FastQCFastQC Report
Fri 17 Jun 2016
SRR1526996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229583
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT172527.514493668956325No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.585409198416259No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC7300.3179677937826407No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6190.26961926623486926No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.20733242443909172No Hit
CAAAGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC4660.20297670123658984No Hit
CAAAGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4300.18729609770758288No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3980.1733577834595767No Hit
CAAAGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT3600.1568060352900694No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3590.1563704629698192No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3330.14504558264331419No Hit
CAAAGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG3180.1385119978395613No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2760.1202179603890532No Hit
CAAAGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2680.11673338182705165No Hit
CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2320.10105277829804472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCG150.00454673558.43232342
TACATCT150.00455865658.393740
CGTACAC351.5485057E-858.393739
TACGGTT150.00456661658.3679824
GCCCGAC452.910383E-1158.3679825
GTACGGT150.00456661658.3679823
CTAATTC150.004666992558.04840558
GTTATAA150.004666992558.04840558
CCATTCA150.004666992558.04840558
TACGTAA351.6227204E-858.048458
CGCTAAT150.004671041458.03569456
CTAATCG150.00467509358.02298749
TAATCGC150.00467509358.02298750
AACGTAC351.645276E-857.94687737
TAACGTA351.645276E-857.94687736
ACGTACA351.645276E-857.94687738
CATACAG150.004699457457.94687337
CGGTCAA150.004699457457.94687336
GTCATAC150.004703527357.93420835
TTGCGAT150.004703527357.93420835
Produced by FastQC (version 0.11.5)