FastQCFastQC Report
Fri 17 Jun 2016
SRR1526991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155107
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG52083.3576821162165476No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10330.6659918636812008No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8160.526088442172178No Hit
CGAGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4690.30237191100337185No Hit
CGAGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG4180.26949138336761075No Hit
CGAGTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4050.2611100724016324No Hit
CGAGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA3580.23080840967848No Hit
CGAGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA3470.22371653116880605No Hit
CGAGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3430.22113766625619732No Hit
CGAGTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT3270.21082220660576245No Hit
CGAGTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT3270.21082220660576245No Hit
CGAGTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT3250.20953277414945812No Hit
CGAGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC3120.2011514631834798No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC3080.19857259827087106No Hit
CGAGTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2820.18180997633891444No Hit
CGAGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2620.16891565177587087No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.1669815030914143No Hit
CGAGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2440.15731075966913163No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2420.15602132721282727No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT2420.15602132721282727No Hit
CGAGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2390.15408717852837073No Hit
CGAGTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC2310.1489294487031533No Hit
CGAGTAGGGGTGCTCGAGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGG2250.14506115133424022No Hit
CGAGTAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC2130.1373245565964141No Hit
CGAGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG2040.1315221105430445No Hit
CGAGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2040.1315221105430445No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT1980.1276538131741314No Hit
CGAGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1960.12636438071782705No Hit
CGAGTAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1950.12571966448967486No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC1880.12120665089260962No Hit
CGAGTAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT1860.11991721843630526No Hit
CGAGTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA1850.11927250220815308No Hit
CGAGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT1730.11153590747032693No Hit
CGAGTAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC1720.11089119124217475No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1710.11024647501402259No Hit
CGAGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1700.10960175878587039No Hit
CGAGTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC1680.10831232632956606No Hit
CGAGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCG1590.10250988027619642No Hit
CGAGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG1580.10186516404804426No Hit
CGAGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1570.10122044781989209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAT150.0079578850.7146650
CCAATAA203.890193E-450.7146650
TGACGAT150.0079578850.7146650
CACTATA150.0079578850.7146649
TTGACGA150.0079578850.7146649
CCTAAAC150.00796823150.69799448
TTAGGCC150.00821966550.30127346
TAAAACC150.0082408850.26849444
CTTGACC204.0636773E-450.26849444
GGTTATC204.0636773E-450.26849444
TATAAGA150.0082408850.26849444
CAATTAT252.0141271E-550.2684944
CTATAAG150.00825150450.25211743
TTAAAAC150.00825150450.25211743
TGACTAC150.00825150450.25211743
ACAATTA252.0180154E-550.25211343
CTGACTA150.00826213750.23575642
AGGTATT150.00828343450.2030641
TCTAACA150.00828343450.2030641
CCATTAG150.00828343450.2030641
Produced by FastQC (version 0.11.5)