FastQCFastQC Report
Fri 17 Jun 2016
SRR1526994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119128
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT20221.6973339601101336No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2410.20230340474111877No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1870.1569740111476731No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.15193741185951246No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.12255724934524209No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1350.11332348398361426No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1280.1074474514807602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTCG150.004527380758.47772656
GTAGGTG303.6744314E-758.1324354
AGTAGGT351.5954356E-858.1324353
AGGGATA600.058.1324356
GGGATAC201.9805385E-458.132437
AAACTAC150.004681485757.985748
TCGCATA202.005456E-457.985744
TCACCTA150.004681485757.985740
ATTACGC150.004681485757.985751
GTCTATA202.005456E-457.985741
CCTAAAC202.005456E-457.985751
AGACGGT150.004681485757.985747
TACGCCA202.005456E-457.985753
CTAAACA202.005456E-457.985752
GTGTCTA150.004681485757.985739
CTCGCAT202.005456E-457.985743
TTACGCC150.004681485757.985752
GATTACG202.005456E-457.985750
TGCGTCT150.004681485757.985738
GACTACT150.004681485757.985744
Produced by FastQC (version 0.11.5)