FastQCFastQC Report
Fri 17 Jun 2016
SRR1526993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171318
Sequences flagged as poor quality0
Sequence length64
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT46992.7428524731785338No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6140.3583978332691252No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5310.3099499176969145No Hit
CGAGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5180.3023616899566887No Hit
CGAGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4750.27726216743132653No Hit
CGAGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4670.27259248882195686No Hit
CGAGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4340.2533300645583068No Hit
CGAGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA4330.2527463547321356No Hit
CGAGTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3700.2159726356833491No Hit
CGAGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3610.21071924724780816No Hit
CGAGTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3440.20079618020289752No Hit
CGAGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA3280.19145682298415811No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA3080.17978262646073384No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3070.1791989166345626No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT2970.1733618183728505No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2920.1704432692419944No Hit
CGAGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG2800.16343875132793986No Hit
CGAGTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2750.1605202021970838No Hit
CGAGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2740.15993649237091256No Hit
CGAGTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC2720.15876907271857016No Hit
CGAGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2670.15585052358771406No Hit
CGAGTAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC2660.15526681376154286No Hit
CGAGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2480.144760036890461No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.14125777793343372No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.13658809932406402No Hit
CGAGTAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT2330.1360043894978928No Hit
CGAGTAGGGGTGCTCGAGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC2310.13483696984555038No Hit
CGAGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG2230.13016729123618068No Hit
CGAGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGC2110.12316277332212609No Hit
CGAGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2100.12257906349595489No Hit
CGAGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA2090.12199535366978367No Hit
CGAGTAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC2050.11966051436509881No Hit
CGAGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2030.11849309471275639No Hit
CGAGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG2000.11674196523424275No Hit
CGAGTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT1980.11557454558190033No Hit
CGAGTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC1880.10973744732018818No Hit
CGAGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1850.10798631784167455No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1790.10448405888464726No Hit
CGAGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC1760.10273292940613363No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC1750.10214921957996241No Hit
CGAGTAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1740.10156550975379118No Hit
CGAGTAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTACCGAACATCTAGA1740.10156550975379118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAAG150.00460517958.237858
AGGTCTA150.00460517958.237858
TATCAAT201.9681902E-458.22068852
AACGGTC201.9681902E-458.22068855
AGGTCGA201.9681902E-458.22068855
AGATCAT150.00461054358.22068456
GCGACTG150.00461054358.22068452
CATGATA150.00461054358.22068451
TAGGTCT150.00461054358.22068457
TCAATGC150.00461054358.22068454
ACAACGG201.9710523E-458.20358353
CTCATAT201.9710523E-458.20358353
CCACTCG150.004621284558.1864950
CTATGTT201.9767864E-458.16940346
CTATTCG201.9767864E-458.16940346
ATCGGCA150.00462666258.16940346
AGTAACA150.00462666258.16940348
TATCACC201.9796586E-458.15233644
GCAACTA150.004632044658.15233244
ATGTGCC150.004632044658.15233245
Produced by FastQC (version 0.11.5)