FastQCFastQC Report
Fri 17 Jun 2016
SRR1526995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205040
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG193169.420600858369099No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14900.7266874756145143No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6930.33798283261802575No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6620.32286383144752245No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC6070.2960397971127585No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC5020.24483027701911825No Hit
CAAAGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4220.20581349980491612No Hit
CAAAGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG4090.1994732735076083No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3990.194596176355833No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3910.1906944986344128No Hit
CAAAGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA3820.18630511119781507No Hit
CAAAGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2940.14338665626219274No Hit
CAAAGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC2890.1409481076863051No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA2700.1316816230979321Illumina Single End Adapter 1 (95% over 21bp)
CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG2420.11802575107296137No Hit
CAAAGTGGGGTTCTTGATCAGTCTCAGATGCCAGGAATTATTTTTTGTCGCCCGGG2300.11217323449083105No Hit
CAAAGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2200.1072961373390558No Hit
CAAAGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2190.10680842762387827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGT150.00798199850.6818349
CATATTA203.9056747E-450.68182850
CGCGAAC203.9056747E-450.68182849
AGTCCGC150.00822798150.29427745
ACGTTAA150.00822798150.29427747
TATATAA150.00822798150.29427746
TTTGTCG204.056516E-450.29427344
ATTATAC150.00824404250.26947842
CACGAGT150.00827623450.21994839
ATGCTCG150.00830043950.18287336
CGGAATA204.1061494E-450.1705235
GTAAATT150.0083085250.1705235
AGTAAAT150.00834900850.1088734
TATGCGT204.1411788E-450.08425532
ATGCGTT204.1411788E-450.08425533
CCTATGC150.00836524550.08425533
CACAAAT150.00836524550.08425532
GTATGCG204.1462024E-450.07195331
GAGCATA204.1512307E-450.05965830
TACACAA204.1512307E-450.05965830
Produced by FastQC (version 0.11.5)