FastQCFastQC Report
Fri 17 Jun 2016
SRR1527002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565542
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT446917.9023308613683865No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT28870.5104837483334571No Hit
AGGACTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT26130.462034649946423No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT19130.3382595810744383No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT11410.2017533622613351No Hit
AGGACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG8100.14322543683758235No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAC7590.13420753896262347No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAG7540.1333234313278236No Hit
AGGACTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG6700.1184704230631854No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6440.11387306336222595No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAGCCATGCAT5920.1046783439603071No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAGCCATGCAT5890.10414787937942716No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGCTTAAGCCATGCAT5750.10167237800198749No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTACGCCATGCAT5750.10167237800198749No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAGCCATGCAT5720.10114191342110754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG453.092282E-1157.9663134
AGGGTAT150.00470503657.94061729
CGGTCGA150.004726535657.87393625
TACGCGA258.785561E-657.86369311
TTACGCG150.004729849357.86369312
TTACGTA150.00473150757.8585724
ATCGATT150.00473150757.8585724
GTATCGT202.0356674E-457.84833523
CTGGGTA120900.057.1049425
TGGGTAC116450.057.0673036
CATGCAT65500.056.87996758
GGTACCT115250.056.723998
GGGTACC115950.056.687157
ACTGGGT144350.056.6411974
GTACCTG115250.056.573539
TACCTGG115850.056.1807410
GTAGCAT102000.056.15127629
ACCTGGT115150.056.13004711
GTTGATC113350.056.0769916
CCTGGTT114850.056.07513412
Produced by FastQC (version 0.11.5)