FastQCFastQC Report
Fri 17 Jun 2016
SRR1527001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807031
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT12698915.735331108718253No Hit
AGGACTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT84511.0471716699854157No Hit
AGGACTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG59140.7328095203282153No Hit
AGGACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG28180.3491811343058693No Hit
AGGACTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG26470.32799235717091413No Hit
AGGACTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG19460.2411307620153377No Hit
AGGACTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT19210.23803298758040276No Hit
AGGACTGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC17870.22142891660915132No Hit
AGGACTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG16670.2065595993214635No Hit
AGGACTGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT16440.2037096468413233No Hit
AGGACTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC15990.1981336528584404No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC14920.18487517827691874No Hit
AGGACTGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC14600.18091002700020198No Hit
AGGACTGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT14560.1804143830906124No Hit
AGGACTGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT14200.17595358790430604No Hit
AGGACTGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGG12710.15749085227209364No Hit
AGGACTGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTC11970.14832143994468616No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC11350.14063895934604742No Hit
AGGACTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC10510.13023043724466596No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT10300.12762830671932057No Hit
AGGACTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGT10250.12700875183233357No Hit
AGGACTGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA10160.125893553035757No Hit
AGGACTGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT9860.12217622371383503No Hit
AGGACTGGGCCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT9740.12068929198506624No Hit
AGGACTGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA9460.1172197846179391No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC9230.11436983213779892No Hit
AGGACTGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGG9070.11238725649944054No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC9020.11176770161245354No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT8890.11015685890628736No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC8830.10941339304190298No Hit
AGGACTGGGCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA8360.10358957710422524No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGAG8270.10247437830764865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC303.6932033E-758.15125351
TTTAGCG150.004654674358.100645
ATATGCG202.00515E-458.028434
ATACGTT150.00473196657.8594139
TGGGTAC169350.056.691726
CTGGGTA174850.056.663145
CATGCAT147450.056.5857358
ACTGGGT211800.056.193564
GGTACCT168900.056.191578
GGGTACC169050.056.1417127
GTACCTG169050.056.0796559
ATTAAGC154100.055.7927551
TAGCATA159500.055.7303230
GTTGATC168750.055.6821916
GTAGCAT161250.055.65814229
TTGATCC168400.055.64330717
ACCTGGT170100.055.63143511
TACCTGG170100.055.6144310
CATATGC159750.055.59465433
CCTGGTT169700.055.5920912
Produced by FastQC (version 0.11.5)