233080572117494 8 37 3.7102719319554893 8 38 4.126857650521046 8 39 3.98794671949571 8 40 4.727667360570731 8 41 5.02178854377723 8 42 4.858578501000498 8 43 5.514933827425949 8 44 5.078574959306305 8 45 6.658667706492164 8 46 6.478802722984888 8 47 6.200409042556959 8 48 6.741228449126645 8 49 6.926300829671845 8 50 6.441331837407002 8 51 7.457540331473098 8 52 7.55766222582497 8 53 7.079817403724853 8 54 7.51685168243398 8 55 7.394468180264763 8 56 5.414398351871332 9 1 -0.16206504902567787 9 2 0.1814866699153157 9 3 -0.40114751109200597 9 4 0.21750835668858315 9 5 0.04516520252072809 9 6 -0.1015077326752305 9 7 -0.007859194462731978 9 8 0.11855225158554816 9 9 0.08898151866281978 9 10 -0.061898493473592 9 11 0.09414258712449453 9 12 0.32341505207416077 9 13 0.42566270972177733 9 14 0.4819122310364037 9 15 0.7129976120121029 9 16 0.8710233388301276 9 17 0.6662732676904 9 18 0.8289431856047713 9 19 0.5307631938457291 9 20 0.25871958209699386 9 21 0.7908177417275155 9 22 0.44910026849562 9 23 0.9313216973615113 9 24 0.603019675519981 9 25 1.099363898088825 9 26 1.3968981370411981 9 27 1.7955596924982018 9 28 1.7331000801649523 9 29 1.8215465627246488 9 30 1.639838551335714 9 31 2.0256866949691243 9 32 2.342080657773799 9 33 1.485643557036056 9 34 1.8782406617178395 9 35 2.302802577776106 9 36 1.7410478096454156 9 37 2.2935928552615223 9 38 1.9509827435962492 9 39 1.4643428475671882 9 40 2.3002660202877827 9 41 1.9190335177161693 9 42 1.286724442921571 9 43 2.1597588460409938 9 44 2.342610700184938 9 45 2.141020647668636 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0.6154275427350235 120 49 0.9764649178106168 120 50 0.2927148660052197 120 51 1.4055009503197908 120 52 1.5131240167297975 120 53 1.6605331405587336 120 54 1.2932229904508539 120 55 1.7635883552921108 120 56 0.7244467969408745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 36.0 5 0.0 6 24.0 7 20.0 8 41.0 9 49.0 10 52.0 11 113.0 12 101.0 13 171.0 14 219.0 15 305.0 16 424.0 17 524.0 18 693.0 19 784.0 20 787.0 21 983.0 22 945.0 23 1036.0 24 1288.0 25 1438.0 26 1452.0 27 1665.0 28 1900.0 29 2182.0 30 2521.0 31 2742.0 32 3289.0 33 4196.0 34 5402.0 35 7561.0 36 10308.0 37 13353.0 38 14197.0 39 7614.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.33156138664255 0.62319742125205 0.016965447039529492 0.028275745065882485 8 99.63455750910778 0.3156608512660376 0.027153621614282804 0.02262801801190234 9 98.86331504835152 0.6899281796075326 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18.848611660917264 23.040208109483686 28.224848724763895 23 30.86806537352259 20.86071367980546 22.312955946389188 25.95826500028276 24 29.17915596828377 21.114366184438236 23.75776221877863 25.948715628499365 25 29.239733001470753 21.61330467247426 21.163027491797713 27.98393483425727 26 28.574177410044253 18.924945387044563 23.377740551675743 29.12313665123544 27 26.253126450050363 18.51084779139646 23.235889948958228 32.00013580959495 28 26.737089999207452 22.06673233472595 22.952118927119777 28.24405873894682 29 31.18578015429756 20.30791539690272 21.73986926623693 26.76643518256279 30 27.906950380338053 19.93402183401163 26.41620659554931 25.742821190101004 31 29.68007530308357 20.18666999330891 24.94754868049491 25.1857060231126 32 33.599065590129726 18.17676676041005 23.757144153134355 24.467023496325865 33 31.295865706346053 19.358021890772985 25.26512788521522 24.08098451766574 34 27.93124964547053 22.241760735152305 28.31243972999036 21.514549889386807 35 26.669163460556334 23.140739731935128 29.305550826779246 20.884545980729293 36 28.44930349783727 24.966225037748487 23.92289090971016 22.661580554704088 37 29.75418677263696 23.596934430882772 24.797047970479706 21.85183082600057 38 30.82183222581928 22.399045834043278 25.095700573635487 21.68342136650196 39 29.95874578081849 21.495380209339594 24.374083713107023 24.171790296734894 40 28.722847719777096 20.457181849198225 26.77925622654384 24.040714204480835 41 26.2563985894665 21.510635877602095 25.976566943464906 26.2563985894665 42 25.90662372981646 20.43103742560963 26.143307427088903 27.519031417485007 43 25.565989414375963 18.64208070115531 29.746741790450177 26.045188094018553 44 25.843169840529068 18.789341286011858 28.053680580059876 27.313808293399198 45 26.052104208416832 20.59915285115686 27.82496811805429 25.523774822372015 46 25.372454325937397 20.0305253086701 26.63902683493553 27.95799353045697 47 24.9769228139352 20.550661546000523 28.637363388756565 25.835052251307705 48 27.72887926120169 20.811766047201 27.549880287310458 23.909474404286854 49 26.63817176725366 20.976805151877816 27.162477916452954 25.22254516441557 50 25.590227879285564 20.793129405324027 28.14845229133876 25.468190424051645 51 25.92525026794062 21.957448749230384 27.887487743141858 24.229813239687136 52 25.604790419161677 20.40604505275164 28.73567151411463 25.253493013972054 53 25.949836323611603 20.054064535261713 29.61116877487938 24.384930366247307 54 26.894087368154974 21.694969781924307 25.806006847898523 25.6049360020222 55 26.33056417746942 21.03569472338872 25.176876027022093 27.456865072119776 56 26.118108798831692 22.059830230010952 25.041073384446875 26.78098758671048 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 3.0 10 27.5 11 52.0 12 90.0 13 165.0 14 202.0 15 191.5 16 181.0 17 207.5 18 234.0 19 271.0 20 308.0 21 332.5 22 440.0 23 523.0 24 648.0 25 773.0 26 806.0 27 839.0 28 978.0 29 1152.0 30 1187.0 31 1440.5 32 1694.0 33 1342.5 34 991.0 35 1294.5 36 1598.0 37 1351.0 38 977.0 39 850.0 40 1027.0 41 1204.0 42 1162.5 43 1121.0 44 1430.0 45 1739.0 46 2458.5 47 2501.5 48 1825.0 49 2132.0 50 2439.0 51 2664.5 52 2890.0 53 3205.5 54 3789.5 55 4058.0 56 4348.0 57 4638.0 58 5901.0 59 7164.0 60 7590.0 61 8016.0 62 7779.0 63 6950.5 64 6359.0 65 5798.5 66 5238.0 67 4836.0 68 4434.0 69 3822.0 70 3210.0 71 2863.5 72 2193.5 73 1870.0 74 1681.5 75 1493.0 76 1132.5 77 772.0 78 604.5 79 361.5 80 286.0 81 228.5 82 171.0 83 130.0 84 89.0 85 67.0 86 45.0 87 27.0 88 7.5 89 6.0 90 4.0 91 2.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03279986427642368 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.006786178815811797 25 0.028275745065882485 26 0.07464796697392977 27 0.06333766894757677 28 0.10405474184244756 29 0.16286829157948313 30 0.23073007973760107 31 0.2691850930272013 32 0.2612678844087542 33 0.28275745065882485 34 0.30537804671153085 35 0.34157100039586047 36 0.3755018944749194 37 0.3845501328960018 38 0.4297913250014138 39 0.47955663631736695 40 0.5485494542781202 41 0.5711700503308262 42 0.6039699146072499 43 0.6333766894757678 44 0.6356387490810382 45 0.668438613357462 46 0.7001074478312503 47 0.7532658485551095 48 0.7973760108578861 49 0.7691002657920036 50 0.8335689645422157 51 0.8019001300684272 52 0.8369620539501216 53 0.8392241135553922 54 1.5619521574393485 55 0.8855963354634394 56 0.8674998586212747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 88415.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.51478821466946 #Duplication Level Percentage of deduplicated Percentage of total 1 67.72476171394224 26.76129616015382 2 12.805907776855483 10.12045467398066 3 6.14820963448493 7.288356048181869 4 3.5549703752468726 5.6189560594921675 5 2.375704840140825 4.693773680936493 6 1.5227409336806252 3.610247130011876 7 1.0848098004980393 3.000622066391449 8 0.8443770214958353 2.6692303342193067 9 0.589632767553024 2.096929254085845 >10 3.082691702206829 22.20211502573093 >50 0.1660131093110456 4.4562574223830795 >100 0.09731802959613017 6.337159984165583 >500 0.0 0.0 >1k 0.0028622949881214757 1.1446021602669232 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1012 1.1446021602669232 No Hit CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 318 0.3596674772380252 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 287 0.3246055533563309 No Hit CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 283 0.3200814341457897 No Hit CACAAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 279 0.3155573149352485 No Hit CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 259 0.2929367188825425 No Hit CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 240 0.2714471526324719 No Hit CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 236 0.2669230334219307 No Hit CACAAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 225 0.25448170559294236 No Hit CACAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 212 0.23977831815868347 No Hit CACAAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 196 0.22168184131651866 No Hit CACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 189 0.21376463269807158 No Hit CACAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 170 0.1922750664480009 No Hit CACAAGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT 162 0.1832268280269185 No Hit CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 160 0.1809647684216479 No Hit CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 156 0.17644064921110672 No Hit CACAAGGGGAGGTGATGTTTTCACTCTGGTTGTTTGCATCCTTCCCTGCTTGTGTT 147 0.16626138098738902 No Hit CACAAGGGGAGGTGATTGGTACCATCCTGTTTTCTCATCTCCCTCCCATTTCCTCT 144 0.16286829157948313 No Hit CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 133 0.15042696375049483 No Hit CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 133 0.15042696375049483 No Hit CACAAGGGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAA 128 0.14477181473731832 No Hit CACAAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 127 0.14364078493468302 No Hit CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 120 0.13572357631623594 No Hit CACAAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 119 0.13459254651360064 No Hit CACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 119 0.13459254651360064 No Hit CACAAGGGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGAGCACTGACTGTTCTTCC 115 0.13006842730305943 No Hit CACAAGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT 113 0.12780636769778883 No Hit CACAAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC 107 0.12102018888197703 No Hit CACAAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 106 0.11988915907934174 No Hit CACAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 106 0.11988915907934174 No Hit CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG 104 0.11762709947407113 No Hit CACAAGGGGAGTTAAGTTTTGTTTTTCTTCCCTATGATATCTTAGAAATAATTGTC 103 0.11649606967143583 No Hit CACAAGGGGAGGCAGAAGCTGCTATCTGATTGGTATAGCATCTGACTTTCCCTCTG 103 0.11649606967143583 No Hit CACAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 102 0.11536503986880053 No Hit CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 102 0.11536503986880053 No Hit CACAAGGGGCAGACTCCTAGTCACAGCTGGAGTAATGGGCACACAGAAAGCATCAT 95 0.10744783125035345 No Hit CACAAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA 95 0.10744783125035345 No Hit CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 92 0.10405474184244756 No Hit CACAAGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC 92 0.10405474184244756 No Hit CACAAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC 90 0.10179268223717695 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0022620596052705987 0.0 0.0 0.0 0.0 11 0.007917208618447096 0.0 0.0 0.0 0.0 12 0.009048238421082395 0.0 0.0 0.0 0.0 13 0.013572357631623594 0.0 0.0 0.0 0.0 14 0.016965447039529492 0.0 0.0 0.0 0.0 15 0.01809647684216479 0.0 0.0 0.0 0.0 16 0.01922750664480009 0.0 0.0 0.0 0.0 17 0.02035853644743539 0.0 0.0 0.0 0.0 18 0.02262059605270599 0.0 0.0 0.0 0.0 19 0.02262059605270599 0.0 0.0 0.0 0.0 20 0.02262059605270599 0.0 0.0 0.0 0.0 21 0.026013685460611887 0.0 0.0 0.0 0.0 22 0.030537804671153086 0.0 0.0 0.0 0.0 23 0.03279986427642368 0.0 0.0 0.0 0.0 24 0.03619295368432958 0.0 0.0 0.0 0.0 25 0.03619295368432958 0.0 0.0 0.0 0.0 26 0.03732398348696488 0.0 0.0 0.0 0.0 27 0.04071707289487078 0.0 0.0 0.0 0.0 28 0.04750325171068258 0.0 0.0 0.0 0.0 29 0.04976531131595317 0.0 0.0 0.0 0.0 30 0.052027370921223774 0.0 0.0 0.0 0.0 31 0.057682519934400274 0.0 0.0 0.0 0.0 32 0.05881354973703557 0.0 0.0 0.0 0.0 33 0.05994457953967087 0.0 0.0 0.0 0.0 34 0.06446869875021206 0.0 0.0 0.0 0.0 35 0.06673075835548267 0.0 0.0 0.0 0.0 36 0.07012384776338856 0.0 0.0 0.0 0.0 37 0.07464796697392977 0.0 0.0 0.0 0.0 38 0.07691002657920036 0.0 0.0 0.0 0.0 39 0.08256517559237686 0.0 0.0 0.0 0.0 40 0.08256517559237686 0.0 0.0 0.0 0.0 41 0.08482723519764745 0.0 0.0 0.0 0.0 42 0.08708929480291806 0.0 0.0 0.0 0.0 43 0.08935135440818866 0.0 0.0 0.0 0.0 44 0.09839959282927105 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTCAAG 15 0.008007487 50.616665 48 TTACAGG 15 0.008007487 50.616665 48 AGGGTTA 15 0.008284063 50.184044 44 TGAATGG 15 0.008284063 50.184044 46 CGCCAAA 15 0.008284063 50.184044 45 AAGAGTT 15 0.00830275 50.155464 42 TCACAAT 50 7.2759576E-12 50.155464 41 ATAATGA 15 0.00830275 50.155464 42 TGTGAAG 15 0.00830275 50.155464 40 ACTAGGG 15 0.00830275 50.155464 41 ACTAACC 25 2.0339166E-5 50.155464 41 TACTAGG 15 0.00830275 50.155464 40 TACTAAC 25 2.0339166E-5 50.155464 40 TCTATTT 15 0.00830275 50.155464 40 TCTGAGA 15 0.00832147 50.12692 39 GTTCTAT 15 0.00832147 50.12692 38 GTACTAG 15 0.00832147 50.12692 39 GCCTACT 25 2.0476766E-5 50.098408 37 CCGAGTA 15 0.008359001 50.069923 35 AGGATTT 20 4.1340844E-4 50.069923 35 >>END_MODULE