FastQCFastQC Report
Fri 17 Jun 2016
SRR1526997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230319
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT186538.098767361789518No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13350.5796308598074844No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC7530.32693785575658113No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7480.32476695365992386No Hit
CAAAGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC5740.2492195606962517No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5110.22186619427837043No Hit
CAAAGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4620.2005913537311294No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4300.18669758031252306No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4000.1736721677325796No Hit
CAAAGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT3910.16976454395859655No Hit
CAAAGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG3500.15196314676600714No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3460.15022642508868136No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3320.14414789921804108No Hit
CAAAGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2710.11766289363882268No Hit
CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2700.11722871321949123No Hit
CAAAGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2540.11028182651018806No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2440.10594002231687355No Hit
CAAAGTGGGGTTCTTGATCAGTCTCAGATGCCAGGAATTATTTTTTGTCGCCCGGGAGGGGGAG2340.10159821812355907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGT150.00460005158.26066253
TCGTCAG150.00460005158.26066254
GAATTCG150.00460005158.26066254
AATAGAC150.00460005158.26066253
TCGTGAT201.9629592E-458.2606656
CGACAGA150.00462799958.1716249
CGCTAGT150.00463200258.1589247
TACTATC150.004648039658.1081843
GTACGAT258.552595E-658.1081845
TTAGATA201.988563E-458.10817744
GTTAGAT201.988563E-458.10817743
ACTAATT150.004652055458.0955139
CGTACAC351.6125341E-858.0955139
TACTCAT150.004652055458.0955139
CGTCGAT201.9907086E-458.09550540
AATCCGT150.004660094658.07018736
GCGACAT201.9950053E-458.07018337
CATGCGT201.9950053E-458.07018336
ATATCCC201.9950053E-458.07018336
ACGTACA351.617991E-858.07018338
Produced by FastQC (version 0.11.5)