FastQCFastQC Report
Fri 17 Jun 2016
SRR1526999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739480
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG13283817.963704224590252No Hit
AGGACTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC69150.9351165683994158No Hit
AGGACTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC47280.6393682046843728No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC27380.3702601828311787No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA26480.35808946827500404Illumina Single End Adapter 1 (95% over 21bp)
AGGACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC22690.3068372369773354No Hit
AGGACTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT22650.30629631633039434No Hit
AGGACTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC16990.22975604478822956No Hit
AGGACTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA15920.21528641748255528No Hit
AGGACTGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT15350.20757829826364474No Hit
AGGACTGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC14770.19973494888299884No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13600.18391301995997186No Hit
AGGACTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC13150.17782766268188455No Hit
AGGACTGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG12490.1689024720073565No Hit
AGGACTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG12490.1689024720073565No Hit
AGGACTGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA12150.16430464650835722No Hit
AGGACTGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAA11350.15348623356953536No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT10370.14023367771947853No Hit
AGGACTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG10070.13617677286742036No Hit
AGGACTGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT9990.13509493157353816No Hit
AGGACTGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT9710.1313084870449505No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC8790.11886731216530534No Hit
AGGACTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA8700.11765024070968788No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC8550.1156217882836588No Hit
AGGACTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC8420.11386379618110022No Hit
AGGACTGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT8250.11156488343160059No Hit
AGGACTGGGCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA8220.11115919294639477No Hit
AGGACTGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG7960.10764320874127764No Hit
AGGACTGGGCCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA7830.1058852166387191No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTTGTCTCAAAGATTAAGC7580.10250446259533726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA150.00830208850.1934943
ACGTCAC150.00830432350.19006742
ATAAGTA150.0084234750.00950230
CATATCG301.0657368E-649.87408423
GATTAAG153650.049.3861650
AAGATTA155150.049.03871548
AGATTAA154700.048.9821349
TGGGTAC176000.048.8959166
CTGGGTA181850.048.8300675
GTACCTG173150.048.637159
GGTACCT173700.048.596678
AAAGATT157100.048.56517447
GGGTACC174400.048.530197
ATATGCT160050.048.4760134
GTCTCAA157250.048.46652642
GCATATG161150.048.45549832
TAGCATA160600.048.43697730
CATATGC161150.048.42866533
CTTGTCT158000.048.40337839
TATGCTT160450.048.39624435
Produced by FastQC (version 0.11.5)