FastQCFastQC Report
Fri 17 Jun 2016
SRR1527000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800106
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11785514.729923285164714No Hit
AGGACTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT78170.9769955480898781No Hit
AGGACTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG54100.6761604087458412No Hit
AGGACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG25550.31933268841878454No Hit
AGGACTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG25200.3149582680294861No Hit
AGGACTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG18220.2277198271229062No Hit
AGGACTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT16650.20809742709091045No Hit
AGGACTGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC16210.20259815574436385No Hit
AGGACTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC15280.1909746958527995No Hit
AGGACTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG14970.18710020922227805No Hit
AGGACTGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT14660.1832257225917566No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC14360.17947621940092937No Hit
AGGACTGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC14020.1752267824513252No Hit
AGGACTGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT13730.17160226270019222No Hit
AGGACTGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT13510.16885262702691892No Hit
AGGACTGGGTACCTGGTTGATCCTGNCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11990.14985514419339438No Hit
AGGACTGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGG11300.1412312868544918No Hit
AGGACTGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTC10720.13398224735222583No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC10530.13160756199803525No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT9940.12423353905607507No Hit
AGGACTGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT9750.1218588537018845No Hit
AGGACTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGT9430.11785938363166881No Hit
AGGACTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC9360.11698449955380912No Hit
AGGACTGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA8990.11236011228512222No Hit
AGGACTGGGCCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT8970.11211014540573373No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC8720.10898555941337773No Hit
AGGACTGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA8540.1067358574988814No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC8520.10648589061949292No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC8440.105486023101939No Hit
AGGACTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA8240.1029863543080542No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGAG8170.1021114702301945No Hit
AGGACTGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGG8050.10061166895386361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAC150.004529603858.50161445
TCGTACG150.00457637258.35003723
CTCGTAA150.00472938857.8673230
ATAGCCG150.004783596457.70097411
TGGGTAC162100.056.7787486
CTGGGTA167850.056.606965
CATGCAT141250.056.55709558
GGGTACC161350.056.5053757
GGTACCT160650.056.42468
GTACCTG161000.056.1957329
GCCAGTA154400.056.01758625
CCAGTAG154150.055.94867326
ACTGGGT202500.055.854114
TGCCAGT155000.055.8536924
ATCCTGC157100.055.80416520
TAGCATA152800.055.6707930
ACCTGGT161500.055.62874211
GTAGCAT154500.055.6013229
ATTAAGC147250.055.5853451
TTGATCC160100.055.58517
Produced by FastQC (version 0.11.5)