FastQCFastQC Report
Fri 17 Jun 2016
SRR1526998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164080
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT60783.704290589956119No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.5369332033154559No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.27547537786445636No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT4260.25962944904924423No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.21452949780594832No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT3350.20416869819600195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT258.473104E-658.187127
GGGTACG201.973562E-458.1871157
AAAGTAG406.9303496E-1058.1871152
TGGGCGA406.9303496E-1058.1871156
TACCAAA258.581583E-658.06244746
TCGGGTA201.9946099E-458.06244751
ACGATTA550.058.06244748
AACGATT453.092282E-1158.06244747
TTACGCC258.581583E-658.06244752
ACTAAGC150.004660156658.0624451
GATTCAG150.004660156658.0624450
TCAAGTT150.004660156658.0624448
TACGCCA258.597175E-658.04467853
GTCGGAC258.597175E-658.04467855
GAACCCT150.004671465658.02691744
AACGTAC150.00467712858.00917437
ATAGAAT150.00467712858.00917439
AATTGAG150.00467712858.00917440
ACCAGTA258.628425E-658.00917441
GTCAAAT150.00467712858.00917438
Produced by FastQC (version 0.11.5)