##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526995_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 205040 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.02457569254779 38.0 38.0 40.0 35.0 40.0 2 37.51359734685915 38.0 38.0 40.0 33.0 40.0 3 37.70448205228248 38.0 38.0 40.0 35.0 40.0 4 37.61193913382755 38.0 38.0 40.0 34.0 40.0 5 37.495313109637145 38.0 38.0 40.0 34.0 40.0 6 36.56404116269996 38.0 36.0 39.0 32.0 40.0 7 38.423088177916505 39.0 38.0 40.0 36.0 40.0 8 38.34661041747952 39.0 38.0 40.0 36.0 40.0 9 38.34209422551697 39.0 38.0 40.0 36.0 40.0 10 37.35018532969177 39.0 37.0 40.0 32.0 40.0 11 37.29964397190792 39.0 36.0 40.0 33.0 40.0 12 37.285002926258294 39.0 36.0 40.0 33.0 40.0 13 37.27999902458057 39.0 37.0 40.0 33.0 40.0 14 37.48509559110418 39.0 37.0 40.0 33.0 40.0 15 37.423395435037065 39.0 37.0 40.0 33.0 40.0 16 37.70051697229809 39.0 38.0 40.0 34.0 40.0 17 37.52448790479907 39.0 38.0 40.0 33.0 40.0 18 37.57072278579789 39.0 38.0 40.0 33.0 40.0 19 37.22575595005853 39.0 36.0 40.0 33.0 40.0 20 37.22425868123293 39.0 37.0 40.0 33.0 40.0 21 37.19278189621537 39.0 37.0 40.0 33.0 40.0 22 37.11723566133438 39.0 36.0 40.0 33.0 40.0 23 37.12730198985564 39.0 37.0 40.0 33.0 40.0 24 36.83140362856028 39.0 36.0 40.0 32.0 40.0 25 36.562222005462345 38.0 36.0 40.0 31.0 40.0 26 36.28775360905189 38.0 36.0 40.0 31.0 40.0 27 36.2078374951229 38.0 36.0 40.0 31.0 40.0 28 35.595449668357396 38.0 35.0 39.0 29.0 40.0 29 35.7210056574327 38.0 35.0 39.0 30.0 40.0 30 35.20091201716738 38.0 35.0 39.0 29.0 40.0 31 34.759334763948495 38.0 34.0 39.0 27.0 40.0 32 34.53858759266485 38.0 33.0 39.0 27.0 40.0 33 34.304716152945765 38.0 33.0 39.0 26.0 40.0 34 34.49720054623488 38.0 34.0 39.0 26.0 40.0 35 34.170922746781116 38.0 33.0 39.0 25.0 40.0 36 34.00596468981662 38.0 33.0 39.0 23.0 40.0 37 33.666143191572374 38.0 33.0 39.0 23.0 40.0 38 33.66912797502926 38.0 33.0 39.0 22.0 40.0 39 33.48502731174405 38.0 33.0 39.0 20.0 40.0 40 33.11660651580179 37.0 33.0 39.0 18.0 40.0 41 32.797029847834565 37.0 32.0 39.0 11.0 40.0 42 32.49619586422161 37.0 32.0 39.0 10.0 40.0 43 32.18680257510729 36.0 32.0 39.0 4.0 40.0 44 32.18784139680062 36.0 32.0 39.0 4.0 40.0 45 31.74961470932501 36.0 31.0 39.0 4.0 40.0 46 31.35654506437768 36.0 31.0 39.0 4.0 40.0 47 30.8200546234881 36.0 30.0 39.0 4.0 40.0 48 30.82497073741709 36.0 30.0 39.0 4.0 40.0 49 30.50607198595396 35.0 30.0 39.0 4.0 40.0 50 30.07480979321108 35.0 29.0 39.0 4.0 40.0 51 29.154716152945767 35.0 28.0 38.0 4.0 40.0 52 28.941167577058135 35.0 27.0 38.0 4.0 40.0 53 28.778014046039797 35.0 27.0 38.0 4.0 40.0 54 28.18876804525946 34.0 24.0 38.0 4.0 40.0 55 27.89975126804526 34.0 23.0 38.0 4.0 40.0 56 16.366148068669528 20.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.8520944677863582 1 2 -1.392435155760431 1 3 -1.052265835983036 1 4 -1.4852537330831836 1 5 -1.949006634140929 1 6 -1.054070593366852 1 7 -1.2593266259486455 1 8 -1.2369682883488196 1 9 -1.4029276000753086 1 10 -1.5632130036420833 1 11 -1.6368257281618455 1 12 -1.5200015647021132 1 13 -0.8521568590692254 1 14 -1.5735214347922195 1 15 -1.6172681948925174 1 16 -1.175268558581955 1 17 -0.7332201692960467 1 18 -1.2362087491943399 1 19 -0.7468326529420821 1 20 -0.9924058372882811 1 21 -0.748123128724373 1 22 -1.2172394559813497 1 23 -0.5478652318436588 1 24 -0.5007705510255178 1 25 -1.223834571050162 1 26 -0.23376095207729009 1 27 -1.2928378934426163 1 28 -0.0073118948462749245 1 29 -0.7606541033404781 1 30 -0.6522100556648596 1 31 -0.9252088517489696 1 32 -0.5784764385538423 1 33 -1.2311722974683335 1 34 -0.6081439739566719 1 35 -1.386690576713356 1 36 0.04059380124398615 1 37 0.18749904486126212 1 38 0.23881716367480266 1 39 0.058918001955795773 1 40 0.44924557080257443 1 41 0.49046253475834334 1 42 0.07487319519312052 1 43 0.17383124877975575 1 44 0.6620838354843386 1 45 0.6448539397092752 1 46 1.3272286332206207 1 47 1.2095052991012025 1 48 0.8513367070589268 1 49 1.673300268679224 1 50 1.385160643722294 1 51 1.4018609134828566 1 52 1.717992290426789 1 53 1.4810894465136357 1 54 1.8391497857632118 1 55 1.6774164464947567 1 56 1.9819880993461112 2 1 -0.0654486263181866 2 2 0.0912300791738403 2 3 -0.024880699968463205 2 4 -0.08369184319641931 2 5 -0.1279992151639675 2 6 -0.3817811231078352 2 7 -0.21150676391557965 2 8 -0.09223315884212013 2 9 -0.09088803219817976 2 10 -0.05221469568945736 2 11 -0.4297451606751892 2 12 -0.1017274530269816 2 13 0.10135088530146419 2 14 -0.21517183177256527 2 15 -0.14201113384865494 2 16 0.009945550490023436 2 17 -0.19642464671372295 2 18 -0.08887046987636893 2 19 0.08924700344214642 2 20 0.05635115867683993 2 21 0.11830925445928386 2 22 0.04383043859107971 2 23 0.18140718778409592 2 24 0.24149158616047828 2 25 -0.2918288441205732 2 26 0.1522080704399542 2 27 0.3889530736275333 2 28 0.020073241168297784 2 29 0.30119673617533493 2 30 0.046397259186115036 2 31 0.09954710295622249 2 32 0.22712033354039107 2 33 -0.06571609546391244 2 34 0.21723673670322086 2 35 0.04759290630109092 2 36 -0.06113729338051144 2 37 0.5147149761381087 2 38 0.46922195347045204 2 39 0.7522409226898858 2 40 -0.2217943875470283 2 41 1.247692783359156 2 42 0.8039894258321922 2 43 0.8237336306618346 2 44 1.1019120275967964 2 45 0.9722000284766814 2 46 1.344305295982565 2 47 0.9684796580755624 2 48 1.3349238474988603 2 49 1.5366088720893565 2 50 1.2921501009655572 2 51 1.6587657683572559 2 52 1.966150561935315 2 53 1.6945216995397985 2 54 1.6911086559961923 2 55 2.2534154052348327 2 56 1.5354437807205734 3 1 -0.5326510756101825 3 2 -0.5597458106080211 3 3 -0.6277892342202804 3 4 -0.6474882457987903 3 5 -0.6663842132504598 3 6 -0.9853785207350896 3 7 -0.24763382093816233 3 8 -0.12476281823744984 3 9 -0.10787999545880211 3 10 -0.3656475315103549 3 11 -0.700315386469299 3 12 -0.34650357242996677 3 13 0.0539723931468572 3 14 -0.28375200781542986 3 15 -0.16129930836071082 3 16 0.027549836751028067 3 17 -0.7051119792816536 3 18 -0.37283526130384814 3 19 -0.23972352851346557 3 20 -0.3059144364245796 3 21 -0.22528048913046206 3 22 0.2115304003208962 3 23 0.20628280967464008 3 24 -0.20963738437147583 3 25 0.20660207819094012 3 26 0.31087626791411793 3 27 0.7424930659734983 3 28 0.5912940601503109 3 29 0.48749600904177015 3 30 1.0420344578083842 3 31 0.7730258630021467 3 32 0.8086741031538622 3 33 0.7463007434186011 3 34 0.1933561396882979 3 35 0.4771374910695556 3 36 -0.08540059227067331 3 37 0.3436472379061897 3 38 0.0056934421807497415 3 39 -0.16195731688149095 3 40 0.024895241232158583 3 41 0.16548842055777158 3 42 0.20245861833123513 3 43 0.01730423915780932 3 44 0.15196943287808296 3 45 0.4126898868769864 3 46 0.43339829253824647 3 47 0.5129878861658774 3 48 0.14188902162821293 3 49 0.47583581430136945 3 50 0.7596587117577513 3 51 0.5750305980549193 3 52 0.41559564421621786 3 53 0.5147283585524249 3 54 0.7532594405350359 3 55 0.7986124967005814 3 56 0.1817507076245164 4 1 -0.672512990525405 4 2 -0.7028567863415986 4 3 -0.7784390463839514 4 4 -1.122231874593595 4 5 -1.0227401884763694 4 6 -1.0767837395390245 4 7 -0.411559092539342 4 8 -0.4608883132115622 4 9 -0.3318875089119402 4 10 -1.0845276208595251 4 11 -1.0159565683151683 4 12 -1.0670335754759606 4 13 -1.058957853578704 4 14 -0.726893443494177 4 15 -0.7456479731997803 4 16 -0.718338070650745 4 17 -0.616701988419635 4 18 -0.984344043921368 4 19 -0.6982726197917657 4 20 -0.6492741683770618 4 21 -0.41915804015086877 4 22 -0.5899215237323077 4 23 -0.3458036965696465 4 24 -0.1978595389049289 4 25 -0.670923558929573 4 26 -0.4422712597536602 4 27 -1.0378653460480294 4 28 -0.23348510524719046 4 29 -0.2451925887854216 4 30 -0.2919334577160342 4 31 -0.1399348436685841 4 32 0.4880831802175294 4 33 0.17256229281447588 4 34 0.014175512213427055 4 35 -0.02014649715259509 4 36 -0.42327753409217905 4 37 -0.3078909893250028 4 38 -0.2562620862993015 4 39 -0.198230034923931 4 40 0.39604967315418094 4 41 0.4666357451592589 4 42 0.23093866706715005 4 43 0.5991653418078258 4 44 0.9727126555402776 4 45 0.5959572236624489 4 46 0.44286625882924113 4 47 0.9390971358358975 4 48 0.4209782692675681 4 49 0.3170379668041612 4 50 1.8924640727081012 4 51 0.10363756322905004 4 52 -0.5029493857360627 4 53 0.1255264091159347 4 54 -0.5176563884831396 4 55 -0.18146162249933795 4 56 0.2547530428869784 5 1 -0.39027019650058037 5 2 -0.43639349999612875 5 3 -0.44912072111569756 5 4 -0.457125625908553 5 5 -0.62609596377618 5 6 -0.8703351807340596 5 7 -0.31867039066683844 5 8 -0.1321485698915481 5 9 -0.09482670520901593 5 10 0.14022516685349729 5 11 -0.1931604395543829 5 12 -0.5955418860166759 5 13 -0.20575987335568158 5 14 -0.3064221621981531 5 15 -0.1816357703805096 5 16 0.1477991058693533 5 17 0.04261844078297372 5 18 -0.3491136631092502 5 19 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0.6746684378532066 6 45 0.8796519515536012 6 46 1.4955873472646175 6 47 1.9595052991012025 6 48 1.8005488474988631 6 49 1.183003102858585 6 50 1.599521895837352 6 51 1.82238756322905 6 52 2.366310818345571 6 53 1.7651947764628737 6 54 2.038784938047474 6 55 2.1841846360040655 6 56 1.530475832002626 7 1 0.022852655733096583 7 2 0.017912771481533696 7 3 0.04851673592897754 7 4 0.0760036696240931 7 5 0.19027001560526458 7 6 0.30395605637934153 7 7 -0.16134650750532842 7 8 -0.12468508191903993 7 9 -0.040327134762279115 7 10 0.29153530431054264 7 11 -0.1657227247777513 7 12 -0.19756078636032015 7 13 -0.03270360187801913 7 14 -0.39802439587512595 7 15 0.029543353330829802 7 16 -0.38989419309972106 7 17 -0.22182528773936383 7 18 0.03372568396978437 7 19 -0.21772415040400972 7 20 0.045453722779399186 7 21 -0.13153048913046206 7 22 -0.1298875101268706 7 23 -0.052967812215904075 7 24 0.003671073339965858 7 25 -0.021476280018013938 7 26 0.09780101915789885 7 27 -0.03308218278272079 7 28 0.2601373437324028 7 29 0.13076404386764295 7 30 0.21274341303226407 7 31 0.2756688978280195 7 32 0.14146328225834282 7 33 0.3816396737668555 7 34 0.6880700700365594 7 35 0.739419829378015 7 36 0.912500527132309 7 37 1.4193624120355395 7 38 0.8993501585986579 7 39 1.053683230382191 7 40 1.2766831765555366 7 41 1.3792632961796656 7 42 1.7881240412168111 7 43 1.2198073486105514 7 44 1.1277934378532066 7 45 0.6504852848869369 7 46 0.4747540139312818 7 47 0.4751302991012025 7 48 0.6703405141655274 7 49 0.7715447695252493 7 50 0.2974385625040199 7 51 0.8171792298957179 7 52 0.9444358183455712 7 53 0.8016531097962059 7 54 0.6481599380474741 7 55 1.1373096360040655 7 56 0.7075591653359581 8 1 -0.7149288757984351 8 2 -1.4633090753653164 8 3 -1.0250193001070613 8 4 -0.9024028619074329 8 5 -0.8107434979082484 8 6 -0.7049966463233588 8 7 -0.6055469579557737 8 8 -0.7108055774145399 8 9 -0.8943530356631797 8 10 -0.5909534344282008 8 11 -0.3163702473002772 8 12 -0.5557815070810364 8 13 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3.216403181290719 8 55 3.3688693206887486 8 56 3.4119510572278493 9 1 0.012435989066432285 9 2 0.184579438148198 9 3 -0.040024930737693865 9 4 0.1541286696240931 9 5 0.20589501560526458 9 6 0.28833105637934153 9 7 -0.015513174171992716 9 8 0.01593991808096007 9 9 0.06383953190438518 9 10 0.07278530431054264 9 11 0.5686522752222487 9 12 0.6618142136396798 9 13 0.6339630647886452 9 14 0.5030172707915384 9 15 0.5660016866641655 9 16 0.47989747356694323 9 17 0.29379971226063617 9 18 0.5493506839697844 9 19 0.6729008495959903 9 20 0.7954537227793992 9 21 0.41534451086953794 9 22 0.6774041565398008 9 23 0.5876571877840959 9 24 0.34742107333996586 9 25 0.5462320533153218 9 26 0.6238426858245703 9 27 0.1960844838839435 9 28 0.6351373437324028 9 29 0.19326404386764295 9 30 0.4679517463655998 9 31 0.7183772311613481 9 32 1.1622966155916785 9 33 0.8555980071001912 9 34 1.3599450700365594 9 35 1.239419829378015 9 36 1.0739588604656447 9 37 1.7266540787022109 9 38 1.2066418252653222 9 39 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-0.2963651354601424 120 38 -0.35259284658268797 120 39 -0.08928956754527206 120 40 -0.3367937232717466 120 41 -0.2902639058928713 120 42 -0.08908882579527955 120 43 -0.0051224873134572135 120 44 0.011995726281526231 120 45 0.4462268651978185 120 46 0.32028510201418214 120 47 0.5825622939198567 120 48 0.40366305302563177 120 49 0.6509705035494306 120 50 0.89669374385117 120 51 0.972808547685009 120 52 1.1002810255994575 120 53 1.0315484034058784 120 54 0.6073568292391833 120 55 0.7843573475757388 120 56 0.19363429486963568 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 202.0 5 22.0 6 33.0 7 52.0 8 137.0 9 131.0 10 135.0 11 169.0 12 265.0 13 327.0 14 439.0 15 626.0 16 747.0 17 946.0 18 1177.0 19 1383.0 20 1487.0 21 1956.0 22 1899.0 23 2019.0 24 2731.0 25 3071.0 26 2953.0 27 3264.0 28 3802.0 29 4214.0 30 5051.0 31 5914.0 32 7255.0 33 9093.0 34 11833.0 35 16262.0 36 23336.0 37 34088.0 38 36710.0 39 21311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.72493172063987 0.20044869293796333 0.04974639094810769 0.024873195474053846 8 99.58770834146532 0.290799797025645 0.07026035364378001 0.05123150786525626 9 98.43689036285603 0.8671478735856419 0.2936012485368708 0.4023605150214592 10 46.35827155676941 26.944986344127976 17.53511509949278 9.161626999609833 11 27.202984783456884 37.44147483417869 17.39953179867343 17.956008583690988 12 29.259168942645335 15.868123293015998 23.352516582130317 31.52019118220835 13 22.06203667577058 15.097054233320328 25.805208739758097 37.03570035115099 14 22.475614514241123 18.72805306281701 38.244732735076084 20.55159968786578 15 35.00341396800624 17.55950058525166 25.371634802965275 22.065450643776824 16 36.07003511509949 18.60417479516192 23.100370659383536 22.225419430355053 17 27.620952009364025 18.716348029652753 30.819352321498243 22.84334763948498 18 25.904213811939137 19.674697619976588 33.1379243074522 21.283164260632073 19 36.026141240733516 19.7634607881389 22.05423332032774 22.156164650799845 20 23.42965835101324 33.411368790694276 19.751749701270516 23.407223157021974 21 23.54906359734686 19.204545454545453 34.446937182988684 22.799453765119 22 23.76999609832228 19.64299648849005 21.395337495122906 35.19166991806477 23 25.188255950058526 22.343932891143194 20.286773312524385 32.181037846273895 24 24.153639800024386 22.888915985855384 31.100597488111205 21.856846726009024 25 35.504329796316625 20.603488230271985 19.944871325771434 23.947310647639956 26 25.66483975031601 19.156755279430353 20.309518348064167 34.86888662218947 27 22.993279615810717 18.266072552818706 21.432998696918972 37.30764913445161 28 23.853300590254214 32.96001015491122 21.172501672142833 22.014187582691736 29 37.964053133747946 19.529925595165395 21.731292533623854 20.774728737462812 30 24.51139069674195 20.506676967761813 34.001437589543734 20.9804947459525 31 25.425271160817815 31.51336356831491 22.03435437551064 21.027010895356632 32 37.37892100268086 19.78161751756257 23.09257773516232 19.74688374459425 33 25.608377578233956 20.6381052580069 22.091947835873846 31.661569327885296 34 23.244806860230632 33.13477690545647 24.78708616402686 18.83333007028604 35 22.107964116425745 24.516688212480904 36.051533670231514 17.32381400086183 36 23.6673491981072 34.48334982512173 23.16377815442192 18.68552282234915 37 23.26643185013545 22.97740176651268 34.97900857782502 18.77715780552684 38 35.08037639678495 23.138110174475592 23.164575573416975 18.616937855322487 39 24.1577964198687 21.215538417034796 22.86685069057997 31.759814472516535 40 22.877301787457867 22.359660270154897 34.7874725846258 19.975565357761432 41 21.8648933820823 22.05923490295193 35.48646725394449 20.589404461021275 42 32.25934706739902 21.25295125339302 25.317704575240395 21.169997103967564 43 20.58218387700285 20.697579635349403 37.08525045790019 21.63498602974755 44 21.168048081077895 20.53118984955022 25.89317280119417 32.40758926817771 45 21.5849141869124 20.75772907231484 36.14612293817724 21.511233802595513 46 21.56167076167076 21.574938574938574 25.156265356265354 31.707125307125306 47 20.85891681701316 32.55054837552048 26.498508005643522 20.092026801822836 48 21.762888930422893 32.03862006551443 25.20141259332855 20.997078410734137 49 22.092851734209205 33.747996341787214 23.985386816666505 20.173765107337076 50 31.280719103777066 22.549728663291564 25.510570569684088 20.658981663247282 51 20.92288926705948 33.63106070293826 25.26731524016565 20.17873478983661 52 21.83236826440101 22.5452237697216 35.6147333310375 20.00767463483989 53 22.092113614335126 22.308596844264915 35.52834208286388 20.070947458536082 54 22.122941593569788 32.59137316348743 24.52484963282145 20.760835610121333 55 21.236807907379696 33.242104434118296 24.15728124753879 21.36380641096322 56 31.745859888284656 22.311572627279215 23.333743448411624 22.608824036024508 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.5 4 5.5 5 8.0 6 21.0 7 34.0 8 290.0 9 546.0 10 447.5 11 349.0 12 293.5 13 233.0 14 228.0 15 379.0 16 530.0 17 674.5 18 819.0 19 1018.0 20 1217.0 21 1459.0 22 2013.5 23 2326.0 24 2688.5 25 3051.0 26 3256.0 27 3461.0 28 3766.5 29 4446.0 30 4820.0 31 4109.0 32 3398.0 33 3893.5 34 4389.0 35 3612.0 36 2835.0 37 2932.0 38 2967.0 39 2905.0 40 3393.5 41 3882.0 42 4613.5 43 5345.0 44 15593.0 45 25841.0 46 16419.0 47 6896.5 48 6796.0 49 7099.5 50 7403.0 51 8129.0 52 8855.0 53 9031.5 54 9322.5 55 9437.0 56 10762.0 57 12087.0 58 13163.0 59 14239.0 60 13641.0 61 13043.0 62 12138.5 63 9471.0 64 7708.0 65 7399.5 66 7091.0 67 6305.5 68 5520.0 69 4556.0 70 3592.0 71 3025.5 72 2119.0 73 1779.0 74 1401.5 75 1024.0 76 831.5 77 639.0 78 539.0 79 332.5 80 226.0 81 179.0 82 132.0 83 102.0 84 72.0 85 45.5 86 19.0 87 13.5 88 6.0 89 4.0 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.04291845493562232 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.002438548575887632 21 0.0 22 0.0 23 0.0 24 0.007315645727662894 25 0.03170113148653921 26 0.06876706984003121 27 0.06876706984003121 28 0.1038821693328131 29 0.17021069059695668 30 0.2589738587592665 31 0.3126219274287944 32 0.30628170113148656 33 0.3340811548966055 34 0.3570035115099493 35 0.4018728053062817 36 0.43796332422941864 37 0.4433281310963715 38 0.48770971517752637 39 0.527214202106906 40 0.600370659383535 41 0.6218298868513461 42 0.6403628560280921 43 0.6793796332422942 44 0.6749902458056964 45 0.7110807647288334 46 0.7510729613733905 47 0.7905774483027702 48 0.8417869683964104 49 0.8105735466250488 50 0.8715372610222396 51 0.8398361295357003 52 0.8647093250097542 53 0.8734880998829497 54 1.642118611002731 55 0.9207959422551697 56 0.899824424502536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 205040.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.574326960593055 #Duplication Level Percentage of deduplicated Percentage of total 1 68.92068487305313 25.20727662895045 2 12.714689566887136 9.300624268435428 3 5.947301045444847 6.525555989075301 4 3.391028376360145 4.960983222785798 5 2.192233838276083 4.0089738587592665 6 1.4241519095370172 3.1252438548575885 7 1.0147749093236613 2.5980296527506828 8 0.7267441860465116 2.126414358174015 9 0.5240558992959249 1.7250292625829107 >10 2.8549711969276723 18.888997268825594 >50 0.19735438446767656 4.984880998829497 >100 0.0840089609558353 5.198985563792431 >500 0.005333902282910178 1.201716738197425 >1k 0.0013334755707275444 0.7266874756145143 >5k 0.0 0.0 >10k+ 0.0013334755707275444 9.420600858369099 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 19316 9.420600858369099 No Hit CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1490 0.7266874756145143 No Hit CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 693 0.33798283261802575 No Hit CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 662 0.32286383144752245 No Hit CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 607 0.2960397971127585 No Hit CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 502 0.24483027701911825 No Hit CAAAGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 422 0.20581349980491612 No Hit CAAAGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG 409 0.1994732735076083 No Hit CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 399 0.194596176355833 No Hit CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 391 0.1906944986344128 No Hit CAAAGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 382 0.18630511119781507 No Hit CAAAGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 294 0.14338665626219274 No Hit CAAAGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 289 0.1409481076863051 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 270 0.1316816230979321 Illumina Single End Adapter 1 (95% over 21bp) CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 242 0.11802575107296137 No Hit CAAAGTGGGGTTCTTGATCAGTCTCAGATGCCAGGAATTATTTTTTGTCGCCCGGG 230 0.11217323449083105 No Hit CAAAGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 220 0.1072961373390558 No Hit CAAAGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 219 0.10680842762387827 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.754194303550526E-4 0.0 0.0 0.0 0.0 8 0.005852516582130316 0.0 0.0 0.0 0.0 9 0.006340226297307842 0.0 0.0 0.0 0.0 10 0.016582130316035896 0.0 0.0 0.0 0.0 11 0.029750292625829105 0.0 0.0 0.0 0.0 12 0.032188841201716736 0.0 0.0 0.0 0.0 13 0.035115099492781895 0.0 0.0 0.0 0.0 14 0.03852906749902458 0.0 0.0 0.0 0.0 15 0.03901677721420211 0.0 0.0 0.0 0.0 16 0.04194303550526726 0.0 0.0 0.0 0.0 17 0.04535700351150995 0.0 0.0 0.0 0.0 18 0.048283261802575105 0.0 0.0 0.0 0.0 19 0.05072181037846274 0.0 0.0 0.0 0.0 20 0.059012875536480686 0.0 0.0 0.0 0.0 21 0.06145142411236832 0.0 0.0 4.877097151775263E-4 0.0 22 0.06632852126414358 0.0 0.0 4.877097151775263E-4 0.0 23 0.07657042528287164 0.0 0.0 4.877097151775263E-4 0.0 24 0.083398361295357 0.0 0.0 4.877097151775263E-4 0.0 25 0.08583690987124463 0.0 0.0 4.877097151775263E-4 0.0 26 0.0907140070230199 0.0 0.0 4.877097151775263E-4 0.0 27 0.09266484588373 0.0 0.0 4.877097151775263E-4 0.0 28 0.11217323449083105 0.0 0.0 4.877097151775263E-4 0.0 29 0.1146117830667187 0.0 0.0 4.877097151775263E-4 0.0 30 0.12095200936402653 0.0 0.0 4.877097151775263E-4 0.0 31 0.12631681623097932 0.0 0.0 4.877097151775263E-4 0.0 32 0.12875536480686695 0.0 0.0 4.877097151775263E-4 0.0 33 0.13216933281310964 0.0 0.0 4.877097151775263E-4 0.0 34 0.13753413968006242 0.0 0.0 4.877097151775263E-4 0.0 35 0.15704252828716347 0.0 0.0 4.877097151775263E-4 0.0 36 0.16240733515411626 0.0 0.0 4.877097151775263E-4 0.0 37 0.16630901287553648 0.0 0.0 4.877097151775263E-4 0.0 38 0.17167381974248927 0.0 0.0 4.877097151775263E-4 0.0 39 0.176063207179087 0.0 0.0 4.877097151775263E-4 0.0 40 0.17947717518532968 0.0 0.0 4.877097151775263E-4 0.0 41 0.18484198205228247 0.0 0.0 4.877097151775263E-4 0.0 42 0.18923136948888022 0.0 0.0 4.877097151775263E-4 0.0 43 0.19264533749512291 0.0 0.0 4.877097151775263E-4 0.0 44 0.19752243464689817 0.0 0.0 4.877097151775263E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCGGT 15 0.007981998 50.68183 49 CATATTA 20 3.9056747E-4 50.681828 50 CGCGAAC 20 3.9056747E-4 50.681828 49 AGTCCGC 15 0.008227981 50.294277 45 ACGTTAA 15 0.008227981 50.294277 47 TATATAA 15 0.008227981 50.294277 46 TTTGTCG 20 4.056516E-4 50.294273 44 ATTATAC 15 0.008244042 50.269478 42 CACGAGT 15 0.008276234 50.219948 39 ATGCTCG 15 0.008300439 50.182873 36 CGGAATA 20 4.1061494E-4 50.17052 35 GTAAATT 15 0.00830852 50.17052 35 AGTAAAT 15 0.008349008 50.10887 34 TATGCGT 20 4.1411788E-4 50.084255 32 ATGCGTT 20 4.1411788E-4 50.084255 33 CCTATGC 15 0.008365245 50.084255 33 CACAAAT 15 0.008365245 50.084255 32 GTATGCG 20 4.1462024E-4 50.071953 31 GAGCATA 20 4.1512307E-4 50.059658 30 TACACAA 20 4.1512307E-4 50.059658 30 >>END_MODULE